O22527 · CLH1_ARATH

Function

function

Catalyzes the hydrolysis of ester bond in chlorophyll to yield chlorophyllide and phytol (PubMed:10611389, PubMed:11950974).
Shows a preferential activity toward chlorophyll a (PubMed:11950974).
Does not seem to be required for chlorophyll degradation during senescence (PubMed:17996203, PubMed:18349515, PubMed:31779896).
May modulate the balance between different plant defense pathways (PubMed:15598807).

Miscellaneous

It has been proposed that some chlorophyllase might be transported to vacuole via the endoplasmic reticulum where they might be glycosylated (Probable). Unlike CLH2, the expression of this protein is dependent on the presence of a functional COI1 protein (PubMed:11950974).
Plants silencing CLH1 exhibit reduced size, and decreased levels of chlorophyll and chlorophyllide (PubMed:15598807).
Plants silencing CLH1 exhibit enhanced resistance and salicylate (SA) levels, and decreased jasmonate levels (JA) upon infection with the bacterial pathogen Pectobacterium carotovorum (PubMed:15598807).
Plants silencing CLH1 exhibit enhanced susceptibility to infection by the fungal pathogen Alternaria brassicicola (PubMed:15598807).

Catalytic activity

Pathway

Porphyrin-containing compound metabolism; chlorophyll degradation.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site138Nucleophile
Active site168Charge relay system
Active site243Charge relay system

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentnucleus
Cellular Componentplant-type vacuole
Molecular Functionchlorophyllase activity
Biological Processchlorophyll catabolic process
Biological Processdefense response to bacterium
Biological Processdefense response to fungus

Keywords

Enzyme and pathway databases

Protein family/group databases

Chemistry

Names & Taxonomy

Protein names

  • Recommended name
    Chlorophyllase-1
  • EC number
  • Short names
    AtCLH1
  • Alternative names
    • Chlorophyll-chlorophyllido hydrolase 1
      (Chlase 1
      )
    • Coronatine-induced protein 1
      (CORI1
      )

Gene names

    • Name
      CLH1
    • Synonyms
      ATHCOR1
      , COR1
    • ORF names
      F6F9.28
    • Ordered locus names
      At1g19670

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia
    • cv. Landsberg erecta
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    O22527

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000903861-324Chlorophyllase-1

Proteomic databases

Expression

Tissue specificity

Expressed in seedlings, leaves, flowers and siliques, but not in roots.

Induction

Induced by methyl jasmonate, coronatine, a phytotoxin produced by some plant-pathogenic bacteria or rapidly after wounding, with a peak after 30 minutes and a return to the basal level in the following 4 hours (PubMed:9501136).
Induced by methyl jasmonate (JA), wounding, infection with the bacterial pathogen Pectobacterium carotovorum, and with the fungal pathogen Alternaria brassicicola (PubMed:15598807).
Induced by transition from dark to white light (PubMed:21896889).
Down-regulated by dark (PubMed:21896889).

Developmental stage

Constitutively expressed in flowers with a higher level at the stage of buds.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif136-140GXSXG

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    324
  • Mass (Da)
    34,855
  • Last updated
    1998-01-01 v1
  • MD5 Checksum
    C7069514A956E15D9A6D0490BAD45E70
MAAIEDSPTFSSVVTPAAFEIGSLPTTEIPVDPVENDSTAPPKPVRITCPTVAGTYPVVLFFHGFYLRNYFYSDVLNHIASHGYILVAPQLCKLLPPGGQVEVDDAGSVINWASENLKAHLPTSVNANGKYTSLVGHSRGGKTAFAVALGHAATLDPSITFSALIGIDPVAGTNKYIRTDPHILTYKPESFELDIPVAVVGTGLGPKWNNVMPPCAPTDLNHEEFYKECKATKAHFVAADYGHMDMLDDDLPGFVGFMAGCMCKNGQRKKSEMRSFVGGIVVAFLKYSLWGEKAEIRLIVKDPSVSPAKLDPSPELEEASGIFV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF021244
EMBL· GenBank· DDBJ
AAC13947.1
EMBL· GenBank· DDBJ
mRNA
AC007797
EMBL· GenBank· DDBJ
AAG12547.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE29881.1
EMBL· GenBank· DDBJ
Genomic DNA
AY099815
EMBL· GenBank· DDBJ
AAM20666.1
EMBL· GenBank· DDBJ
mRNA
BT000309
EMBL· GenBank· DDBJ
AAN15628.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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