O15743 · SPNA_DICDI
- ProteinProtein spalten
- GenespnA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids975 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in cell-type differentiation and morphogenesis. Dephosphorylates casein; in vitro. May also be involved as modulators or transducers in various transmembrane signaling systems.
Catalytic activity
- H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] + phosphate
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 2 magnesium or manganese ions per subunit.
Activity regulation
Inhibited by 50 mM NaF (sodium fluoride).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 122-129 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GHTESGKT | ||||||
Binding site | 261-267 | GTP (UniProtKB | ChEBI) | ||||
Sequence: ISAYDQK | ||||||
Binding site | 286-290 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GCSGK | ||||||
Binding site | 373-376 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NTSD | ||||||
Binding site | 749 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 750 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 920 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 963 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Cellular Component | ruffle | |
Molecular Function | G-protein beta/gamma-subunit complex binding | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | myosin phosphatase activity | |
Molecular Function | protein serine/threonine phosphatase activity | |
Biological Process | anatomical structure morphogenesis | |
Biological Process | cell differentiation | |
Biological Process | G protein-coupled receptor signaling pathway |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameProtein spalten
Including 2 domains:
- Recommended nameProbable guanine nucleotide-binding protein spalten
- Recommended nameProtein serine/threonine phosphatase spalten
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Amoebozoa > Evosea > Eumycetozoa > Dictyostelia > Dictyosteliales > Dictyosteliaceae > Dictyostelium
Accessions
- Primary accessionO15743
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Morphological arrest at the mound stage and a defect in cell-type differentiation. Instead of going through morphogenesis, after 16 hours of development, the mounds disaggregate to form smaller aggregates that eventually produce abnormal looking finger-like structures.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 373 | Leads to the formation of very small-sized fruiting bodies. | ||||
Sequence: N → D | ||||||
Mutagenesis | 376 | Leads to the formation of abnormal looking fruiting bodies. | ||||
Sequence: D → A | ||||||
Mutagenesis | 920 | Loss of phosphatase activity; when associated with A-924. | ||||
Sequence: D → A | ||||||
Mutagenesis | 924 | Loss of phosphatase activity; when associated with A-920. | ||||
Sequence: D → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000367575 | 1-975 | Protein spalten | |||
Sequence: MKKMLFMNKKEKKEEQSPAHSSLAQQHQLAQQQYQLQQQQLQLQYQQHQQQLQLAQQQKQNEQNLAQLSTSTSSNSSVNNTTNTNTNTTNSSSISSNNNNNNNTAVPILKAHDFCGTIMILGHTESGKTTLQRQLEFIYGVTDPTDAKHYQRLIYGNTLATLIRFIENSERLNITLSPDNLARVKRIQSQPVELARNRLPRFPLKLGWDCKCIWEDKVIQSVYNHSKICSEIRTPGRPKYYMDRMFKVFDPSYTPTEMDIISAYDQKDTIQSSAIIHKRFKVDLFGCSGKQSSPKNWVGLHQNYKPNYIFYVVALKDYFSDHLVATQNTDPTIVEMCNNHIHRNLLLESLNSFETLTKSELFDKSLAIYLIFNTSDIFYENIKQYDLKSCFSEYEGGNDPEKAVSFISNKFTKFLQNKDKPYKSHIVNLLDKNNVREEFEGIFDSLKIDAEKRGFTTPYNQSNSSPVSSIGSNSSRNSRLPNTSVSIPGLYSSDNDNTRLKNVNNNNNNNNNTTTYGSSTFPSSVISTTGSISNSIASAMDNDSSYSNESSPTSSMTLLPTTTTTTTTTTTTATTTDSTNNNNNNATVVIGKGKPPKEPKPVKPPKEPKPPKEPKPPKEPKPPKEPKPIKEPKEKPVKESKPPKEPKPIKEPKESKEPKEPKEPKPTKPPKEKKTSKVDGAAESKKNGADSCGNGGVGSKIKLESGFGSLQGRRKNMEDTHVILNNLMGAVTYNGPPKDIPISYYAVYDGHGGTETSTLLEPTVHNCLVNSQSFRDGDYEQAFRDAYAEADDIVIEKCEKSGSTGVSALLVGNKLYTANVGDSEIVLARAQPNANPKGPVTYEPVLLSYKHLASDDQEKKRVTDLGGMIIFNRLFGSLAVSRSFGDKEYKEGEKKFCVSDPYQTTTDLTARDHFFILACDGLWDKVEYDEAVQFVQRNIKLGKSATEISELLAQDSYDRGSGDNITVLVVILNWN |
Proteomic databases
Expression
Developmental stage
Moderately expressed during growth. Increases during development, peaking at around 8 hours of development (mound stage) and then decreases gradually during the later stages. Mainly expressed in the prestalk cell population during the later multicellular stages. Required for prestalk specific gene expression and for prespore cell differentiation. Expressed (at protein level).
Interaction
Subunit
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, coiled coil, domain, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-31 | Disordered | ||||
Sequence: MKKMLFMNKKEKKEEQSPAHSSLAQQHQLAQ | ||||||
Coiled coil | 21-70 | |||||
Sequence: SSLAQQHQLAQQQYQLQQQQLQLQYQQHQQQLQLAQQQKQNEQNLAQLST | ||||||
Region | 64-99 | Disordered | ||||
Sequence: NLAQLSTSTSSNSSVNNTTNTNTNTTNSSSISSNNN | ||||||
Domain | 114-458 | G-alpha | ||||
Sequence: FCGTIMILGHTESGKTTLQRQLEFIYGVTDPTDAKHYQRLIYGNTLATLIRFIENSERLNITLSPDNLARVKRIQSQPVELARNRLPRFPLKLGWDCKCIWEDKVIQSVYNHSKICSEIRTPGRPKYYMDRMFKVFDPSYTPTEMDIISAYDQKDTIQSSAIIHKRFKVDLFGCSGKQSSPKNWVGLHQNYKPNYIFYVVALKDYFSDHLVATQNTDPTIVEMCNNHIHRNLLLESLNSFETLTKSELFDKSLAIYLIFNTSDIFYENIKQYDLKSCFSEYEGGNDPEKAVSFISNKFTKFLQNKDKPYKSHIVNLLDKNNVREEFEGIFDSLKIDAEKRGFTTP | ||||||
Region | 117-130 | G1 motif | ||||
Sequence: TIMILGHTESGKTT | ||||||
Region | 259-267 | G2 motif | ||||
Sequence: DIISAYDQK | ||||||
Region | 282-291 | G3 motif | ||||
Sequence: VDLFGCSGKQ | ||||||
Region | 369-376 | G4 motif | ||||
Sequence: YLIFNTSD | ||||||
Region | 427-432 | G5 motif | ||||
Sequence: VNLLDK | ||||||
Region | 455-520 | Disordered | ||||
Sequence: FTTPYNQSNSSPVSSIGSNSSRNSRLPNTSVSIPGLYSSDNDNTRLKNVNNNNNNNNNTTTYGSST | ||||||
Compositional bias | 541-589 | Polar residues | ||||
Sequence: DNDSSYSNESSPTSSMTLLPTTTTTTTTTTTTATTTDSTNNNNNNATVV | ||||||
Region | 541-700 | Disordered | ||||
Sequence: DNDSSYSNESSPTSSMTLLPTTTTTTTTTTTTATTTDSTNNNNNNATVVIGKGKPPKEPKPVKPPKEPKPPKEPKPPKEPKPPKEPKPIKEPKEKPVKESKPPKEPKPIKEPKESKEPKEPKEPKPTKPPKEKKTSKVDGAAESKKNGADSCGNGGVGSK | ||||||
Compositional bias | 596-684 | Basic and acidic residues | ||||
Sequence: PKEPKPVKPPKEPKPPKEPKPPKEPKPPKEPKPIKEPKEKPVKESKPPKEPKPIKEPKESKEPKEPKEPKPTKPPKEKKTSKVDGAAES | ||||||
Domain | 704-972 | PPM-type phosphatase | ||||
Sequence: ESGFGSLQGRRKNMEDTHVILNNLMGAVTYNGPPKDIPISYYAVYDGHGGTETSTLLEPTVHNCLVNSQSFRDGDYEQAFRDAYAEADDIVIEKCEKSGSTGVSALLVGNKLYTANVGDSEIVLARAQPNANPKGPVTYEPVLLSYKHLASDDQEKKRVTDLGGMIIFNRLFGSLAVSRSFGDKEYKEGEKKFCVSDPYQTTTDLTARDHFFILACDGLWDKVEYDEAVQFVQRNIKLGKSATEISELLAQDSYDRGSGDNITVLVVIL |
Sequence similarities
In the N-terminal section; belongs to the G-alpha family.
In the C-terminal section; belongs to the PP2C family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length975
- Mass (Da)109,016
- Last updated1998-01-01 v1
- ChecksumBBB322F5026D2A4F
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 541-589 | Polar residues | ||||
Sequence: DNDSSYSNESSPTSSMTLLPTTTTTTTTTTTTATTTDSTNNNNNNATVV | ||||||
Compositional bias | 596-684 | Basic and acidic residues | ||||
Sequence: PKEPKPVKPPKEPKPPKEPKPPKEPKPPKEPKPIKEPKEKPVKESKPPKEPKPIKEPKESKEPKEPKEPKPTKPPKEKKTSKVDGAAES |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF019985 EMBL· GenBank· DDBJ | AAB70844.1 EMBL· GenBank· DDBJ | mRNA | ||
AAFI02000014 EMBL· GenBank· DDBJ | EAL69377.1 EMBL· GenBank· DDBJ | Genomic DNA |