O08705 · NTCP_MOUSE
- ProteinHepatic sodium/bile acid cotransporter
- GeneSlc10a1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids362 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
As a major transporter of conjugated bile salts from plasma into the hepatocyte, it plays a key role in the enterohepatic circulation of bile salts necessary for the solubilization and absorption of dietary fat and fat-soluble vitamins (PubMed:10209268).
It is strictly dependent on the extracellular presence of sodium (PubMed:10209268, PubMed:34060352).
It exhibits broad substrate specificity and transports various bile acids, such as taurocholate, cholate, as well as non-bile acid organic compounds, such as estrone sulfate (PubMed:10209268, PubMed:34060352).
Works collaboratively with the ileal transporter (NTCP2), the organic solute transporter (OST), and the bile salt export pump (BSEP), to ensure efficacious biological recycling of bile acids during enterohepatic circulation (By similarity).
It is strictly dependent on the extracellular presence of sodium (PubMed:10209268, PubMed:34060352).
It exhibits broad substrate specificity and transports various bile acids, such as taurocholate, cholate, as well as non-bile acid organic compounds, such as estrone sulfate (PubMed:10209268, PubMed:34060352).
Works collaboratively with the ileal transporter (NTCP2), the organic solute transporter (OST), and the bile salt export pump (BSEP), to ensure efficacious biological recycling of bile acids during enterohepatic circulation (By similarity).
Catalytic activity
Isoform 1
2 Na+(out) + taurocholate(out) = 2 Na+(in) + taurocholate(in)2 Na+ (out)CHEBI:29101+ taurocholate (out)CHEBI:36257= 2 Na+ (in)CHEBI:29101+ taurocholate (in)CHEBI:36257Isoform 2
2 Na+(out) + taurocholate(out) = 2 Na+(in) + taurocholate(in)2 Na+ (out)CHEBI:29101+ taurocholate (out)CHEBI:36257= 2 Na+ (in)CHEBI:29101+ taurocholate (in)CHEBI:36257- estrone 3-sulfate(out) + 2 Na+(out) = estrone 3-sulfate(in) + 2 Na+(in)
- cholate(out) + 2 Na+(out) = cholate(in) + 2 Na+(in)
- 2 Na+(out) + taurochenodeoxycholate(out) = 2 Na+(in) + taurochenodeoxycholate(in)
- 2 Na+(out) + tauroursodeoxycholate(out) = 2 Na+(in) + tauroursodeoxycholate(in)
- glycocholate(out) + 2 Na+(out) = glycocholate(in) + 2 Na+(in)
- 2 Na+(out) + tauronorcholate(out) = 2 Na+(in) + tauronorcholate(in)
- 2 Na+(out) + taurodeoxycholate(out) = 2 Na+(in) + taurodeoxycholate(in)
- 2 Na+(out) + tauroallocholate(out) = 2 Na+(in) + tauroallocholate(in)
- 2 Na+(out) + taurohyodeoxycholate(out) = 2 Na+(in) + taurohyodeoxycholate(in)
- 2 Na+(out) + taurohyocholate(out) = 2 Na+(in) + taurohyocholate(in)
- 2 Na+(out) + tauro-beta-muricholate(out) = 2 Na+(in) + tauro-beta-muricholate(in)
Kinetics
Isoform 1
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
86 μM | taurocholate | |||||
104 μM | estrone 3-sulfate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
3004 pmol/min/mg | with estrone 3-sulfate as substrate |
Isoform 2
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
14 μM | taurocholate |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | basolateral plasma membrane | |
Cellular Component | membrane | |
Molecular Function | bile acid transmembrane transporter activity | |
Molecular Function | bile acid:sodium symporter activity | |
Biological Process | bile acid and bile salt transport | |
Biological Process | bile acid signaling pathway | |
Biological Process | cellular response to xenobiotic stimulus | |
Biological Process | regulation of bile acid secretion | |
Biological Process | response to estrogen | |
Biological Process | response to ethanol | |
Biological Process | response to nutrient levels | |
Biological Process | response to organic cyclic compound |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHepatic sodium/bile acid cotransporter
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionO08705
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 24-45 | Helical | ||||
Sequence: DTALSVILVVMLLLIMLSLGCT | ||||||
Transmembrane | 60-80 | Helical | ||||
Sequence: GVIIAIVAQYGIMPLSAFLLG | ||||||
Transmembrane | 82-98 | Helical | ||||
Sequence: VFHLTSIEALAILICGC | ||||||
Transmembrane | 158-178 | Helical | ||||
Sequence: GIMLSLVMVLIPCAIGIFLKS | ||||||
Transmembrane | 190-211 | Helical | ||||
Sequence: AGMIITFSLSVAVTVLSVINVG | ||||||
Transmembrane | 228-244 | Helical | ||||
Sequence: LMPFTGFLMGYILSALF | ||||||
Transmembrane | 285-306 | Helical | ||||
Sequence: FPLLYMIFQLAEGLLFIIIFRC |
Keywords
- Cellular component
Phenotypes & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 23 variants from UniProt as well as other sources including ClinVar and dbSNP.
Chemistry
PTM/Processing
Features
Showing features for chain, glycosylation, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000052336 | 1-362 | Hepatic sodium/bile acid cotransporter | |||
Sequence: MEAHNVSAPFNFSLPPGFGHRATDTALSVILVVMLLLIMLSLGCTMEFSKIKAHFWKPKGVIIAIVAQYGIMPLSAFLLGKVFHLTSIEALAILICGCSPGGNLSNLFTLAMKGDMNLSIVMTTCSSFTALGMMPLLLYIYSKGIYDGDLKDKVPYKGIMLSLVMVLIPCAIGIFLKSKRPHYVPYVLKAGMIITFSLSVAVTVLSVINVGNSIMFVMTPHLLATSSLMPFTGFLMGYILSALFRLNPSCRRTISMETGFQNVQLCSTILNVTFPPEVIGPLFFFPLLYMIFQLAEGLLFIIIFRCYLKIKPQKDQTKITYKAAATEDATPAALEKGTHNGNNPPTQPGLSPNGLNSGQMAN | ||||||
Glycosylation | 5 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 11 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 103 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 117 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 271 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 330 | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 332-362 | Disordered | ||||
Sequence: AALEKGTHNGNNPPTQPGLSPNGLNSGQMAN | ||||||
Compositional bias | 336-362 | Polar residues | ||||
Sequence: KGTHNGNNPPTQPGLSPNGLNSGQMAN |
Sequence similarities
Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
O08705-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length362
- Mass (Da)39,413
- Last updated1997-07-01 v1
- Checksum7A70493E1804280F
O08705-2
- Name2
- Differences from canonical
- 318-362: Missing
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1W2P719 | A0A1W2P719_MOUSE | Slc10a1 | 66 | ||
A0A8V5KH54 | A0A8V5KH54_MOUSE | Slc10a1 | 317 |
Features
Showing features for sequence conflict, alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 315-317 | in Ref. 4 and 5; AAB81024 | ||||
Sequence: DQT → GKY | ||||||
Alternative sequence | VSP_061644 | 318-362 | in isoform 2 | |||
Sequence: Missing | ||||||
Compositional bias | 336-362 | Polar residues | ||||
Sequence: KGTHNGNNPPTQPGLSPNGLNSGQMAN |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB003303 EMBL· GenBank· DDBJ | BAA19846.1 EMBL· GenBank· DDBJ | mRNA | ||
U95131 EMBL· GenBank· DDBJ | AAB81023.1 EMBL· GenBank· DDBJ | mRNA | ||
BC021154 EMBL· GenBank· DDBJ | AAH21154.1 EMBL· GenBank· DDBJ | mRNA | ||
BC094023 EMBL· GenBank· DDBJ | AAH94023.1 EMBL· GenBank· DDBJ | mRNA | ||
U95132 EMBL· GenBank· DDBJ | AAB81024.1 EMBL· GenBank· DDBJ | mRNA |