O18333 · RAB2_DROME

Function

function

May be involved in bidirectional endoplasmic reticulum (ER) to Golgi trafficking (PubMed:33822845).
Together with Rab7 involved in promoting fusion of autophagosomes and endosomes with lysosomes, probably through recruitment of the HOPS tethering complex (PubMed:28063257, PubMed:28483915, PubMed:31194677, PubMed:33822845).
Involved in biosynthetic transport to lysosomes (PubMed:28483915).
In larval motor neurons, mediates the biogenesis of presynaptic cargo vesicles and their long-range axonal trafficking to synaptic termini (PubMed:33822845).
Not involved in axonal trafficking of mitochondria (PubMed:33822845).
During vesicle biogenesis, active zone proteins (including brp/Bruchpilot) and synaptic vesicle proteins (including VGlut) are sorted from the trans-Golgi in a Rab2-dependent manner via, at least, two independent routes (PubMed:33822845).
Acts upstream of Arl8 during presynaptic precursor vesicle biogenesis (PubMed:33822845).
Associated with lysosomal marker positive presynaptic cargo vesicles during anterograde and retrograde axonal trafficking, probably while in its GTP-bound active state (PubMed:33822845).
Involved in the delivery of presynaptic cargos, but not presynapse assembly or active zone function at synaptic termini (PubMed:33822845).
Required for autophagocytosis-dependent remodeling of myofibrils and transverse-tubules (T-tubules) during metamorphosis (PubMed:28063257).

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site15GTP (UniProtKB | ChEBI)
Binding site16GTP (UniProtKB | ChEBI)
Binding site18GTP (UniProtKB | ChEBI)
Binding site19GTP (UniProtKB | ChEBI)
Binding site20GTP (UniProtKB | ChEBI)
Binding site20Mg2+ (UniProtKB | ChEBI)
Binding site21GTP (UniProtKB | ChEBI)
Binding site32GTP (UniProtKB | ChEBI)
Binding site33GTP (UniProtKB | ChEBI)
Binding site35GTP (UniProtKB | ChEBI)
Binding site38GTP (UniProtKB | ChEBI)
Binding site38Mg2+ (UniProtKB | ChEBI)
Binding site64GTP (UniProtKB | ChEBI)
Binding site119GTP (UniProtKB | ChEBI)
Binding site122GTP (UniProtKB | ChEBI)
Binding site150GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentautolysosome membrane
Cellular Componentautophagosome membrane
Cellular Componentcytoplasmic vesicle
Cellular Componentendoplasmic reticulum exit site
Cellular ComponentGolgi apparatus
Cellular ComponentGolgi membrane
Cellular Componentneuronal cell body
Cellular Componentperikaryon
Cellular Componentplasma membrane
Cellular Componentpostsynapse of neuromuscular junction
Cellular Componentpresynaptic active zone
Cellular Componenttype II terminal bouton
Cellular Componentvesicle
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionmagnesium ion binding
Biological Processautophagosome-lysosome fusion
Biological Processendoplasmic reticulum to Golgi vesicle-mediated transport
Biological Processeye pigment granule organization
Biological Processprotein secretion
Biological ProcessRab protein signal transduction
Biological Processregulation of glutamate receptor clustering
Biological Processregulation of postsynapse organization
Biological Processsecretory granule-lysosome fusion
Biological Processsymbiont entry into host cell
Biological ProcessT-tubule organization
Biological Processvesicle-mediated transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ras-related protein Rab-2
  • EC number

Gene names

    • Name
      Rab2
    • ORF names
      CG3269

Organism names

  • Taxonomic identifier
  • Strains
    • Oregon-R
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    O18333
  • Secondary accessions
    • Q9U5D5

Proteomes

Organism-specific databases

Subcellular Location

Vesicle
Cytoplasmic vesicle
Cell projection, axon
Presynapse
Golgi apparatus
Perikaryon
Cytoplasmic vesicle, autophagosome membrane
; Lipid-anchor
Autolysosome membrane
; Lipid-anchor
Note: Autophagosome localization is not dependent on Rab7, Syx17 or Vps39 (PubMed:28063257).
Associated with autolysosomes in its active GTP-bound form (PubMed:28483915).

Keywords

Phenotypes & Variants

Disruption phenotype

Lethal from late second/early third instar larval stage (PubMed:28483915, PubMed:33822845).
In the cortex of larval ventral nerve cords, disruption of axonal trafficking in motor neurons of presynaptic cargo, but not mitochondrial cargo (PubMed:33822845).
Aggregation of presynaptic active zone proteins (e.g. brp/Bruchpilot and Rbp/RIM-binding protein), synaptic vesicle proteins (e.g. unc-13, Syt1/Synaptotagmin 1 and Dap160/intersectin) and lysosomal cargos (e.g. Lamp1) in the soma of motor neurons (PubMed:33822845).
Synaptic vesicle proteins and presynaptic active zone proteins accumulate in closely apposed but separate compartments (PubMed:33822845).
These aggregates correspond to vesicles accumulating at the trans-Golgi (PubMed:33822845).
Reduced levels of active zone and synaptic vesicle proteins in synaptic termini of motor neurons (PubMed:33822845).
Thin motor neuron synaptic terminal morphology with atypically small bouton structures (PubMed:33822845).
Reduced number of active zones resulting in restricted neurotransmitter release (PubMed:33822845).
No reduction in active zone size or function, suggesting presynapse assembly is not affected (PubMed:33822845).
Fragmentation of the Golgi apparatus (PubMed:33822845).
Sorting and membrane targeting of non-synaptic proteins from the Golgi is not affected (PubMed:33822845).
Increased incidence and decreased size of autophagic organelles with decreased autophagosome-lysosome fusion in larval fat cells and reduced basal autophagic degradation (PubMed:28483915, PubMed:33822845).
Conditional RNAi-mediated knock-down in muscle cells disrupts transverse-tubule (T-tubule) and myofibril remodeling in internal oblique muscles during metamorphosis from 12 to 18 hours after pupal formation, with an accumulation of nondegraded autophagosomes and enlarged amphisomes, probably due to disruption of autophagosome-lysosome fusion (PubMed:28063257).
Conditional RNAi mediated knock-down in third instar larval fat body cells resulted in accumulation of late stage autophagosomes and nondegraded autolysosomes (PubMed:28063257).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis65Protein is locked into the activated GTP-bound state.

PTM/Processing

Features

Showing features for chain, lipidation.

TypeIDPosition(s)Description
ChainPRO_00004588971-213
Lipidation212S-geranylgeranyl cysteine
Lipidation213S-geranylgeranyl cysteine

Keywords

Proteomic databases

Expression

Developmental stage

Motor neurons of larval ventral nerve cords.

Gene expression databases

Interaction

Subunit

Interacts (GTP-bound form) with Vps16A and Vps39; the interaction with Vps39 is probably direct.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY O18333TBC1D16 Q9VKV63EBI-91903, EBI-164712

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region190-213Disordered

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    213
  • Mass (Da)
    23,561
  • Last updated
    1998-01-01 v1
  • Checksum
    61029D8EEFE7CC02
MSYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQEAFRSITRSYYRGAAGALLVYDITRRETFNHLTTWLEDARQHSNSNMVIMLIGNKSDLDSRREVKKEEGEAFAREHGLVFMETSARTAANVEEAFINTAKEIYEKIQEGVFDINNEANGIKIGQQHSPTNPSLPGAGGAAGAANSGCC

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict141in Ref. 2; BAA87878

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D84313
EMBL· GenBank· DDBJ
BAA21706.1
EMBL· GenBank· DDBJ
mRNA
AB035352
EMBL· GenBank· DDBJ
BAA87878.1
EMBL· GenBank· DDBJ
mRNA
AE013599
EMBL· GenBank· DDBJ
AAM70817.1
EMBL· GenBank· DDBJ
Genomic DNA
AE013599
EMBL· GenBank· DDBJ
AGB93265.1
EMBL· GenBank· DDBJ
Genomic DNA
BT003315
EMBL· GenBank· DDBJ
AAO25075.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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