O09345 · P3H2_STRSQ
- ProteinL-proline cis-3-hydroxylase 2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids290 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Dioxygenase that catalyzes the 2-oxoglutarate-dependent selective hydroxylation of free L-proline to cis-3-hydroxy-L-proline (cis-3-Hyp).
Catalytic activity
- 2-oxoglutarate + L-proline + O2 = cis-3-hydroxy-L-proline + CO2 + succinate
Cofactor
Note: Binds 1 Fe2+ ion.
Activity regulation
Inhibited by metal ions such as Co2+, Zn2+, Ni2+ or Cu2+. Is also inhibited by EDTA in vitro.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.43 mM | L-proline | |||||
0.047 mM | 2-oxoglutarate |
Comparison of both proline 3-hydroxylase isozymes shows that type II enzyme has 1.8-fold higher activity than type I upon L-proline, and 24-fold higher activity upon L-2-azetidinecarboxylic acid.
pH Dependence
Optimum pH is 6.0. Is stable from pH 5.5 to 9.0.
Temperature Dependence
Optimum temperature is 40 degrees Celsius. Is stable below 40 degrees Celsius.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | metal ion binding | |
Molecular Function | proline 3-hydroxylase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameL-proline cis-3-hydroxylase 2
- EC number
- Short namesP3H 2
- Alternative names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces
Accessions
- Primary accessionO09345
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000393427 | 1-290 | L-proline cis-3-hydroxylase 2 | |||
Sequence: MRSHILGKIELDQTRLAPDLAYLAAVPTVEEEYDEFSNGFWKHVPLWNASGDSEDRLYRDLKDAAAQPTAHVEHVPYLKEIVTTVFDGTHLQMARSRNLKNAIVIPHRDFVELDREVDRYFRTFMVLEDSPLAFHSNEDTVIHMRPGEIWFLDAATVHSAVNFSEISRQSLCVDFAFDGPFDEKEIFADATLYAPGSTPDLPERRPFTAEHRRRILSLGQVIERENFRDILFLLSKVHYKYDVHPSETYDWLIEISKQAGDEKMVVKAEQIRDFAVEARALSERFSLTSW |
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length290
- Mass (Da)33,574
- Last updated1997-07-01 v1
- ChecksumC571E1DAB598AE1A
Keywords
- Technical term