O18333 · RAB2_DROME
- ProteinRas-related protein Rab-2
- GeneRab2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids213 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
May be involved in bidirectional endoplasmic reticulum (ER) to Golgi trafficking (PubMed:33822845).
Together with Rab7 involved in promoting fusion of autophagosomes and endosomes with lysosomes, probably through recruitment of the HOPS tethering complex (PubMed:28063257, PubMed:28483915, PubMed:31194677, PubMed:33822845).
Involved in biosynthetic transport to lysosomes (PubMed:28483915).
In larval motor neurons, mediates the biogenesis of presynaptic cargo vesicles and their long-range axonal trafficking to synaptic termini (PubMed:33822845).
Not involved in axonal trafficking of mitochondria (PubMed:33822845).
During vesicle biogenesis, active zone proteins (including brp/Bruchpilot) and synaptic vesicle proteins (including VGlut) are sorted from the trans-Golgi in a Rab2-dependent manner via, at least, two independent routes (PubMed:33822845).
Acts upstream of Arl8 during presynaptic precursor vesicle biogenesis (PubMed:33822845).
Associated with lysosomal marker positive presynaptic cargo vesicles during anterograde and retrograde axonal trafficking, probably while in its GTP-bound active state (PubMed:33822845).
Involved in the delivery of presynaptic cargos, but not presynapse assembly or active zone function at synaptic termini (PubMed:33822845).
Required for autophagocytosis-dependent remodeling of myofibrils and transverse-tubules (T-tubules) during metamorphosis (PubMed:28063257).
Together with Rab7 involved in promoting fusion of autophagosomes and endosomes with lysosomes, probably through recruitment of the HOPS tethering complex (PubMed:28063257, PubMed:28483915, PubMed:31194677, PubMed:33822845).
Involved in biosynthetic transport to lysosomes (PubMed:28483915).
In larval motor neurons, mediates the biogenesis of presynaptic cargo vesicles and their long-range axonal trafficking to synaptic termini (PubMed:33822845).
Not involved in axonal trafficking of mitochondria (PubMed:33822845).
During vesicle biogenesis, active zone proteins (including brp/Bruchpilot) and synaptic vesicle proteins (including VGlut) are sorted from the trans-Golgi in a Rab2-dependent manner via, at least, two independent routes (PubMed:33822845).
Acts upstream of Arl8 during presynaptic precursor vesicle biogenesis (PubMed:33822845).
Associated with lysosomal marker positive presynaptic cargo vesicles during anterograde and retrograde axonal trafficking, probably while in its GTP-bound active state (PubMed:33822845).
Involved in the delivery of presynaptic cargos, but not presynapse assembly or active zone function at synaptic termini (PubMed:33822845).
Required for autophagocytosis-dependent remodeling of myofibrils and transverse-tubules (T-tubules) during metamorphosis (PubMed:28063257).
Catalytic activity
Ras-related protein Rab-2
GTP + H2O = GDP + H+ + phosphate
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 15 | GTP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 16 | GTP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 18 | GTP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 19 | GTP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 20 | GTP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 20 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 21 | GTP (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 32 | GTP (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 33 | GTP (UniProtKB | ChEBI) | ||||
Sequence: P | ||||||
Binding site | 35 | GTP (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 38 | GTP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 38 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 64 | GTP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 119 | GTP (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 122 | GTP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 150 | GTP (UniProtKB | ChEBI) | ||||
Sequence: A |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRas-related protein Rab-2
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionO18333
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor
Autolysosome membrane ; Lipid-anchor
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Lethal from late second/early third instar larval stage (PubMed:28483915, PubMed:33822845).
In the cortex of larval ventral nerve cords, disruption of axonal trafficking in motor neurons of presynaptic cargo, but not mitochondrial cargo (PubMed:33822845).
Aggregation of presynaptic active zone proteins (e.g. brp/Bruchpilot and Rbp/RIM-binding protein), synaptic vesicle proteins (e.g. unc-13, Syt1/Synaptotagmin 1 and Dap160/intersectin) and lysosomal cargos (e.g. Lamp1) in the soma of motor neurons (PubMed:33822845).
Synaptic vesicle proteins and presynaptic active zone proteins accumulate in closely apposed but separate compartments (PubMed:33822845).
These aggregates correspond to vesicles accumulating at the trans-Golgi (PubMed:33822845).
Reduced levels of active zone and synaptic vesicle proteins in synaptic termini of motor neurons (PubMed:33822845).
Thin motor neuron synaptic terminal morphology with atypically small bouton structures (PubMed:33822845).
Reduced number of active zones resulting in restricted neurotransmitter release (PubMed:33822845).
No reduction in active zone size or function, suggesting presynapse assembly is not affected (PubMed:33822845).
Fragmentation of the Golgi apparatus (PubMed:33822845).
Sorting and membrane targeting of non-synaptic proteins from the Golgi is not affected (PubMed:33822845).
Increased incidence and decreased size of autophagic organelles with decreased autophagosome-lysosome fusion in larval fat cells and reduced basal autophagic degradation (PubMed:28483915, PubMed:33822845).
Conditional RNAi-mediated knock-down in muscle cells disrupts transverse-tubule (T-tubule) and myofibril remodeling in internal oblique muscles during metamorphosis from 12 to 18 hours after pupal formation, with an accumulation of nondegraded autophagosomes and enlarged amphisomes, probably due to disruption of autophagosome-lysosome fusion (PubMed:28063257).
Conditional RNAi mediated knock-down in third instar larval fat body cells resulted in accumulation of late stage autophagosomes and nondegraded autolysosomes (PubMed:28063257).
In the cortex of larval ventral nerve cords, disruption of axonal trafficking in motor neurons of presynaptic cargo, but not mitochondrial cargo (PubMed:33822845).
Aggregation of presynaptic active zone proteins (e.g. brp/Bruchpilot and Rbp/RIM-binding protein), synaptic vesicle proteins (e.g. unc-13, Syt1/Synaptotagmin 1 and Dap160/intersectin) and lysosomal cargos (e.g. Lamp1) in the soma of motor neurons (PubMed:33822845).
Synaptic vesicle proteins and presynaptic active zone proteins accumulate in closely apposed but separate compartments (PubMed:33822845).
These aggregates correspond to vesicles accumulating at the trans-Golgi (PubMed:33822845).
Reduced levels of active zone and synaptic vesicle proteins in synaptic termini of motor neurons (PubMed:33822845).
Thin motor neuron synaptic terminal morphology with atypically small bouton structures (PubMed:33822845).
Reduced number of active zones resulting in restricted neurotransmitter release (PubMed:33822845).
No reduction in active zone size or function, suggesting presynapse assembly is not affected (PubMed:33822845).
Fragmentation of the Golgi apparatus (PubMed:33822845).
Sorting and membrane targeting of non-synaptic proteins from the Golgi is not affected (PubMed:33822845).
Increased incidence and decreased size of autophagic organelles with decreased autophagosome-lysosome fusion in larval fat cells and reduced basal autophagic degradation (PubMed:28483915, PubMed:33822845).
Conditional RNAi-mediated knock-down in muscle cells disrupts transverse-tubule (T-tubule) and myofibril remodeling in internal oblique muscles during metamorphosis from 12 to 18 hours after pupal formation, with an accumulation of nondegraded autophagosomes and enlarged amphisomes, probably due to disruption of autophagosome-lysosome fusion (PubMed:28063257).
Conditional RNAi mediated knock-down in third instar larval fat body cells resulted in accumulation of late stage autophagosomes and nondegraded autolysosomes (PubMed:28063257).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 65 | Protein is locked into the activated GTP-bound state. | ||||
Sequence: Q → L |
PTM/Processing
Features
Showing features for chain, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000458897 | 1-213 | Ras-related protein Rab-2 | |||
Sequence: MSYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQEAFRSITRSYYRGAAGALLVYDITRRETFNHLTTWLEDARQHSNSNMVIMLIGNKSDLDSRREVKKEEGEAFAREHGLVFMETSARTAANVEEAFINTAKEIYEKIQEGVFDINNEANGIKIGQQHSPTNPSLPGAGGAAGAANSGCC | ||||||
Lipidation | 212 | S-geranylgeranyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 213 | S-geranylgeranyl cysteine | ||||
Sequence: C |
Keywords
- PTM
Proteomic databases
Expression
Developmental stage
Motor neurons of larval ventral nerve cords.
Gene expression databases
Interaction
Subunit
Interacts (GTP-bound form) with Vps16A and Vps39; the interaction with Vps39 is probably direct.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | O18333 | TBC1D16 Q9VKV6 | 3 | EBI-91903, EBI-164712 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 190-213 | Disordered | ||||
Sequence: QHSPTNPSLPGAGGAAGAANSGCC |
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length213
- Mass (Da)23,561
- Last updated1998-01-01 v1
- Checksum61029D8EEFE7CC02
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 141 | in Ref. 2; BAA87878 | ||||
Sequence: H → N |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D84313 EMBL· GenBank· DDBJ | BAA21706.1 EMBL· GenBank· DDBJ | mRNA | ||
AB035352 EMBL· GenBank· DDBJ | BAA87878.1 EMBL· GenBank· DDBJ | mRNA | ||
AE013599 EMBL· GenBank· DDBJ | AAM70817.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE013599 EMBL· GenBank· DDBJ | AGB93265.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT003315 EMBL· GenBank· DDBJ | AAO25075.1 EMBL· GenBank· DDBJ | mRNA |