O12157 · GAG_HV192
- ProteinGag polyprotein
- Genegag
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids496 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Gag polyprotein
Matrix protein p17
Matrix protein is part of the pre-integration complex. Implicated in the release from host cell mediated by Vpu. Binds to RNA (By similarity).
Capsid protein p24
The capsid promotes immune invasion by cloaking viral DNA from CGAS detection (By similarity).
Host restriction factors such as TRIM5-alpha or TRIMCyp bind retroviral capsids and cause premature capsid disassembly, leading to blocks in reverse transcription. Capsid restriction by TRIM5 is one of the factors which restricts HIV-1 to the human species. Host PIN1 apparently facilitates the virion uncoating (By similarity).
On the other hand, interactions with PDZD8 or CYPA stabilize the capsid (By similarity).
Nucleocapsid protein p7
p6-gag
Miscellaneous
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 130-131 | Cleavage; by viral protease | ||||
Sequence: YP | ||||||
Site | 361-362 | Cleavage; by viral protease | ||||
Sequence: LA | ||||||
Site | 374-375 | Cleavage; by viral protease | ||||
Sequence: MM | ||||||
Site | 429-430 | Cleavage; by viral protease | ||||
Sequence: NF | ||||||
Site | 445-446 | Cleavage; by viral protease | ||||
Sequence: LL |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Cellular Component | host cell plasma membrane | |
Cellular Component | host multivesicular body | |
Cellular Component | membrane | |
Cellular Component | viral nucleocapsid | |
Cellular Component | virion membrane | |
Molecular Function | RNA binding | |
Molecular Function | structural molecule activity | |
Molecular Function | zinc ion binding | |
Biological Process | viral budding via host ESCRT complex | |
Biological Process | viral translational frameshifting |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameGag polyprotein
- Alternative names
- Cleaved into 6 chains
Gene names
Organism names
- Taxonomic lineageViruses > Riboviria > Pararnavirae > Artverviricota > Revtraviricetes > Ortervirales > Retroviridae > Orthoretrovirinae > Lentivirus > Human immunodeficiency virus 1
- Virus hosts
Accessions
- Primary accessionO12157
Proteomes
Subcellular Location
Gag polyprotein
Matrix protein p17
Capsid protein p24
Nucleocapsid protein p7
Keywords
- Cellular component
Phenotypes & Variants
Keywords
- Disease
PTM/Processing
Features
Showing features for initiator methionine, lipidation, chain, modified residue, peptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed; by host | ||||
Sequence: M | ||||||
Lipidation | 2 | N-myristoyl glycine; by host | ||||
Sequence: G | ||||||
Chain | PRO_0000246332 | 2-130 | Matrix protein p17 | |||
Sequence: GARASILRGGKLDAWERIKLKPGGKKHYMMKHLVWASRELERFALDPGLLETSEGCKQIMKQLQPALQTGTKELISLHNTVATLYCVHEKIDVRDTKEALDKIKEEQNKSQQKTQQAEAADKGKVSQNY | ||||||
Chain | PRO_0000261202 | 2-496 | Gag polyprotein | |||
Sequence: GARASILRGGKLDAWERIKLKPGGKKHYMMKHLVWASRELERFALDPGLLETSEGCKQIMKQLQPALQTGTKELISLHNTVATLYCVHEKIDVRDTKEALDKIKEEQNKSQQKTQQAEAADKGKVSQNYPIVQNLQGQMVHQPISARTLNAWVKVVEEKAFSPEVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQMLKDTINEEAAEWDRLHPVHAGPVAPGQMREPRGSDIAGTTSTLQEQITWMTNNPPVPVGDIYKRWIILGLNKIVRMYSPVSILDIKQGPKEPFRDYVDRFFKTLRAEQATQDVKNWMTDTLLVQNANPDCKTILRALGPGASLEEMMTACQGVGGPGHKARVLAEAMSKVNNTNIMMQRSNCKGPKRTIKCFNCGKEGHLARNCRAPRKKGCWKCGKEGHQVKDCTERQANFLGKIWPSHRGRPGNLLQNRTEPTAPPEESFRFGEETTTPSRKQETIDKELPLTSLKSLFGSDPLST | ||||||
Chain | PRO_0000246333 | 131-361 | Capsid protein p24 | |||
Sequence: PIVQNLQGQMVHQPISARTLNAWVKVVEEKAFSPEVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQMLKDTINEEAAEWDRLHPVHAGPVAPGQMREPRGSDIAGTTSTLQEQITWMTNNPPVPVGDIYKRWIILGLNKIVRMYSPVSILDIKQGPKEPFRDYVDRFFKTLRAEQATQDVKNWMTDTLLVQNANPDCKTILRALGPGASLEEMMTACQGVGGPGHKARVL | ||||||
Modified residue | 146 | Phosphoserine; by host MAPK1 | ||||
Sequence: S | ||||||
Peptide | PRO_0000246334 | 362-374 | Spacer peptide 1 | |||
Sequence: AEAMSKVNNTNIM | ||||||
Chain | PRO_0000246335 | 375-429 | Nucleocapsid protein p7 | |||
Sequence: MQRSNCKGPKRTIKCFNCGKEGHLARNCRAPRKKGCWKCGKEGHQVKDCTERQAN | ||||||
Modified residue | 406 | Asymmetric dimethylarginine; in Nucleocapsid protein p7; by host PRMT6 | ||||
Sequence: R | ||||||
Peptide | PRO_0000246336 | 430-445 | Spacer peptide 2 | |||
Sequence: FLGKIWPSHRGRPGNL | ||||||
Chain | PRO_0000246337 | 446-496 | p6-gag | |||
Sequence: LQNRTEPTAPPEESFRFGEETTTPSRKQETIDKELPLTSLKSLFGSDPLST |
Post-translational modification
Matrix protein p17
Keywords
- PTM
Interaction
Subunit
Gag polyprotein
Matrix protein p17
Capsid protein p24
Interacts with host NUP98 (By similarity).
Interacts with host PPIA/CYPA; this interaction stabilizes the capsid (By similarity).
Interacts with host NUP153 (By similarity).
Interacts with host PDZD8; this interaction stabilizes the capsid. Interacts with host TRIM5; this interaction destabilizes the capsid (By similarity).
Interacts with host CPSF6 (By similarity).
Interacts with host NONO; the interaction is weak (By similarity).
Nucleocapsid protein p7
p6-gag
Family & Domains
Features
Showing features for region, motif, zinc finger, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 7-31 | Interaction with Gp41 | ||||
Sequence: ILRGGKLDAWERIKLKPGGKKHYMM | ||||||
Region | 8-43 | Interaction with host CALM1 | ||||
Sequence: LRGGKLDAWERIKLKPGGKKHYMMKHLVWASRELER | ||||||
Region | 12-19 | Interaction with host AP3D1 | ||||
Sequence: KLDAWERI | ||||||
Region | 14-33 | Interaction with membrane phosphatidylinositol 4,5-bisphosphate and RNA | ||||
Sequence: DAWERIKLKPGGKKHYMMKH | ||||||
Motif | 16-22 | Nuclear export signal | ||||
Sequence: WERIKLK | ||||||
Motif | 26-32 | Nuclear localization signal | ||||
Sequence: KKHYMMK | ||||||
Region | 73-77 | Interaction with membrane phosphatidylinositol 4,5-bisphosphate | ||||
Sequence: KELIS | ||||||
Region | 106-126 | Disordered | ||||
Sequence: EEQNKSQQKTQQAEAADKGKV | ||||||
Region | 187-225 | Interaction with host PPIA/CYPA and NUP153 | ||||
Sequence: NTVGGHQAAMQMLKDTINEEAAEWDRLHPVHAGPVAPGQ | ||||||
Region | 215-223 | PPIA/CYPA-binding loop | ||||
Sequence: PVHAGPVAP | ||||||
Region | 275-361 | Dimerization/Multimerization of capsid protein p24 | ||||
Sequence: YSPVSILDIKQGPKEPFRDYVDRFFKTLRAEQATQDVKNWMTDTLLVQNANPDCKTILRALGPGASLEEMMTACQGVGGPGHKARVL | ||||||
Zinc finger | 387-404 | CCHC-type 1 | ||||
Sequence: IKCFNCGKEGHLARNCRA | ||||||
Zinc finger | 408-425 | CCHC-type 2 | ||||
Sequence: KGCWKCGKEGHQVKDCTE | ||||||
Region | 436-482 | Disordered | ||||
Sequence: PSHRGRPGNLLQNRTEPTAPPEESFRFGEETTTPSRKQETIDKELPL | ||||||
Motif | 452-455 | PTAP/PSAP motif | ||||
Sequence: PTAP | ||||||
Compositional bias | 464-479 | Basic and acidic residues | ||||
Sequence: EETTTPSRKQETIDKE |
Domain
Sequence similarities
Keywords
- Domain
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Ribosomal frameshifting. Translation results in the formation of the Gag polyprotein most of the time. Ribosomal frameshifting at the gag-pol genes boundary occurs at low frequency and produces the Gag-Pol polyprotein. This strategy of translation probably allows the virus to modulate the quantity of each viral protein. Maintenance of a correct Gag to Gag-Pol ratio is essential for RNA dimerization and viral infectivity.
O12157-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameGag polyprotein
- NoteProduced by conventional translation.
- Length496
- Mass (Da)55,422
- Last updated2007-01-23 v3
- ChecksumB720F84AE69B266C
O12158-1
The sequence of this isoform can be found in the external entry linked below. Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
View isoform- NameGag-Pol polyprotein
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 464-479 | Basic and acidic residues | ||||
Sequence: EETTTPSRKQETIDKE |
Keywords
- Coding sequence diversity
- Technical term