O08721 · UNC5A_RAT

  • Protein
    Netrin receptor UNC5A
  • Gene
    Unc5a
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Receptor for netrin required for axon guidance (PubMed:10399920, PubMed:9126742).
Functions in the netrin signaling pathway and promotes neurite outgrowth in response to NTN1 (PubMed:19755150).
Mediates axon repulsion of neuronal growth cones in the developing nervous system in response to netrin (PubMed:10399920).
Axon repulsion in growth cones may be mediated by its association with DCC that may trigger signaling for repulsion (PubMed:10399920).
It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed:11387206, PubMed:12598531).

Features

Showing features for site.

1898100200300400500600700800
TypeIDPosition(s)Description
Site396-397Cleavage; by caspase-3

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentmembrane raft
Cellular Componentneuron projection membrane
Cellular Componentneuronal cell body membrane
Cellular Componentplasma membrane
Molecular Functionnetrin receptor activity
Biological Processanterior/posterior axon guidance
Biological Processapoptotic process
Biological Processaxon guidance
Biological Processnetrin-activated signaling pathway
Biological Processneuron projection development

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Netrin receptor UNC5A
  • Alternative names
    • Protein unc-5 homolog 1
    • Protein unc-5 homolog A

Gene names

    • Name
      Unc5a
    • Synonyms
      Unc5h1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    O08721

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Single-pass type I membrane protein
Membrane raft
Note: The interaction with PRKCABP regulates its surface expression and leads to its removal from the surface of neurons and growth cones (PubMed:14672991).

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain26-361Extracellular
Transmembrane362-382Helical
Topological domain383-898Cytoplasmic

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis896-898Abolishes interaction with PRKCABP.

PTM/Processing

Features

Showing features for signal, chain, disulfide bond, glycosylation.

TypeIDPosition(s)Description
Signal1-25
ChainPRO_000003607026-898Netrin receptor UNC5A
Disulfide bond65↔126
Disulfide bond77↔124
Glycosylation107N-linked (GlcNAc...) asparagine
Disulfide bond170↔221
Glycosylation218N-linked (GlcNAc...) asparagine
Glycosylation245C-linked (Man) tryptophan
Glycosylation248C-linked (Man) tryptophan
Glycosylation251C-linked (Man) tryptophan
Disulfide bond254↔291
Disulfide bond258↔295
Disulfide bond269↔281
Glycosylation301C-linked (Man) tryptophan
Glycosylation304C-linked (Man) tryptophan
Disulfide bond310↔344
Disulfide bond314↔349
Disulfide bond322↔334
Glycosylation343N-linked (GlcNAc...) asparagine

Post-translational modification

Phosphorylated on cytoplasmic tyrosine residues (By similarity).
Phosphorylated by PKC in vitro
Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
The two extracellular TSRs of UNC5A contain WxxWxxWxxC motifs that can be C-mannosylated on all tryptophans. DPY19L1 preferentially mannosylates the first two tryptophans and DPY19L3 prefers the third. C-mannosylation by DPY19L1 is required for transport of UNC5A from the endoplasmic reticulum to the cell surface.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Mainly expressed in regions of differentiating neurons. Expressed at early stages of neural tube development in the ventral spinal cord. In developing hindbrain, it colocalizes with a number of cranial motor neuron subpopulations from embryonic E11 to E14, while DCC is expressed by motor neurons at E12. Also expressed in non-neural structures, such as the basal plane of the hindbrain and midbrain, in the developing hypothalamus, thalamus and in the pallidum.

Interaction

Subunit

Homodimer and homooligomer (PubMed:19755150).
Interacts with the cytoplasmic part of DCC (PubMed:10399920).
Interacts with MAGED1 (PubMed:12598531).
Interacts with PRKCABP, possibly mediating some interaction with PKC (PubMed:14672991).
Interacts (via extracellular domain) with FLRT2 (via extracellular domain) (By similarity).
Interacts (via extracellular domain) with FLRT3 (via extracellular domain) (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain44-141Ig-like
Domain155-238Ig-like C2-type
Domain242-296TSP type-1 1
Domain298-350TSP type-1 2
Domain497-640ZU5
Region661-679Interaction with DCC
Domain817-897Death

Domain

The ZU5 domain mediates the interaction with MAGED1, which participates in the induction of apoptosis.

Sequence similarities

Belongs to the unc-5 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    898
  • Mass (Da)
    98,841
  • Last updated
    1997-07-01 v1
  • Checksum
    7A3CBCB9E7ACA135
MAVRPGLWPVLLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDSSSGLPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIAYLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSTSAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVQKTACATLCPVDGSWSSWSKWSACGLDCTHWRSRECSDPAPRNGGEECRGADLDTRNCTSDLCLHTASCPEDVALYIGLVAVAVCLFLLLLALGLIYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTTYQGSLCSRQDGPSPKFQLSNGHLLSPLGSGRHTLHHSSPTSEAEDFVSRLSTQNYFRSLPRGTSNMAYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPVVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEDVLHLGEESPSHLYYCQLEAGACYVFTEQLGRFALVGEALSVAATKRLRLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQQYLHCTFTLERINASTSDLACKVWVWQVEGDGQSFNINFNITKDTRFAELLALESEGGVPALVGPSAFKIPFLIRQKIIASLDPPCSRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLGQLAAAVAGLGQPDAGLFTVSEAEC

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0G2JZN2A0A0G2JZN2_RATUnc5a842
A0A8I5YBB0A0A8I5YBB0_RATUnc5a898

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U87305
EMBL· GenBank· DDBJ
AAB57678.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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