O08900 · IKZF3_MOUSE
- ProteinZinc finger protein Aiolos
- GeneIkzf3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids507 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Binds to GGGAA. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameZinc finger protein Aiolos
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionO08900
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 158 | No effect on protein abundance. Changed DNA-binding transcription factor activity. Changed sequence-specific DNA binding. Binds novel and non-specific DNA motifs and acts as a dominant negative through its homodimerization and heterodimerization activities. Profound B cell development defects observed in homozygous or heterozygous animals. Impairs the differentiation of cells in adaptive immunity. | ||||
Sequence: G → R |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 31 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000047091 | 1-507 | Zinc finger protein Aiolos | |||
Sequence: MEDIQPTVELKSTEEQPLPTESPDALNDYSLPKPHEIENVDSREAPANEDEDAGEDSMKVKDEYSDRDENIMKPEPMGDAEESEMPYSYAREYSDYESIKLERHVPYDNSRPTGGKMNCDVCGLSCISFNVLMVHKRSHTGERPFQCNQCGASFTQKGNLLRHIKLHTGEKPFKCHLCNYACQRRDALTGHLRTHSVEKPYKCEFCGRSYKQRSSLEEHKERCRAFLQNPDLGDAASVEARHIKAEMGSERALVLDRLASNVAKRKSSMPQKFIGEKRHCFDANYNPGYMYEKENEMMQTRMMDQAINNAISYLGAEALRPLVQTPPAPTSEMVPVISSVYPIALTRADMPNGAPQEMEKKRILLPEKILPSERGLSPNNSAQDSTDTDSNHEDRQHLYQQSHVVLPQARNGMPLLKEVPRSFELLKPPPICLRDSIKVINKEGEVMDVFRCDHCHVLFLDYVMFTIHMGCHGFRDPFECNMCGYRSHDRYEFSSHIARGEHRAMLK | ||||||
Modified residue | 20 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 22 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 42 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 61 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 73 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 100 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 244 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 325 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 377 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expression is restricted to lymphoid tissues. Expressed at highest levels in spleen and at lower levels in the thymus and bone marrow. First detected in more committed lymphoid progenitors and strongly up-regulated as these differentiate into pre-T and pre-B cell precursors.
Gene expression databases
Interaction
Subunit
Homodimer. Heterodimer with other IKAROS family members. Interacts with IKZF4 and IKZF5. Interacts with HRAS. Interacts with FOXP3; this interaction may be required for silencing target genes and regulating the suppressive activity of FOXP3-positive regulatory T-cells (Treg). Interacts with BCL21L isoform Bcl-X(L); this interaction blocks the anti-apoptotic role of BCL21L. Associates with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A (By similarity).
Interacts with IKZF1
Interacts with IKZF1
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-85 | Disordered | ||||
Sequence: MEDIQPTVELKSTEEQPLPTESPDALNDYSLPKPHEIENVDSREAPANEDEDAGEDSMKVKDEYSDRDENIMKPEPMGDAEESEM | ||||||
Compositional bias | 34-85 | Basic and acidic residues | ||||
Sequence: PHEIENVDSREAPANEDEDAGEDSMKVKDEYSDRDENIMKPEPMGDAEESEM | ||||||
Zinc finger | 117-139 | C2H2-type 1 | ||||
Sequence: MNCDVCGLSCISFNVLMVHKRSH | ||||||
Zinc finger | 145-167 | C2H2-type 2 | ||||
Sequence: FQCNQCGASFTQKGNLLRHIKLH | ||||||
Zinc finger | 173-195 | C2H2-type 3 | ||||
Sequence: FKCHLCNYACQRRDALTGHLRTH | ||||||
Zinc finger | 201-223 | C2H2-type 4; atypical | ||||
Sequence: YKCEFCGRSYKQRSSLEEHKERC | ||||||
Region | 370-396 | Disordered | ||||
Sequence: LPSERGLSPNNSAQDSTDTDSNHEDRQ | ||||||
Zinc finger | 450-472 | C2H2-type 5 | ||||
Sequence: FRCDHCHVLFLDYVMFTIHMGCH | ||||||
Region | 450-502 | Mediates homodimerization and heterodimerization | ||||
Sequence: FRCDHCHVLFLDYVMFTIHMGCHGFRDPFECNMCGYRSHDRYEFSSHIARGEH | ||||||
Zinc finger | 478-502 | C2H2-type 6; atypical | ||||
Sequence: FECNMCGYRSHDRYEFSSHIARGEH |
Domain
C2H2-type 5 and C2H2-type 6 mediate homodimerization and heterodimerization.
Sequence similarities
Belongs to the Ikaros C2H2-type zinc-finger protein family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length507
- Mass (Da)57,967
- Last updated2011-07-27 v2
- Checksum103D46D0C6E236F7
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 34-85 | Basic and acidic residues | ||||
Sequence: PHEIENVDSREAPANEDEDAGEDSMKVKDEYSDRDENIMKPEPMGDAEESEM | ||||||
Sequence conflict | 114 | in Ref. 1; AAB58795 | ||||
Sequence: G → S | ||||||
Sequence conflict | 319 | in Ref. 1; AAB58795 | ||||
Sequence: L → F | ||||||
Sequence conflict | 352 | in Ref. 1; AAB58795 | ||||
Sequence: N → M |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF001293 EMBL· GenBank· DDBJ | AAB58795.1 EMBL· GenBank· DDBJ | mRNA | ||
AL591125 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL591390 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH466556 EMBL· GenBank· DDBJ | EDL16146.1 EMBL· GenBank· DDBJ | Genomic DNA |