O13979 · MU105_SCHPO

Function

function

Deubiquitinase with endodeubiquitinase activity that preferentially cleaves 'Lys-48'-linked polyubiquitin chains (PubMed:29476094, PubMed:29576528).
Shows only weak activity against 'Lys-63' and 'Lys-11'-linked chains (PubMed:29476094).
Has a role in meiosis (PubMed:16303567).

Catalytic activity

  • Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
    EC:3.4.19.12 (UniProtKB | ENZYME | Rhea)

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
12.2 μMArg-Leu-Arg-Gly-Gly-AMC
kcat is 7.2 sec-1 with Arg-Leu-Arg-Gly-Gly-AMC as substrate.

Features

Showing features for active site, site.

Type
IDPosition(s)Description
Active site42Nucleophile
Site161Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
Active site165Proton acceptor
Active site183

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functioncysteine-type deubiquitinase activity
Molecular FunctionK48-linked deubiquitinase activity
Molecular Functionubiquitin-like protein peptidase activity
Biological Processmeiotic cell cycle

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Ubiquitin carboxyl-terminal hydrolase mug105
  • EC number
  • Alternative names
    • Lys-48-selective deubiquitinase mug105 (DUB)
    • Meiotically up-regulated gene 105 protein

Gene names

    • Name
      mug105
    • ORF names
      SPAC25H1.04

Organism names

Accessions

  • Primary accession
    O13979

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis42Abolishes catalytic activity.

Variants

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The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002785041-244Ubiquitin carboxyl-terminal hydrolase mug105

Proteomic databases

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the peptidase C78 family. ZUFSP subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    244
  • Mass (Da)
    28,084
  • Last updated
    1998-01-01 v1
  • Checksum
    DE1A62DBE55BC1C7
MSKCLQQLKRQLQHFGIDGCSLADGDIDYFFTVTGIDRGWGCGWRNIQMLISWLQYTNPNWFKRNFSSGNYEINSLQSLLLSAWMKGIDAEGYAQLGDNLHGKWIGATEVYSLFTGLFVNVALVDFDFRSEASASNALFLYVKKHFESSNDTSNVSPCYLQFQGHSIIIIGFCSSLETLVVLDPDRYQSVQKKFVNIADFNHCYMRKKRSLKFSQFQLVHFKQNIFLNDFSSKLEVRSTRISDF

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CU329670
EMBL· GenBank· DDBJ
CAB11605.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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