O24606 · EIN3_ARATH
- ProteinProtein ETHYLENE INSENSITIVE 3
- GeneEIN3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids628 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription factor acting as a positive regulator in the ethylene response pathway, by promoting histone acetylation in an ENAP1-dependent manner, thus accelerating the expression of ethylene-responsive genes (PubMed:28874528).
Binds DNA (PubMed:26352699).
Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene
Binds DNA (PubMed:26352699).
Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene
Miscellaneous
Loss-of-function mutations (ein3-1 and ein3-2) in the gene lead to the suppression of ethylene-mediated effects including gene expression, the triple response, cell growth inhibition, and accelerated senescence.
Activity regulation
Activated by phosphorylation by MPK3 and MPK6 (PubMed:18273012).
Down-regulated by KIN10 that controls its protein stability under a phosphorylation-dependent manner (PubMed:28600557).
Satnilitzed during hypoxia (e.g. submergences) via a ceramides-triggered and CTR1-dependent manner (PubMed:25822663).
Down-regulated by KIN10 that controls its protein stability under a phosphorylation-dependent manner (PubMed:28600557).
Satnilitzed during hypoxia (e.g. submergences) via a ceramides-triggered and CTR1-dependent manner (PubMed:25822663).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | chromatin binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | histone binding | |
Molecular Function | kinase binding | |
Molecular Function | transcription cis-regulatory region binding | |
Biological Process | defense response to bacterium | |
Biological Process | epigenetic regulation of gene expression | |
Biological Process | ethylene-activated signaling pathway | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | regulation of L-ascorbic acid biosynthetic process | |
Biological Process | response to ethylene | |
Biological Process | response to hypoxia | |
Biological Process | sugar mediated signaling pathway |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameProtein ETHYLENE INSENSITIVE 3
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionO24606
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 245 | In ein3-3; suppression of DNA-binding. | ||||
Sequence: K → N |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 30 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000113498 | 1-628 | Protein ETHYLENE INSENSITIVE 3 | |||
Sequence: MMFNEMGMCGNMDFFSSGSLGEVDFCPVPQAEPDSIVEDDYTDDEIDVDELERRMWRDKMRLKRLKEQDKGKEGVDAAKQRQSQEQARRKKMSRAQDGILKYMLKMMEVCKAQGFVYGIIPENGKPVTGASDNLREWWKDKVRFDRNGPAAITKYQAENNIPGIHEGNNPIGPTPHTLQELQDTTLGSLLSALMQHCDPPQRRFPLEKGVPPPWWPNGKEDWWPQLGLPKDQGPAPYKKPHDLKKAWKVGVLTAVIKHMFPDIAKIRKLVRQSKCLQDKMTAKESATWLAIINQEESLARELYPESCPPLSLSGGSCSLLMNDCSQYDVEGFEKESHYEVEELKPEKVMNSSNFGMVAKMHDFPVKEEVPAGNSEFMRKRKPNRDLNTIMDRTVFTCENLGCAHSEISRGFLDRNSRDNHQLACPHRDSRLPYGAAPSRFHVNEVKPVVGFPQPRPVNSVAQPIDLTGIVPEDGQKMISELMSMYDRNVQSNQTSMVMENQSVSLLQPTVHNHQEHLQFPGNMVEGSFFEDLNIPNRANNNNSSNNQTFFQGNNNNNNVFKFDTADHNNFEAAHNNNNNSSGNRFQLVFDSTPFDMASFDYRDDMSMPGVVGTMDGMQQKQQDVSIWF |
Post-translational modification
Phosphorylated by KIN10.
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Acts as a homodimer to bind the primary ethylene response element. Interacts with TAF12B. Interacts with KIN10 (PubMed:28600557).
Binds to ENAP1 in the presence of ethylene; this reaction facilitates its association with histone (PubMed:27694846, PubMed:28874528).
Binds to ENAP1 in the presence of ethylene; this reaction facilitates its association with histone (PubMed:27694846, PubMed:28874528).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | O24606 | EBF1 Q9SKK0 | 4 | EBI-593576, EBI-401198 | |
BINARY | O24606 | EBF2 Q708Y0 | 3 | EBI-593576, EBI-593623 | |
BINARY | O24606 | HDA6 Q9FML2 | 2 | EBI-593576, EBI-639608 | |
BINARY | O24606 | TIFY10A Q9LMA8 | 4 | EBI-593576, EBI-1388539 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for coiled coil, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 38-68 | |||||
Sequence: EDDYTDDEIDVDELERRMWRDKMRLKRLKEQ | ||||||
Region | 66-92 | Disordered | ||||
Sequence: KEQDKGKEGVDAAKQRQSQEQARRKKM | ||||||
Region | 174-306 | DNA-binding domain | ||||
Sequence: TPHTLQELQDTTLGSLLSALMQHCDPPQRRFPLEKGVPPPWWPNGKEDWWPQLGLPKDQGPAPYKKPHDLKKAWKVGVLTAVIKHMFPDIAKIRKLVRQSKCLQDKMTAKESATWLAIINQEESLARELYPES |
Sequence similarities
Belongs to the EIN3 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length628
- Mass (Da)71,421
- Last updated1998-01-01 v1
- Checksum8DA269C9F2A3BFD3
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF004216 EMBL· GenBank· DDBJ | AAC49749.1 EMBL· GenBank· DDBJ | mRNA | ||
AF004217 EMBL· GenBank· DDBJ | AAC49750.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB025629 EMBL· GenBank· DDBJ | BAB02485.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE76421.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY069875 EMBL· GenBank· DDBJ | AAL47431.2 EMBL· GenBank· DDBJ | mRNA |