O17386 · CED8_CAEEL
- ProteinCell death abnormality protein 8
- Geneced-8
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids458 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Phospholipid scramblase that acts downstream of ced-9 and caspase ced-3 to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:24225442).
Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment (PubMed:23845944, PubMed:24225442).
Regulates apoptosis kinetics during embryonic development (PubMed:10882128, PubMed:1936965, PubMed:23845944, PubMed:24225442).
Not required for engulfment of germ cell corpses (PubMed:9927601).
Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment (PubMed:23845944, PubMed:24225442).
Regulates apoptosis kinetics during embryonic development (PubMed:10882128, PubMed:1936965, PubMed:23845944, PubMed:24225442).
Not required for engulfment of germ cell corpses (PubMed:9927601).
Catalytic activity
- a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)
Features
Showing features for site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Site | 21-22 | Cleavage; by ced-3 | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | phospholipid scramblase activity | |
Biological Process | apoptotic process involved in development | |
Biological Process | embryo development ending in birth or egg hatching | |
Biological Process | engulfment of apoptotic cell | |
Biological Process | execution phase of apoptosis | |
Biological Process | phosphatidylserine exposure on apoptotic cell surface | |
Biological Process | programmed cell death |
Keywords
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCell death abnormality protein 8
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionO17386
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-45 | Cytoplasmic | |||
Transmembrane | 46-66 | Helical | |||
Topological domain | 67-77 | Extracellular | |||
Transmembrane | 78-98 | Helical | |||
Topological domain | 99-123 | Cytoplasmic | |||
Transmembrane | 124-144 | Helical | |||
Topological domain | 145-219 | Extracellular | |||
Transmembrane | 220-240 | Helical | |||
Topological domain | 241-274 | Cytoplasmic | |||
Transmembrane | 275-295 | Helical | |||
Transmembrane | 296-316 | Helical | |||
Topological domain | 317 | Extracellular | |||
Transmembrane | 318-338 | Helical | |||
Topological domain | 339-353 | Cytoplasmic | |||
Transmembrane | 354-374 | Helical | |||
Topological domain | 375-378 | Extracellular | |||
Transmembrane | 379-399 | Helical | |||
Topological domain | 400-458 | Cytoplasmic | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Delayed cell death process.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 1-22 | Causes constitutive phosphatidylserine cell surface exposure. | |||
Mutagenesis | 21 | Loss of ced-3-mediated cleavage. | |||
Mutagenesis | 21 | Loss of ced-3-mediated cleavage. Reduced phosphatidylserine cell surface exposure in apoptotic cells. Reduced number of cell corpses at the comma stage followed by an increase at the 4-fold stage. | |||
Mutagenesis | 163 | No defect in apoptosis during embryogenesis. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000379936 | 1-458 | Cell death abnormality protein 8 | ||
Post-translational modification
Cleavage by ced-3 activates ced-8 function in promoting phosphatidylserine exposure at the surface of apoptotic cells.
Proteomic databases
Expression
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length458
- Mass (Da)53,273
- Last updated2003-10-01 v2
- Checksum9E04EEBB0299DB2C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FO081089 EMBL· GenBank· DDBJ | CCD69028.1 EMBL· GenBank· DDBJ | Genomic DNA |