O22040 · ANP1_ARATH
- ProteinMitogen-activated protein kinase kinase kinase ANP1
- GeneANP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids666 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
May be involved in an oxidative stress-mediated signaling cascade that phosphorylates downstream MAP kinases MPK3 and MPK6. May suppress auxin signaling that promotes cell cycle. Functionally redundant to ANP2 and ANP3 in the positive regulation of cytokinesis.
Miscellaneous
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | MAP kinase kinase kinase activity | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | protein autophosphorylation | |
Biological Process | response to oxidative stress |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMitogen-activated protein kinase kinase kinase ANP1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionO22040
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 98 | Loss of kinase activity. | ||||
Sequence: K → M |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 44 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000086270 | 1-666 | Mitogen-activated protein kinase kinase kinase ANP1 | |||
Sequence: MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGVLADKITSCIRKSKIFIKPSFSPPPPANTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQINNTKSTPDSTCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGDEDDMCLIDDENFLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDESPGNGEKESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLITFSPSCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSRELNINNGGDEASQDGVSARVTDWRGLVVDTKQELSQCVALSEIEKKWKEELDQELERKRQEIMRQAGLGSSPRDRGMSRQREKSRFASPGK | ||||||
Cross-link | 109 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Cross-link | 111 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Expressed in roots, inflorescence stems, flower buds and flowers. Low amount in rosette and cauline leaves.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain, coiled coil, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 69-331 | Protein kinase | ||||
Sequence: WRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFV | ||||||
Coiled coil | 101-131 | |||||
Sequence: LIAANFASKEKTQAHIQELEEEVKLLKNLSH | ||||||
Region | 452-481 | Disordered | ||||
Sequence: KFDESPGNGEKESTMSMECDQPSYSEDDDE | ||||||
Region | 536-592 | Disordered | ||||
Sequence: GFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSRELNINNGGDEASQDGVS | ||||||
Compositional bias | 550-587 | Polar residues | ||||
Sequence: GPLGGSPSRATDATSCSKSPGSGGSRELNINNGGDEAS | ||||||
Coiled coil | 620-643 | |||||
Sequence: KKWKEELDQELERKRQEIMRQAGL | ||||||
Region | 635-666 | Disordered | ||||
Sequence: QEIMRQAGLGSSPRDRGMSRQREKSRFASPGK | ||||||
Compositional bias | 650-666 | Basic and acidic residues | ||||
Sequence: RGMSRQREKSRFASPGK |
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
O22040-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1L
- Length666
- Mass (Da)73,470
- Last updated2004-04-26 v2
- Checksum588C6339CDC58C18
O22040-2
- Name1S
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8AWJ5 | A0A1P8AWJ5_ARATH | NP1 | 620 |
Sequence caution
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_010124 | 372-376 | in isoform 1S | |||
Sequence: VGDMC → MMRIS | ||||||
Alternative sequence | VSP_010125 | 377-666 | in isoform 1S | |||
Sequence: Missing | ||||||
Compositional bias | 550-587 | Polar residues | ||||
Sequence: GPLGGSPSRATDATSCSKSPGSGGSRELNINNGGDEAS | ||||||
Compositional bias | 650-666 | Basic and acidic residues | ||||
Sequence: RGMSRQREKSRFASPGK |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB000796 EMBL· GenBank· DDBJ | BAA21854.1 EMBL· GenBank· DDBJ | mRNA | ||
AB000797 EMBL· GenBank· DDBJ | BAA21855.1 EMBL· GenBank· DDBJ | mRNA | ||
AC000106 EMBL· GenBank· DDBJ | AAB70419.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE28381.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK117282 EMBL· GenBank· DDBJ | BAC41954.1 EMBL· GenBank· DDBJ | mRNA | ||
BT005949 EMBL· GenBank· DDBJ | AAO64884.1 EMBL· GenBank· DDBJ | mRNA |