O24474 · TPSD2_ABIGR
- ProteinMyrcene synthase, chloroplastic
- Geneag2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids627 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis.
Miscellaneous
The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence.
Catalytic activity
- (2E)-geranyl diphosphate = beta-myrcene + diphosphate
Cofactor
Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 3 Mg2+ or Mn2+ ions per subunit.
Pathway
Terpene metabolism; oleoresin biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | magnesium ion binding | |
Molecular Function | myrcene synthase activity | |
Biological Process | diterpenoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMyrcene synthase, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Pinopsida > Pinidae > Conifers I > Pinales > Pinaceae > Abies
Accessions
- Primary accessionO24474
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-52 | Chloroplast | ||||
Sequence: MALVSISPLASKSCLRKSLISSIHEHKPPYRTIPNLGMRRRGKSVTPSMSIS | ||||||
Chain | PRO_0000033627 | 53-627 | Myrcene synthase, chloroplastic | |||
Sequence: LATAAPDDGVQRRIGDYHSNIWDDDFIQSLSTPYGEPSYQERAERLIVEVKKIFNSMYLDDGRLMSSFNDLMQRLWIVDSVERLGIARHFKNEITSALDYVFRYWEENGIGCGRDSIVTDLNSTALGFRTLRLHGYTVSPEVLKAFQDQNGQFVCSPGQTEGEIRSVLNLYRASLIAFPGEKVMEEAEIFSTRYLKEALQKIPVSALSQEIKFVMEYGWHTNLPRLEARNYIDTLEKDTSAWLNKNAGKKLLELAKLEFNIFNSLQQKELQYLLRWWKESDLPKLTFARHRHVEFYTLASCIAIDPKHSAFRLGFAKMCHLVTVLDDIYDTFGTIDELELFTSAIKRWNSSEIEHLPEYMKCVYMVVFETVNELTREAEKTQGRNTLNYVRKAWEAYFDSYMEEAKWISNGYLPMFEEYHENGKVSSAYRVATLQPILTLNAWLPDYILKGIDFPSRFNDLASSFLRLRGDTRCYKADRDRGEEASCISCYMKDNPGSTEEDALNHINAMVNDIIKELNWELLRSNDNIPMLAKKHAFDITRALHHLYIYRDGFSVANKETKKLVMETLLESMLF |
Expression
Induction
By wounding.
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 378-382 | DDXXD motif | ||||
Sequence: DDIYD |
Domain
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities
Belongs to the terpene synthase family. Tpsd subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length627
- Mass (Da)72,479
- Last updated1998-01-01 v1
- Checksum2E0DA492E0C971FD
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U87908 EMBL· GenBank· DDBJ | AAB71084.1 EMBL· GenBank· DDBJ | mRNA |