O07576 · BCAP_BACSU

Function

function

Branched-chain amino acid transport system which is involved in the uptake of isoleucine, valine and probably leucine (PubMed:25645558).
Can also transport threonine, and is active as a minor serine permease (PubMed:25645558, PubMed:32743959).
May be an amino acid permease of rather broad specificity, because several amino acids, albeit at 100-fold excess, are able to prevent isoleucine uptake (PubMed:25645558).
Probably does not transport methionine (PubMed:25645558).
Together with BraB and BrnQ, plays an important role in the activation of CodY, a branched-chain amino acid-responsive transcriptional regulator that controls the expression of several dozen transcription units in B.subtilis (PubMed:25645558).

Activity regulation

Isoleucine uptake is efficiently reduced in the presence of 100-fold excess valine, leucine, alanine, threonine, serine, cysteine, asparagine, and a nonproteinaceous amino acid 4-azaleucine.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
4.1 μMisoleucine
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
15 nmol/min/mgwith isoleucine as substrate

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Functionamino acid transmembrane transporter activity
Biological Processamino acid transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Branched-chain amino acid permease BcaP
  • Short names
    BCAA permease

Gene names

    • Name
      bcaP
    • Synonyms
      yhdG
    • Ordered locus names
      BSU09460

Organism names

Accessions

  • Primary accession
    O07576
  • Secondary accessions
    • Q796X7

Proteomes

Subcellular Location

Cell membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane35-55Helical
Transmembrane57-77Helical
Transmembrane103-123Helical
Transmembrane133-153Helical
Transmembrane159-179Helical
Transmembrane188-208Helical
Transmembrane216-236Helical
Transmembrane258-278Helical
Transmembrane305-325Helical
Transmembrane355-375Helical
Transmembrane380-400Helical
Transmembrane413-432Helical
Transmembrane437-456Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Mutant is still able to take up isoleucine, leucine and valine (PubMed:25645558).
Deletion of the gene confers a weak resistance to growth inhibition by serine (PubMed:32743959).
The deletion of the three permease-encoding genes aimA (ybeC), ybxG and bcaP results in an unprecedented resistance to serine up to 100 mM (PubMed:32743959).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003608361-465Branched-chain amino acid permease BcaP

Proteomic databases

Expression

Induction

Expression is repressed by the transcriptional regulator CodY (PubMed:12618455, PubMed:21097623).
Contains two CodY-binding sites in the bcaP regulatory region, which both contribute to repression in vivo and do so independently of each other (PubMed:21097623).
A single CodY-binding site is apparently sufficient for substantial CodY-dependent regulation, but both upstream and downstram CodY-binding regions are required for maximal repression of bcaP (PubMed:21097623).
Efficient bcaP repression by CodY requires the simultaneous presence of isoleucine, leucine and valine, and other amino acids in the growth medium (PubMed:21097623).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    465
  • Mass (Da)
    49,711
  • Last updated
    1997-07-01 v1
  • Checksum
    CC4A815DBA426A94
MKGSVFRKKSIQDLIAATSGEKSLKRELGAFDLTLLGIGAIIGTGIFVLTGTGAVTAGPGLTISFVVAALACLFAALSYAEFASSVPVSGSVYTFTYATLGELMAFIIGWDLILEYMLAVSAVSVGWSGYFQSFLSGLGIHLPVALTAAPGAVKGTFTLFNLPAFVIVMAITYLLYLGIKESKRVNNIMVILKILVVLLFIAVAAVYVKPHNWQPFMPMGFGGVFSAAALVFFAFIGFDAVSSAAEETKNPAKDLPKGIIFSLLVCTILYVTVSAIMTGVIPFAQFAGVDHPVSLVLQSAGQNWVAGIIDIGAVLGMTTVMLVMLYGQTRVMFAMSRDGLVPGSLSKVHPKHKTPYVATWFFGTMSALLGSLVPLDELAKLVNIGTLSAFVLISVAVIVLRKKQPDLPRAFKCPGVPVIPGLAILFCLFLILNLGWVTIVRFLVWLLIGLVIYFLYSRKHSKLNQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Y14082
EMBL· GenBank· DDBJ
CAA74491.1
EMBL· GenBank· DDBJ
Genomic DNA
AL009126
EMBL· GenBank· DDBJ
CAB12785.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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