O24676 · TECA1_CUPXP
- ProteinChlorobenzene dioxygenase subunit alpha
- GenetecA1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids449 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Part of the oxygenase component of the chlorobenzene dioxygenase system that catalyzes the dihydroxylation of a range of aromatic compounds, including chlorinated benzenes and toluenes, and dinuclear aromatics such as biphenyl and dibenzo-p-dioxin (PubMed:11526005, PubMed:9249026, PubMed:9791099).
The alpha subunit is responsible for substrate specificity (PubMed:9791099).
The alpha subunit is responsible for substrate specificity (PubMed:9791099).
Catalytic activity
- chlorobenzene + NADH + O2 + H+ = (1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol + NAD+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 [2Fe-2S] cluster per subunit.
Note: Binds 1 Fe cation per subunit.
Pathway
Aromatic compound metabolism.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 96 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 98 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 116 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 119 | [2Fe-2S] cluster (UniProtKB | ChEBI) | |||
Binding site | 222 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 228 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 376 | Fe cation (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2 iron, 2 sulfur cluster binding | |
Molecular Function | dioxygenase activity | |
Molecular Function | iron ion binding | |
Biological Process | catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameChlorobenzene dioxygenase subunit alpha
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Cupriavidus
Accessions
- Primary accessionO24676
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000453515 | 1-449 | Chlorobenzene dioxygenase subunit alpha | ||
Interaction
Subunit
This dioxygenase system consists of four proteins: the two subunits of the oxygenase component (TecA1 and TecA2), a ferredoxin (TecA3) and a ferredoxin reductase (TecA4).
Structure
Sequence
- Sequence statusComplete
- Length449
- Mass (Da)50,541
- Last updated1998-01-01 v1
- MD5 ChecksumFF77164396FE6B55650412255CE83E3D
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U78099 EMBL· GenBank· DDBJ | AAC46390.1 EMBL· GenBank· DDBJ | Genomic DNA |