O08705 · NTCP_MOUSE

  • Protein
    Hepatic sodium/bile acid cotransporter
  • Gene
    Slc10a1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

As a major transporter of conjugated bile salts from plasma into the hepatocyte, it plays a key role in the enterohepatic circulation of bile salts necessary for the solubilization and absorption of dietary fat and fat-soluble vitamins (PubMed:10209268).
It is strictly dependent on the extracellular presence of sodium (PubMed:10209268, PubMed:34060352).
It exhibits broad substrate specificity and transports various bile acids, such as taurocholate, cholate, as well as non-bile acid organic compounds, such as estrone sulfate (PubMed:10209268, PubMed:34060352).
Works collaboratively with the ileal transporter (NTCP2), the organic solute transporter (OST), and the bile salt export pump (BSEP), to ensure efficacious biological recycling of bile acids during enterohepatic circulation (By similarity).

Catalytic activity

  • Isoform 1

    2 Na+(out) + taurocholate(out) = 2 Na+(in) + taurocholate(in)
  • Isoform 2

    2 Na+(out) + taurocholate(out) = 2 Na+(in) + taurocholate(in)
  • estrone 3-sulfate(out) + 2 Na+(out) = estrone 3-sulfate(in) + 2 Na+(in)
  • cholate(out) + 2 Na+(out) = cholate(in) + 2 Na+(in)
  • 2 Na+(out) + taurochenodeoxycholate(out) = 2 Na+(in) + taurochenodeoxycholate(in)
  • 2 Na+(out) + tauroursodeoxycholate(out) = 2 Na+(in) + tauroursodeoxycholate(in)
  • glycocholate(out) + 2 Na+(out) = glycocholate(in) + 2 Na+(in)
  • 2 Na+(out) + tauronorcholate(out) = 2 Na+(in) + tauronorcholate(in)
  • 2 Na+(out) + taurodeoxycholate(out) = 2 Na+(in) + taurodeoxycholate(in)
  • 2 Na+(out) + tauroallocholate(out) = 2 Na+(in) + tauroallocholate(in)
  • 2 Na+(out) + taurohyodeoxycholate(out) = 2 Na+(in) + taurohyodeoxycholate(in)
  • 2 Na+(out) + taurohyocholate(out) = 2 Na+(in) + taurohyocholate(in)
  • 2 Na+(out) + tauro-beta-muricholate(out) = 2 Na+(in) + tauro-beta-muricholate(in)

Kinetics

Isoform 1

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
86 μMtaurocholate
104 μMestrone 3-sulfate
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
3004 pmol/min/mgwith estrone 3-sulfate as substrate

Isoform 2

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
14 μMtaurocholate

GO annotations

AspectTerm
Cellular Componentbasolateral plasma membrane
Cellular Componentmembrane
Molecular Functionbile acid transmembrane transporter activity
Molecular Functionbile acid:sodium symporter activity
Biological Processbile acid and bile salt transport
Biological Processbile acid signaling pathway
Biological Processcellular response to xenobiotic stimulus
Biological Processregulation of bile acid secretion
Biological Processresponse to estrogen
Biological Processresponse to ethanol
Biological Processresponse to nutrient levels
Biological Processresponse to organic cyclic compound

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Hepatic sodium/bile acid cotransporter
  • Alternative names
    • Na(+)/bile acid cotransporter
    • Na(+)/taurocholate transport protein
    • Sodium/taurocholate cotransporting polypeptide (NTCP)
    • Solute carrier family 10 member 1 (SLC10A1)

Gene names

    • Name
      Slc10a1
    • Synonyms
      Ntcp

Organism names

  • Taxonomic identifier
  • Strains
    • BALB/cJ
    • FVB/N
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    O08705
  • Secondary accessions
    • O35940
    • Q52L86

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane24-45Helical
Transmembrane60-80Helical
Transmembrane82-98Helical
Transmembrane158-178Helical
Transmembrane190-211Helical
Transmembrane228-244Helical
Transmembrane285-306Helical

Keywords

Phenotypes & Variants

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 23 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Chemistry

PTM/Processing

Features

Showing features for chain, glycosylation, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000523361-362Hepatic sodium/bile acid cotransporter
Glycosylation5N-linked (GlcNAc...) asparagine
Glycosylation11N-linked (GlcNAc...) asparagine
Glycosylation103N-linked (GlcNAc...) asparagine
Glycosylation117N-linked (GlcNAc...) asparagine
Glycosylation271N-linked (GlcNAc...) asparagine
Modified residue330Phosphothreonine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Isoform 1

Expressed in liver in higher amounts than isoform 2.

Isoform 2

Expressed in liver in lower amounts than isoform 1.

Gene expression databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region332-362Disordered
Compositional bias336-362Polar residues

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

O08705-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    362
  • Mass (Da)
    39,413
  • Last updated
    1997-07-01 v1
  • Checksum
    7A70493E1804280F
MEAHNVSAPFNFSLPPGFGHRATDTALSVILVVMLLLIMLSLGCTMEFSKIKAHFWKPKGVIIAIVAQYGIMPLSAFLLGKVFHLTSIEALAILICGCSPGGNLSNLFTLAMKGDMNLSIVMTTCSSFTALGMMPLLLYIYSKGIYDGDLKDKVPYKGIMLSLVMVLIPCAIGIFLKSKRPHYVPYVLKAGMIITFSLSVAVTVLSVINVGNSIMFVMTPHLLATSSLMPFTGFLMGYILSALFRLNPSCRRTISMETGFQNVQLCSTILNVTFPPEVIGPLFFFPLLYMIFQLAEGLLFIIIFRCYLKIKPQKDQTKITYKAAATEDATPAALEKGTHNGNNPPTQPGLSPNGLNSGQMAN

O08705-2

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1W2P719A0A1W2P719_MOUSESlc10a166
A0A8V5KH54A0A8V5KH54_MOUSESlc10a1317

Features

Showing features for sequence conflict, alternative sequence, compositional bias.

TypeIDPosition(s)Description
Sequence conflict315-317in Ref. 4 and 5; AAB81024
Alternative sequenceVSP_061644318-362in isoform 2
Compositional bias336-362Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB003303
EMBL· GenBank· DDBJ
BAA19846.1
EMBL· GenBank· DDBJ
mRNA
U95131
EMBL· GenBank· DDBJ
AAB81023.1
EMBL· GenBank· DDBJ
mRNA
BC021154
EMBL· GenBank· DDBJ
AAH21154.1
EMBL· GenBank· DDBJ
mRNA
BC094023
EMBL· GenBank· DDBJ
AAH94023.1
EMBL· GenBank· DDBJ
mRNA
U95132
EMBL· GenBank· DDBJ
AAB81024.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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