O22436 · CHLI_TOBAC
- ProteinMagnesium-chelatase subunit ChlI, chloroplastic
- GeneCHLI
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids426 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. The magnesium-chelatase is a complex of three subunits, CHLI, CHLD and CHLH. The reaction takes place in two steps, with an ATP-dependent activation followed by an ATP-dependent chelation step (By similarity).
Catalytic activity
- ATP + H2O + Mg2+ + protoporphyrin IX = ADP + 3 H+ + Mg-protoporphyrin IX + phosphate
Activity regulation
Redox regulation; active in reducing conditions, inactive in oxidizing conditions. Thioredoxins f and m mediate the reversible reductive activation of oxidized CHLI (By similarity).
Pathway
Porphyrin-containing compound metabolism; chlorophyll biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast stroma | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | magnesium chelatase activity | |
Biological Process | chlorophyll biosynthetic process | |
Biological Process | photosynthesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMagnesium-chelatase subunit ChlI, chloroplastic
- EC number
- Short namesMg-chelatase subunit I-1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Nicotianoideae > Nicotianeae > Nicotiana
Accessions
- Primary accessionO22436
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-50 | Chloroplast | ||||
Sequence: MASLLGTSSSAAAAILASTPLSSRSCKPAVFSLFPSSGQSQGRKFYGGIR | ||||||
Chain | PRO_0000002804 | 51-426 | Magnesium-chelatase subunit ChlI, chloroplastic | |||
Sequence: VPVKKGRSQFHVAISNVATEINLLKNRVRNLLEESQRPVYPFAAIVGQDEMKLCLLLNVIDPKIGGVMIMGDRGTGKSTTVRSLVDLLPEIKVISGDPFNSDPDDQEVMSAEVRDKLRSGQQLPISRTKINMVDLPLGATEDRVCGTIDIEKALTEGVKAFEPGLLAKANRGILYVDEVNLLDDHLVDVLLDSAASGWNTVEREGISISHPARFILIGSGNPEEGELRPQLLDRFGMHAQVGTVRDAELRVKIVEERARFDKNPKEFRESYKAEQEKLQNQIDSARNALSAVTIDHDLRVKISKVCAELNVDGLRGDIVTNRAARALAALKGRDKVTPEDIATVIPNCLRHRLRKDPLESIDSGVLVVEKFYEVFA | ||||||
Disulfide bond | 104↔195 | |||||
Sequence: CLLLNVIDPKIGGVMIMGDRGTGKSTTVRSLVDLLPEIKVISGDPFNSDPDDQEVMSAEVRDKLRSGQQLPISRTKINMVDLPLGATEDRVC | ||||||
Disulfide bond | 356↔398 | Inhibitory under oxidizing conditions | ||||
Sequence: CAELNVDGLRGDIVTNRAARALAALKGRDKVTPEDIATVIPNC |
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Strongly expressed in young leaves and to a lesser extent in mature leaves.
Interaction
Subunit
The magnesium chelatase complex is a heterotrimer consisting of subunits CHLI, CHLD and CHLH.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length426
- Mass (Da)46,627
- Last updated1998-01-01 v1
- Checksum42B7A6F04E1C3274
Keywords
- Technical term