O23087 · ECA2_ARATH
- ProteinCalcium-transporting ATPase 2, endoplasmic reticulum-type
- GeneECA2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1054 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment.
Catalytic activity
- ATP + Ca2+(in) + H2O = ADP + Ca2+(out) + H+ + phosphate
CHEBI:30616 + Ca2+ (in)CHEBI:29108+ CHEBI:15377 = CHEBI:456216 + Ca2+ (out)CHEBI:29108+ CHEBI:15378 + CHEBI:43474
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 321 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 322 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 324 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 326 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 368 | 4-aspartylphosphate intermediate | ||||
Sequence: D | ||||||
Binding site | 727 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 731 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 793 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 796 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 821 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 824 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 825 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 825 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 960 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | membrane | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | metal ion binding | |
Molecular Function | P-type calcium transporter activity | |
Biological Process | calcium ion transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCalcium-transporting ATPase 2, endoplasmic reticulum-type
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionO23087
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-53 | Cytoplasmic | ||||
Sequence: MEEEKSFSAWSWSVEQCLKEYKTRLDKGLTSEDVQIRRQKYGFNELAKEKGKP | ||||||
Transmembrane | 54-74 | Helical | ||||
Sequence: LWHLVLEQFDDTLVKILLGAA | ||||||
Topological domain | 75-98 | Lumenal | ||||
Sequence: FISFVLAFLGEEHGSGSGFEAFVE | ||||||
Transmembrane | 99-118 | Helical | ||||
Sequence: PFVIVLILILNAVVGVWQES | ||||||
Topological domain | 119-262 | Cytoplasmic | ||||
Sequence: NAEKALEALKEMQCESAKVLRDGNVLPNLPARELVPGDIVELNVGDKVPADMRVSGLKTSTLRVEQSSLTGEAMPVLKGANLVVMDDCELQGKENMVFAGTTVVNGSCVCIVTSIGMDTEIGKIQRQIHEASLEESETPLKKKL | ||||||
Transmembrane | 263-282 | Helical | ||||
Sequence: DEFGSRLTTAICIVCVLVWM | ||||||
Topological domain | 283-312 | Lumenal | ||||
Sequence: INYKNFVSWDVVDGYKPVNIKFSFEKCTYY | ||||||
Transmembrane | 313-330 | Helical | ||||
Sequence: FKIAVALAVAAIPEGLPA | ||||||
Topological domain | 331-782 | Cytoplasmic | ||||
Sequence: VITTCLALGTRKMAQKNAIVRKLPSVETLGCTTVICSDKTGTLTTNQMSATEFFTLGGKTTTTRVFSVSGTTYDPKDGGIVDWGCNNMDANLQAVAEICSICNDAGVFYEGKLFRATGLPTEAALKVLVEKMGIPEKKNSENIEEVTNFSDNGSSVKLACCDWWNKRSKKVATLEFDRVRKSMSVIVSEPNGQNRLLVKGAAESILERSSFAQLADGSLVALDESSREVILKKHSEMTSKGLRCLGLAYKDELGEFSDYSSEEHPSHKKLLDPSSYSNIETNLIFVGVVGLRDPPREEVGRAIEDCRDAGIRVMVITGDNKSTAEAICCEIRLFSENEDLSQSSFTGKEFMSLPASRRSEILSKSGGKVFSRAEPRHKQEIVRMLKEMGEIVAMTGDGVNDAPALKLADIGIAMGITGTEVAKEASDMVLADDNFSTIVSAVAEGRSIYNNM | ||||||
Transmembrane | 783-802 | Helical | ||||
Sequence: KAFIRYMISSNVGEVISIFL | ||||||
Topological domain | 803-812 | Lumenal | ||||
Sequence: TAALGIPECM | ||||||
Transmembrane | 813-833 | Helical | ||||
Sequence: IPVQLLWVNLVTDGPPATALG | ||||||
Topological domain | 834-853 | Cytoplasmic | ||||
Sequence: FNPADIDIMKKPPRKSDDCL | ||||||
Transmembrane | 854-876 | Helical | ||||
Sequence: IDSWVLIRYLVIGSYVGVATVGI | ||||||
Topological domain | 877-949 | Lumenal | ||||
Sequence: FVLWYTQASFLGISLISDGHTLVSFTQLQNWSECSSWGTNFTATPYTVAGGLRTIAFENNPCDYFTLGKVKPM | ||||||
Transmembrane | 950-969 | Helical | ||||
Sequence: TLSLTVLVAIEMFNSLNALS | ||||||
Topological domain | 970-982 | Cytoplasmic | ||||
Sequence: EDNSLLTMPPWRN | ||||||
Transmembrane | 983-1001 | Helical | ||||
Sequence: PWLLVAMTVSFALHCVILY | ||||||
Topological domain | 1002-1016 | Lumenal | ||||
Sequence: VPFLANVFGIVPLSF | ||||||
Transmembrane | 1017-1037 | Helical | ||||
Sequence: REWFVVILVSFPVILIDEALK | ||||||
Topological domain | 1038-1054 | Cytoplasmic | ||||
Sequence: FIGRCRRTRIKKKIKTM |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000046406 | 1-1054 | Calcium-transporting ATPase 2, endoplasmic reticulum-type | |||
Sequence: MEEEKSFSAWSWSVEQCLKEYKTRLDKGLTSEDVQIRRQKYGFNELAKEKGKPLWHLVLEQFDDTLVKILLGAAFISFVLAFLGEEHGSGSGFEAFVEPFVIVLILILNAVVGVWQESNAEKALEALKEMQCESAKVLRDGNVLPNLPARELVPGDIVELNVGDKVPADMRVSGLKTSTLRVEQSSLTGEAMPVLKGANLVVMDDCELQGKENMVFAGTTVVNGSCVCIVTSIGMDTEIGKIQRQIHEASLEESETPLKKKLDEFGSRLTTAICIVCVLVWMINYKNFVSWDVVDGYKPVNIKFSFEKCTYYFKIAVALAVAAIPEGLPAVITTCLALGTRKMAQKNAIVRKLPSVETLGCTTVICSDKTGTLTTNQMSATEFFTLGGKTTTTRVFSVSGTTYDPKDGGIVDWGCNNMDANLQAVAEICSICNDAGVFYEGKLFRATGLPTEAALKVLVEKMGIPEKKNSENIEEVTNFSDNGSSVKLACCDWWNKRSKKVATLEFDRVRKSMSVIVSEPNGQNRLLVKGAAESILERSSFAQLADGSLVALDESSREVILKKHSEMTSKGLRCLGLAYKDELGEFSDYSSEEHPSHKKLLDPSSYSNIETNLIFVGVVGLRDPPREEVGRAIEDCRDAGIRVMVITGDNKSTAEAICCEIRLFSENEDLSQSSFTGKEFMSLPASRRSEILSKSGGKVFSRAEPRHKQEIVRMLKEMGEIVAMTGDGVNDAPALKLADIGIAMGITGTEVAKEASDMVLADDNFSTIVSAVAEGRSIYNNMKAFIRYMISSNVGEVISIFLTAALGIPECMIPVQLLWVNLVTDGPPATALGFNPADIDIMKKPPRKSDDCLIDSWVLIRYLVIGSYVGVATVGIFVLWYTQASFLGISLISDGHTLVSFTQLQNWSECSSWGTNFTATPYTVAGGLRTIAFENNPCDYFTLGKVKPMTLSLTVLVAIEMFNSLNALSEDNSLLTMPPWRNPWLLVAMTVSFALHCVILYVPFLANVFGIVPLSFREWFVVILVSFPVILIDEALKFIGRCRRTRIKKKIKTM |
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length1,054
- Mass (Da)115,830
- Last updated1998-01-01 v1
- ChecksumB0D7F7237A3496AF
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ132387 EMBL· GenBank· DDBJ | CAA10659.1 EMBL· GenBank· DDBJ | mRNA | ||
AF013294 EMBL· GenBank· DDBJ | AAB62850.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL161472 EMBL· GenBank· DDBJ | CAB80899.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE81953.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | ANM67918.1 EMBL· GenBank· DDBJ | Genomic DNA |