O15743 · SPNA_DICDI

Function

function

Involved in cell-type differentiation and morphogenesis. Dephosphorylates casein; in vitro. May also be involved as modulators or transducers in various transmembrane signaling systems.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 magnesium or manganese ions per subunit.

Activity regulation

Inhibited by 50 mM NaF (sodium fluoride).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site122-129GTP (UniProtKB | ChEBI)
Binding site261-267GTP (UniProtKB | ChEBI)
Binding site286-290GTP (UniProtKB | ChEBI)
Binding site373-376GTP (UniProtKB | ChEBI)
Binding site749Mn2+ 1 (UniProtKB | ChEBI)
Binding site750Mn2+ 1 (UniProtKB | ChEBI)
Binding site920Mn2+ 2 (UniProtKB | ChEBI)
Binding site963Mn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmembrane
Cellular Componentplasma membrane
Cellular Componentruffle
Molecular FunctionG-protein beta/gamma-subunit complex binding
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionmagnesium ion binding
Molecular Functionmyosin phosphatase activity
Molecular Functionprotein serine/threonine phosphatase activity
Biological Processanatomical structure morphogenesis
Biological Processcell differentiation
Biological ProcessG protein-coupled receptor signaling pathway

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Protein spalten

Including 2 domains:

  • Recommended name
    Probable guanine nucleotide-binding protein spalten
  • Recommended name
    Protein serine/threonine phosphatase spalten
  • EC number

Gene names

    • Name
      spnA
    • Synonyms
      spn
    • ORF names
      DDB_G0276155

Organism names

Accessions

  • Primary accession
    O15743
  • Secondary accessions
    • Q552E7

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Morphological arrest at the mound stage and a defect in cell-type differentiation. Instead of going through morphogenesis, after 16 hours of development, the mounds disaggregate to form smaller aggregates that eventually produce abnormal looking finger-like structures.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis373Leads to the formation of very small-sized fruiting bodies.
Mutagenesis376Leads to the formation of abnormal looking fruiting bodies.
Mutagenesis920Loss of phosphatase activity; when associated with A-924.
Mutagenesis924Loss of phosphatase activity; when associated with A-920.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003675751-975Protein spalten

Proteomic databases

Expression

Developmental stage

Moderately expressed during growth. Increases during development, peaking at around 8 hours of development (mound stage) and then decreases gradually during the later stages. Mainly expressed in the prestalk cell population during the later multicellular stages. Required for prestalk specific gene expression and for prespore cell differentiation. Expressed (at protein level).

Interaction

Subunit

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, coiled coil, domain, compositional bias.

TypeIDPosition(s)Description
Region1-31Disordered
Coiled coil21-70
Region64-99Disordered
Domain114-458G-alpha
Region117-130G1 motif
Region259-267G2 motif
Region282-291G3 motif
Region369-376G4 motif
Region427-432G5 motif
Region455-520Disordered
Compositional bias541-589Polar residues
Region541-700Disordered
Compositional bias596-684Basic and acidic residues
Domain704-972PPM-type phosphatase

Sequence similarities

In the N-terminal section; belongs to the G-alpha family.
In the C-terminal section; belongs to the PP2C family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    975
  • Mass (Da)
    109,016
  • Last updated
    1998-01-01 v1
  • Checksum
    BBB322F5026D2A4F
MKKMLFMNKKEKKEEQSPAHSSLAQQHQLAQQQYQLQQQQLQLQYQQHQQQLQLAQQQKQNEQNLAQLSTSTSSNSSVNNTTNTNTNTTNSSSISSNNNNNNNTAVPILKAHDFCGTIMILGHTESGKTTLQRQLEFIYGVTDPTDAKHYQRLIYGNTLATLIRFIENSERLNITLSPDNLARVKRIQSQPVELARNRLPRFPLKLGWDCKCIWEDKVIQSVYNHSKICSEIRTPGRPKYYMDRMFKVFDPSYTPTEMDIISAYDQKDTIQSSAIIHKRFKVDLFGCSGKQSSPKNWVGLHQNYKPNYIFYVVALKDYFSDHLVATQNTDPTIVEMCNNHIHRNLLLESLNSFETLTKSELFDKSLAIYLIFNTSDIFYENIKQYDLKSCFSEYEGGNDPEKAVSFISNKFTKFLQNKDKPYKSHIVNLLDKNNVREEFEGIFDSLKIDAEKRGFTTPYNQSNSSPVSSIGSNSSRNSRLPNTSVSIPGLYSSDNDNTRLKNVNNNNNNNNNTTTYGSSTFPSSVISTTGSISNSIASAMDNDSSYSNESSPTSSMTLLPTTTTTTTTTTTTATTTDSTNNNNNNATVVIGKGKPPKEPKPVKPPKEPKPPKEPKPPKEPKPPKEPKPIKEPKEKPVKESKPPKEPKPIKEPKESKEPKEPKEPKPTKPPKEKKTSKVDGAAESKKNGADSCGNGGVGSKIKLESGFGSLQGRRKNMEDTHVILNNLMGAVTYNGPPKDIPISYYAVYDGHGGTETSTLLEPTVHNCLVNSQSFRDGDYEQAFRDAYAEADDIVIEKCEKSGSTGVSALLVGNKLYTANVGDSEIVLARAQPNANPKGPVTYEPVLLSYKHLASDDQEKKRVTDLGGMIIFNRLFGSLAVSRSFGDKEYKEGEKKFCVSDPYQTTTDLTARDHFFILACDGLWDKVEYDEAVQFVQRNIKLGKSATEISELLAQDSYDRGSGDNITVLVVILNWN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias541-589Polar residues
Compositional bias596-684Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF019985
EMBL· GenBank· DDBJ
AAB70844.1
EMBL· GenBank· DDBJ
mRNA
AAFI02000014
EMBL· GenBank· DDBJ
EAL69377.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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