O08689 · GDF8_MOUSE

  • Protein
    Growth/differentiation factor 8
  • Gene
    Mstn
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Acts specifically as a negative regulator of skeletal muscle growth.

Features

Showing features for site.

137650100150200250300350
TypeIDPosition(s)Description
Site99-100Cleavage

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Cellular Componentextracellular space
Molecular Functioncytokine activity
Molecular Functiongrowth factor activity
Molecular Functionheparin binding
Molecular Functionidentical protein binding
Molecular Functionprotein homodimerization activity
Molecular Functionsignaling receptor binding
Biological Processcellular response to dexamethasone stimulus
Biological Processcellular response to hypoxia
Biological Processmuscle cell cellular homeostasis
Biological Processmyoblast migration involved in skeletal muscle regeneration
Biological Processnegative regulation of insulin receptor signaling pathway
Biological Processnegative regulation of kinase activity
Biological Processnegative regulation of muscle hypertrophy
Biological Processnegative regulation of myoblast differentiation
Biological Processnegative regulation of myoblast proliferation
Biological Processnegative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
Biological Processnegative regulation of satellite cell differentiation
Biological Processnegative regulation of skeletal muscle satellite cell proliferation
Biological Processnegative regulation of skeletal muscle tissue growth
Biological Processovulation cycle process
Biological Processpositive regulation of DNA-templated transcription
Biological Processpositive regulation of lamellipodium assembly
Biological Processpositive regulation of macrophage chemotaxis
Biological Processresponse to electrical stimulus
Biological Processresponse to estrogen
Biological Processresponse to ethanol
Biological Processresponse to gravity
Biological Processresponse to muscle activity
Biological Processresponse to testosterone
Biological Processskeletal muscle atrophy
Biological Processskeletal muscle satellite cell differentiation
Biological Processskeletal muscle tissue regeneration
Biological Processtransforming growth factor beta receptor signaling pathway

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Growth/differentiation factor 8
  • Short names
    GDF-8
  • Alternative names
    • Myostatin

Gene names

    • Name
      Mstn
    • Synonyms
      Gdf8

Organism names

  • Taxonomic identifier
  • Strain
    • CD-1
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    O08689

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Mutant animals exhibit muscle hypertrophy.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis99No effect on proteolytic cleavage.
Mutagenesis100Blocks proteolytic cleavage; increases muscle mass when injected into adult mice.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Chemistry

PTM/Processing

Features

Showing features for signal, propeptide, glycosylation, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-24
PropeptidePRO_000003395625-267
Glycosylation72N-linked (GlcNAc...) asparagine
ChainPRO_0000033957268-376Growth/differentiation factor 8
Disulfide bond273↔283
Disulfide bond282↔341
Disulfide bond310↔373
Disulfide bond314↔375
Disulfide bond340Interchain

Post-translational modification

Synthesized as large precursor molecule that undergoes proteolytic cleavage to generate an N-terminal propeptide and a disulfide linked C-terminal dimer, which is the biologically active molecule. The circulating form consists of a latent complex of the C-terminal dimer and other proteins, including its propeptide, which maintain the C-terminal dimer in a latent, inactive state. Ligand activation requires additional cleavage of the prodomain by a tolloid-like metalloproteinase (PubMed:14671324).

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue.

Developmental stage

First detected 9.5 dpc in one-third of developing somites. At 10.5 dpc, expressed in the myotome compartment of somites. At later stages of development, detected in a wide range of developing muscles. Expression continues in adulthood.

Gene expression databases

Interaction

Subunit

Homodimer; disulfide-linked (PubMed:19644449).
Interacts with WFIKKN2, leading to inhibit its activity (PubMed:12595574).
Interacts with FSTL3 (PubMed:22052913).

Protein-protein interaction databases

Chemistry

Miscellaneous

Family & Domains

Sequence similarities

Belongs to the TGF-beta family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    376
  • Mass (Da)
    42,921
  • Last updated
    1997-07-01 v1
  • Checksum
    3E19814DD62C08BE
MMQKLQMYVYIYLFMLIAAGPVDLNEGSEREENVEKEGLCNACAWRQNTRYSRIEAIKIQILSKLRLETAPNISKDAIRQLLPRAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQADGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVKTPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMSPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPAMVVDRCGCS

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A087WQL8A0A087WQL8_MOUSEMstn189

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U84005
EMBL· GenBank· DDBJ
AAC53167.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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