O06988 · BBMA_BACSU
- ProteinIntracellular maltogenic amylase
- GenebbmA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids588 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferring glucose or maltose to another moiety of sugars by forming alpha-(1,6)- and alpha-(1,3)-glycosidic linkages upon the hydrolysis of substrate at concentrations of 5% or higher (By similarity).
Cofactor
Note: Binds 1 Ca2+ ion per subunit.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 149 | Ca2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 155 | Ca2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 174 | Ca2+ (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 176 | Ca2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 249 | substrate | ||||
Sequence: H | ||||||
Binding site | 325 | substrate | ||||
Sequence: R | ||||||
Active site | 327 | Nucleophile | ||||
Sequence: D | ||||||
Active site | 356 | Proton donor | ||||
Sequence: E | ||||||
Binding site | 422-423 | substrate | ||||
Sequence: HD | ||||||
Site | 423 | Transition state stabilizer | ||||
Sequence: D | ||||||
Binding site | 467 | substrate | ||||
Sequence: D | ||||||
Binding site | 471 | substrate | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | hydrolase activity, hydrolyzing O-glycosyl compounds | |
Molecular Function | metal ion binding | |
Biological Process | carbohydrate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameIntracellular maltogenic amylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionO06988
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000366977 | 1-588 | Intracellular maltogenic amylase | |||
Sequence: MEYAAIHHQPFSTDAYSYDGRTVHIKIRTKKGDADHIRFIWGDPYEYNDGKWSANEQPMRKIAATEMHDYWFAEVVPPFRRLQYAFVVTDDHEDIFFGSSGVCPYNEKTLETIHYYFKFPFVHEADTFQAPEWVKSTVWYQIFPERFANGREDLSPKNALPWGSKDPDVNDFFGGDLQGIVDKLDYLEDLGVNGIYLTPIFSAPSNHKYDTLDYFSIDPHFGDPELFRTLVSQLHQRGMRIMLDAVFNHIGSASPQWQDVVKNGDQSRYKDWFHIHSFPVTDDNYDRFAFTADMPKLNTANPEVQKYLLDIALYWIREFDIDGWRLDVANEVDHVFWKTFRQAVSTEKPDVYILGEIWHSAEPWLRGDEFHAAMNYPFTEPMIEYFADQTISASRMAHRVNAHLMNGMKQANEVMFNLLDSHDTKRLLTRCRNDEKKARALLAFMFAQTGSPCIYYGTEIGLNGENDPLCRKCMVWEKEKQNQDMLQFMKRLIALRKQENTLLTEGHLEWNLLDDKNDFISFSRTLDEKILIYFFNQGNVVQHVSLRELNIDRNKKICDAWTEQPLQHHDVIAVQPGEFLILSAAAPV |
Proteomic databases
Interaction
Structure
Family & Domains
Sequence similarities
Belongs to the glycosyl hydrolase 13 family. BbmA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length588
- Mass (Da)68,722
- Last updated2009-03-24 v2
- Checksum6132681370919DFE
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z94043 EMBL· GenBank· DDBJ | CAB08035.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AL009126 EMBL· GenBank· DDBJ | CAB15467.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |