O17608 · SLFL1_CAEEL

  • Protein
    Schlafen-like protein 1
  • Gene
    tofu-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Component of the trimeric PUCH (precursor of 21U RNA 5'-end cleavage holoenzyme) complex, that acts as an endoribonuclease processing the 5'-end of precursor Piwi-interacting RNAs (piRNAs) (PubMed:37758951).
The PUCH complex consists of tofu-1, tofu-2 and either slfl-3 or slfl-4, with tofu-2 exhibiting endoribonuclease activity (PubMed:37758951).
PUCH-mediated processing strictly requires a 7-methyl-G cap (m7 G-cap) and an uracil at position three (U3) (PubMed:37758951).
PUCH also exhibits a strict bias for piRNA precursors with an A or G at position 1 (PubMed:37758951).
Mature piRNA production is enhanced by the interaction of PUCH with the PETISCO complex, which is stabilizing piRNA precursors and allows their processing by PUCH (PubMed:37758951).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentendoribonuclease complex
Biological Processprimary piRNA processing

Names & Taxonomy

Protein names

  • Recommended name
    Schlafen-like protein 1
  • Short names
    SLFL-1
    ; SLFN-like 1
  • Alternative names
    • 21U-RNA biogenesis fouled up protein 1
    • PUCH complex member slfl-1
    • Protein tofu-1

Gene names

    • Name
      tofu-1
    • ORF names
      C27H6.3

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    O17608

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004524671-373Schlafen-like protein 1

Proteomic databases

Expression

Tissue specificity

Expressed in the germline.

Gene expression databases

Interaction

Subunit

Component of the trimeric PUCH (precursor of 21U RNA 5'-end cleavage holoenzyme) complex; consisting of tofu-1, tofu-2 and either slfl-3 or slfl-4; which is required for processing of piRNA precursors (PubMed:37758951).
Within the complex, interacts (via N-terminus) with tofu-2 (via N-terminus); the interaction stabilizes tofu-2 and may form a functional nuclease (PubMed:37758951).
Within the complex, required for the interaction of tofu-2 (via N-terminus) with slfl-3 (via N-terminus) (PubMed:37758951).
Interacts (via residues 82-172) with the PETISCO complex subunit tofu-6 (via residues 120-314); the interaction between the PETISCO and PUCH complex members enhances piRNA production in vivo (PubMed:37758951).
View interactors in UniProtKB
View CPX-8565 in Complex Portal

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region1-67Disordered
Compositional bias10-67Acidic residues
Region247-373SLFN-like fold

Domain

In mammalian Schlafen proteins, two SLFN-folds come together to form the nuclease domain. In tofu-1 and tofu-2, only one SLFN-like fold can be identified, suggesting that the two proteins may interact via their SLFN-like folds to form a functional nuclease.

Sequence similarities

Belongs to the Schlafen family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    373
  • Mass (Da)
    41,984
  • Last updated
    2008-02-05 v3
  • Checksum
    2E2C003672F0FD5B
MAALFEENDSYSDSENEEDFSGFLSEIEENENEDEFENDDEFEDDDELENVDELEDDELSSEEVDIPSENEVIVELHDGQAEVEESLLDALLGKALAGDQMNSRIEGLFEDLDSSVEPDIIDRSELEDVDSGISVGAVSSSSAETQASDAKCKNKDAFDMSSLKKYMQYITEEEHAVSVEDIPDFASKKNVYGTSFNVRNGIMSRPPRFEFARHDQRWIDSCVSMAYEKDKTLKELITLGKLTTTVKAGAKIEFRTKFTDVHIRSADEINLMETHDIQQIIRDALNSKRLAVVLFGIGKDGKVTGCHMGPGQQDNLRLALDTAVQTEFAPPIENILDVVDMNFVPVEEMDETFLIVIRVKQLRNQVYRLESSV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias10-67Acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BX284605
EMBL· GenBank· DDBJ
CAB02794.3
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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