O13597 · ERG24_SEPLY
- ProteinDelta(14)-sterol reductase
- GeneERG3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids512 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol.
Catalytic activity
- 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + H+ + NADPH
Pathway
Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 380 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 384 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 407 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 412 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 419-420 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: NY | ||||||
Binding site | 484 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 488-492 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: CRRKY | ||||||
Binding site | 499 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | delta14-sterol reductase activity | |
Molecular Function | NADP binding | |
Biological Process | ergosterol biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDelta(14)-sterol reductase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Dothideomycetidae > Mycosphaerellales > Mycosphaerellaceae > Septoria
Accessions
- Primary accessionO13597
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 27-47 | Helical | ||||
Sequence: IGASLISFGLPIACYAFGFLC | ||||||
Transmembrane | 100-120 | Helical | ||||
Sequence: AVLGVFFWYGLSLLLWVLLPA | ||||||
Transmembrane | 140-160 | Helical | ||||
Sequence: ACLSAVTIFVACAAGTIVRGP | ||||||
Transmembrane | 172-192 | Helical | ||||
Sequence: YIQLLTVNIIIAYALAIYVYL | ||||||
Transmembrane | 242-262 | Helical | ||||
Sequence: SFMELRPGMIGWVLLDLAFAA | ||||||
Transmembrane | 278-298 | Helical | ||||
Sequence: WTPLLLGIHVLTIHPVIVIIS | ||||||
Transmembrane | 324-344 | Helical | ||||
Sequence: FGFMLSFGDLVWVPFIYSIQA | ||||||
Transmembrane | 353-373 | Helical | ||||
Sequence: ALGPLYVALILTIQATGYYIF | ||||||
Transmembrane | 418-438 | Helical | ||||
Sequence: INYLGDWLMSWSYCLPTLAAG | ||||||
Transmembrane | 458-478 | Helical | ||||
Sequence: MKGAGIPITYFYMLYFAILLI |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000207498 | 1-512 | Delta14-sterol reductase | |||
Sequence: MARTKATARKTVPAQQEAHGYEFGGPIGASLISFGLPIACYAFGFLCNDVSGCPPPSLLSPSKLFTPPTLSNKVPWQHALDTLAAEVGWPGWSGLINTEAVLGVFFWYGLSLLLWVLLPAHEVEGTELRTGGRLKYRFNACLSAVTIFVACAAGTIVRGPDFQVWTFINRNYIQLLTVNIIIAYALAIYVYLKSFEVKAGNTEQRELAAGGHSGHILYDWYMGRELNPRITIPFIGEVDIKSFMELRPGMIGWVLLDLAFAAKQYKSYGYITDSMRKWTPLLLGIHVLTIHPVIVIISQSVYVFDALYMEPAILTTMDLTTDGFGFMLSFGDLVWVPFIYSIQAKYLSVHPVALGPLYVALILTIQATGYYIFRATNNDKNIFRTNPNDPKVAHLKYIETGTGSRLLTTGWWGTARHINYLGDWLMSWSYCLPTLAAGYKLTPTILFENSRLVSTDGMKGAGIPITYFYMLYFAILLIHRERRDEAKCRRKYGAHWEKYCQIVRWRILPGVY |
Structure
Sequence
- Sequence statusComplete
- Length512
- Mass (Da)57,578
- Last updated1998-01-01 v1
- Checksum169A756E60C0E963