O14756 · H17B6_HUMAN
- Protein17-beta-hydroxysteroid dehydrogenase type 6
- GeneHSD17B6
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids317 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids.
Catalytic activity
- all-trans-retinol--[retinol-binding protein] + NAD+ = all-trans-retinal--[retinol-binding protein] + H+ + NADH
- 3alpha-hydroxy-5alpha-pregnan-20-one + NAD+ = 5alpha-pregnane-3,20-dione + H+ + NADHThis reaction proceeds in the forward direction.
- 5alpha-androstane-3beta,17beta-diol + NAD+ = 17beta-hydroxy-5alpha-androstan-3-one + H+ + NADHThis reaction proceeds in the backward direction.
- 3beta-hydroxy-5alpha-androstan-17-one + NAD+ = 5alpha-androstan-3,17-dione + H+ + NADHThis reaction proceeds in the backward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.19 μM | NAD | |||||
0.18 μM | NADH | |||||
54 μM | NADPH | |||||
940 μM | NADP | |||||
3.2 μM | all-trans-retinol | |||||
0.24 μM | allopregnanolone | |||||
0.13 μM | 3-alpha-androstanediol | |||||
0.23 μM | androsterone | |||||
0.13 μM | dehydroepiandrosterone |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
1.2 nmol/min/mg | with all-trans-retinol | ||||
14.7 nmol/min/mg | with allopregnanolone | ||||
16.5 nmol/min/mg | with 3-alpha-androstanediol | ||||
35 nmol/min/mg | with androsterone | ||||
0.9 nmol/min/mg | with dehydroepiandrosterone |
The kinetic parameters were determined using microsomes from transfected cells.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | early endosome membrane | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | intracellular membrane-bounded organelle | |
Molecular Function | 5alpha-androstane-3beta,17beta-diol dehydrogenase activity | |
Molecular Function | all-trans-retinol dehydrogenase (NAD+) activity | |
Molecular Function | androstan-3-alpha,17-beta-diol dehydrogenase activity | |
Molecular Function | androsterone dehydrogenase activity | |
Molecular Function | catalytic activity | |
Molecular Function | electron transfer activity | |
Molecular Function | estradiol 17-beta-dehydrogenase [NAD(P)+] activity | |
Molecular Function | oxidoreductase activity | |
Molecular Function | testosterone 17-beta-dehydrogenase (NADP+) activity | |
Molecular Function | testosterone dehydrogenase (NAD+) activity | |
Biological Process | androgen biosynthetic process | |
Biological Process | androgen catabolic process | |
Biological Process | brexanolone catabolic process | |
Biological Process | retinol metabolic process | |
Biological Process | steroid metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended name17-beta-hydroxysteroid dehydrogenase type 6
- EC number
- Short names17-beta-HSD 6; 17-beta-HSD6
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionO14756
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Microsome membrane ; Peripheral membrane protein
Early endosome membrane ; Peripheral membrane protein
Keywords
- Cellular component
Disease & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 410 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-17 | |||||
Sequence: MWLYLAAFVGLYYLLHW | ||||||
Chain | PRO_0000303211 | 18-317 | 17-beta-hydroxysteroid dehydrogenase type 6 | |||
Sequence: YRERQVVSHLQDKYVFITGCDSGFGNLLARQLDARGLRVLAACLTEKGAEQLRGQTSDRLETVTLDVTKMESIAAATQWVKEHVGDRGLWGLVNNAGILTPITLCEWLNTEDSMNMLKVNLIGVIQVTLSMLPLVRRARGRIVNVSSILGRVAFFVGGYCVSKYGVEAFSDILRREIQHFGVKISIVEPGYFRTGMTNMTQSLERMKQSWKEAPKHIKETYGQQYFDALYNIMKEGLLNCSTNLNLVTDCMEHALTSVHPRTRYSAGWDAKFFFIPLSYLPTSLADYILTRSWPKPAQAV | ||||||
Glycosylation | 161 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 215 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 256 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain.
Gene expression databases
Organism-specific databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length317
- Mass (Da)35,966
- Last updated1998-01-01 v1
- Checksum46F1E940605CBEE9
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 63 | in Ref. 1; AAB88252 | ||||
Sequence: E → D | ||||||
Sequence conflict | 105 | in Ref. 1; AAB88252 | ||||
Sequence: G → R |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U89281 EMBL· GenBank· DDBJ | AAB88252.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AF016509 EMBL· GenBank· DDBJ | AAB67236.1 EMBL· GenBank· DDBJ | mRNA | ||
AF223225 EMBL· GenBank· DDBJ | AAF81017.1 EMBL· GenBank· DDBJ | mRNA | ||
BC020710 EMBL· GenBank· DDBJ | AAH20710.1 EMBL· GenBank· DDBJ | mRNA |