O08574 · MESP2_MOUSE

  • Protein
    Mesoderm posterior protein 2
  • Gene
    Mesp2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Transcription factor with important role in somitogenesis. Defines the rostrocaudal patterning of the somite by participating in distinct Notch pathways. Regulates also the FGF signaling pathway. Specifies the rostral half of the somites. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand. Participates in the segment border formation by activating in the anterior presomitic mesoderm LFNG, a negative regulator of DLL1-Notch signaling. Acts as a strong suppressor of Notch activity. Together with MESP1 is involved in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. May play a role with Tcf15 in the differentiation of myotomal and sclerotomal cells by regulating Pax family genes. Controls also the expression of the protocadherin PCDH8/PAPC, EPHA4, RIPPLY2, NOTCH2, FGFR1, and CER1. Binds to the E-boxes within the EPH4A and RIPPLY2 enhancers.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnucleoplasm
Cellular Componentnucleus
Molecular FunctionDNA-binding transcription activator activity, RNA polymerase II-specific
Molecular FunctionDNA-binding transcription factor activity
Molecular FunctionDNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Functionprotein dimerization activity
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Biological Processheart morphogenesis
Biological Processmesoderm formation
Biological Processmesodermal cell migration
Biological ProcessNotch signaling pathway
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processregulation of transcription by RNA polymerase II
Biological Processsignal transduction involved in regulation of gene expression
Biological Processsomite rostral/caudal axis specification
Biological Processsomitogenesis

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Mesoderm posterior protein 2

Gene names

    • Name
      Mesp2

Organism names

  • Taxonomic identifier
  • Strains
    • ICR
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    O08574
  • Secondary accessions
    • Q3UIV5

Proteomes

Organism-specific databases

Subcellular Location

Nucleus

Keywords

Phenotypes & Variants

Disruption phenotype

Mice die shortly after birth and show caudal truncation and severe skeletal malformations. Lack of segmentation and impaired segment polarity of the paraxial mesoderm are the primary defects. Mutants show altered expression of MEOX1, Pax1, and DLL1 and lack of expression of Notch1, Notch2, and FGFR1. Mice lacking Mesp1 and Mesp2 die around 9.5 dpc. The major defect is the apparent lack of any mesodermal layer between endoderm and ectoderm and a defect in the migratory activity of mesodermal cells.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis70-73Localizes in nucleus and cytoplasm. Localizes mainly in cytoplasm; when associated with 145-A--A-150.
Mutagenesis145-150Localizes in nucleus and cytoplasm. Localizes mainly in cytoplasm; when associated with 70-A--A-73.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002963021-370Mesoderm posterior protein 2

Post-translational modification

Degraded by the proteasome.
Phosphorylated.

Keywords

PTM databases

Expression

Induction

Down-regulated in NOTCH1, DLL1, RBPJ and FOXC1/FOXC2 mutant mice. Expression in the anterior presomitic mesoderm is periodic, dissipating once the segmental border is established. Negatively regulated by RIPPLY2. Positively regulated by TBX6 and Notch signaling.

Developmental stage

First transiently detected in the nascent mesoderm at the onset of gastrulation. A second site of expression is detected at 8 dpc in the rostral region of the presomitic mesoderm inmediately before segmentation. Down-regulated immediately after the formation of the segmented somites before 13.5 dpc. Initially expressed throughout the length of one somite, and then quickly repressed in the presumptive caudal region. Mesp2 is involved in the rapid down-regulation of its own expression in the presumptive caudal half of the somite.

Interaction

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region51-89Disordered
Compositional bias54-70Polar residues
Domain79-133bHLH
Region231-265Disordered
Region325-350Disordered
Region326-330May contain a degradation domain

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    370
  • Mass (Da)
    39,789
  • Last updated
    1997-07-01 v1
  • Checksum
    31CAB72E2398150D
MAQSSPPQSLQGLVPLGLLPGLGLGSAIGLHVSGLVLRFVRFLPFYATRRPSQPAGPARSTRTTQATAPRRTRPAPAGGQRQSASEREKLRMRTLARALQELRRFLPPSVAPAGQSLTKIETLRLAIRYIGHLSALLGLSEDSLRRRRRRSADAAFSHRCPQCPDGGSPSQAQMLGPSLGSAMSSGVSWGCPPACPGPLISPENLGNRISNVDPRVTPPYCPQIQSPLHQSLERAADSSPWAPPQACPGMQMSPEPRNKTGHWTQSTEPAELTKVYQSLSVSPEPRLSLGSPLLLPRPSCQRLQPQPQPQPQWGCWGHDAEVLSTSEDQGSSPALQLPVASPTPSSGLQLSGCPELWQEDLEGPPLNIFY

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A6H5V4A6H5V4_MOUSEMesp2370

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias54-70Polar residues
Sequence conflict107in Ref. 2; BAE27401
Sequence conflict215in Ref. 2; BAE27401
Sequence conflict286in Ref. 2; BAE27401

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U71125
EMBL· GenBank· DDBJ
AAB51199.1
EMBL· GenBank· DDBJ
mRNA
AK146740
EMBL· GenBank· DDBJ
BAE27401.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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