O06825 · LDT3_MYCTU
- ProteinProbable L,D-transpeptidase 3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids271 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Probable L,D-transpeptidase that may perform as-yet-unknown cross-linking reactions in M.tuberculosis. Is not able to generate 3->3 cross-links in peptidoglycan, using tetrapeptide stems as acyl donor substrates. May function in the anchoring of proteins to peptidoglycan.
Activity regulation
Is irreversibly inactivated by the beta-lactams carbapenems via the formation of a covalent adduct resulting from enzyme acylation; imipenem is the most efficient drug for in vitro LdtMt3 inactivation.
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 228 | Proton donor/acceptor | ||||
Sequence: H | ||||||
Active site | 246 | Nucleophile | ||||
Sequence: C | ||||||
Site | 246 | Binds to carbapenem drug (covalent) | ||||
Sequence: C |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | acyltransferase activity | |
Molecular Function | peptidoglycan L,D-transpeptidase activity | |
Biological Process | cell wall organization | |
Biological Process | peptidoglycan-protein cross-linking | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable L,D-transpeptidase 3
- EC number
- Short namesLDT 3
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionO06825
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000430335 | 1-271 | Probable L,D-transpeptidase 3 | |||
Sequence: MRAVFGCAIAVVGIAGSVVAGPADIHLVAAKQSYGFAVASVLPTRGQVVGVAHPVVVTFSAPITNPANRHAAERAVEVKSTPAMTGKFEWLDNDVVQWVPDRFWPAHSTVELSVGSLSSDFKTGPAVVGVASISQHTFTVSIDGVEEGPPPPLPAPHHRVHFGEDGVMPASMGRPEYPTPVGSYTVLSKERSVIMDSSSVGIPVDDPDGYRLSVDYAVRITSRGLYVHSAPWALPALGLENVSHGCISLSREDAEWYYNAVDIGDPVIVQE |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 127-270 | L,D-TPase catalytic | ||||
Sequence: VVGVASISQHTFTVSIDGVEEGPPPPLPAPHHRVHFGEDGVMPASMGRPEYPTPVGSYTVLSKERSVIMDSSSVGIPVDDPDGYRLSVDYAVRITSRGLYVHSAPWALPALGLENVSHGCISLSREDAEWYYNAVDIGDPVIVQ |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length271
- Mass (Da)28,701
- Last updated1997-07-01 v1
- Checksum719301725EAC509A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP003248 EMBL· GenBank· DDBJ | AFN49342.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL123456 EMBL· GenBank· DDBJ | CCP44192.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JLDD01000016 EMBL· GenBank· DDBJ | KBJ35648.1 EMBL· GenBank· DDBJ | Genomic DNA |