O22527 · CLH1_ARATH
- ProteinChlorophyllase-1
- GeneCLH1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids324 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the hydrolysis of ester bond in chlorophyll to yield chlorophyllide and phytol (PubMed:10611389, PubMed:11950974).
Shows a preferential activity toward chlorophyll a (PubMed:11950974).
Does not seem to be required for chlorophyll degradation during senescence (PubMed:17996203, PubMed:18349515, PubMed:31779896).
May modulate the balance between different plant defense pathways (PubMed:15598807).
Shows a preferential activity toward chlorophyll a (PubMed:11950974).
Does not seem to be required for chlorophyll degradation during senescence (PubMed:17996203, PubMed:18349515, PubMed:31779896).
May modulate the balance between different plant defense pathways (PubMed:15598807).
Miscellaneous
It has been proposed that some chlorophyllase might be transported to vacuole via the endoplasmic reticulum where they might be glycosylated (Probable). Unlike CLH2, the expression of this protein is dependent on the presence of a functional COI1 protein (PubMed:11950974).
Plants silencing CLH1 exhibit reduced size, and decreased levels of chlorophyll and chlorophyllide (PubMed:15598807).
Plants silencing CLH1 exhibit enhanced resistance and salicylate (SA) levels, and decreased jasmonate levels (JA) upon infection with the bacterial pathogen Pectobacterium carotovorum (PubMed:15598807).
Plants silencing CLH1 exhibit enhanced susceptibility to infection by the fungal pathogen Alternaria brassicicola (PubMed:15598807).
Plants silencing CLH1 exhibit reduced size, and decreased levels of chlorophyll and chlorophyllide (PubMed:15598807).
Plants silencing CLH1 exhibit enhanced resistance and salicylate (SA) levels, and decreased jasmonate levels (JA) upon infection with the bacterial pathogen Pectobacterium carotovorum (PubMed:15598807).
Plants silencing CLH1 exhibit enhanced susceptibility to infection by the fungal pathogen Alternaria brassicicola (PubMed:15598807).
Catalytic activity
- a chlorophyll + H2O = a chlorophyllide + phytol + H+This reaction proceeds in the forward direction.
Pathway
Porphyrin-containing compound metabolism; chlorophyll degradation.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 138 | Nucleophile | |||
Active site | 168 | Charge relay system | |||
Active site | 243 | Charge relay system | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Cellular Component | plant-type vacuole | |
Molecular Function | chlorophyllase activity | |
Biological Process | chlorophyll catabolic process | |
Biological Process | defense response to bacterium | |
Biological Process | defense response to fungus |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameChlorophyllase-1
- EC number
- Short namesAtCLH1
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionO22527
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000090386 | 1-324 | Chlorophyllase-1 | ||
Proteomic databases
Expression
Tissue specificity
Expressed in seedlings, leaves, flowers and siliques, but not in roots.
Induction
Induced by methyl jasmonate, coronatine, a phytotoxin produced by some plant-pathogenic bacteria or rapidly after wounding, with a peak after 30 minutes and a return to the basal level in the following 4 hours (PubMed:9501136).
Induced by methyl jasmonate (JA), wounding, infection with the bacterial pathogen Pectobacterium carotovorum, and with the fungal pathogen Alternaria brassicicola (PubMed:15598807).
Induced by transition from dark to white light (PubMed:21896889).
Down-regulated by dark (PubMed:21896889).
Induced by methyl jasmonate (JA), wounding, infection with the bacterial pathogen Pectobacterium carotovorum, and with the fungal pathogen Alternaria brassicicola (PubMed:15598807).
Induced by transition from dark to white light (PubMed:21896889).
Down-regulated by dark (PubMed:21896889).
Developmental stage
Constitutively expressed in flowers with a higher level at the stage of buds.
Gene expression databases
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length324
- Mass (Da)34,855
- Last updated1998-01-01 v1
- MD5 ChecksumC7069514A956E15D9A6D0490BAD45E70
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF021244 EMBL· GenBank· DDBJ | AAC13947.1 EMBL· GenBank· DDBJ | mRNA | ||
AC007797 EMBL· GenBank· DDBJ | AAG12547.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE29881.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY099815 EMBL· GenBank· DDBJ | AAM20666.1 EMBL· GenBank· DDBJ | mRNA | ||
BT000309 EMBL· GenBank· DDBJ | AAN15628.1 EMBL· GenBank· DDBJ | mRNA |