O04719 · P2C77_ARATH

Function

function

Repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), high light stress, response to glucose, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as H2O2 and oxidative burst in response to ABA and dehydration. Represses GHR1 and, to some extent, SRK2E/OST1, kinases involved in the regulation of SLAC1-dependent stomatal closure (PubMed:22730405).
Controls negatively fibrillin that is involved in mediating ABA-induced photoprotection. May be implicated in ABA content regulation. Involved in acquired thermotolerance of root growth and seedling survival. Required for the Erwinia amylovora harpin-induced (HrpN) drought tolerance. Involved in the hydrotropic response

Miscellaneous

The reduced form of ABI2 is converted to the oxidized form by the addition of oxidized GPX3 or H2O2.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 magnesium or manganese ions per subunit.

Activity regulation

Phosphatase activity repressed by oxidized ATGPX3, free fatty acids (e.g. arachidonic acid (20:4) and Linolenic acid (18:3)) and by H2O2. Repressed by PYR/PYL/RCAR ABA receptors in an ABA-dependent manner.

pH Dependence

Optimum pH is 8.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site165Mg2+ 1 (UniProtKB | ChEBI)
Binding site165Mg2+ 2 (UniProtKB | ChEBI)
Binding site251Mg2+ 1 (UniProtKB | ChEBI)
Binding site252Mg2+ 1 (UniProtKB | ChEBI)
Site290Lock
Binding site337Mg2+ 1 (UniProtKB | ChEBI)
Binding site337Mg2+ 2 (UniProtKB | ChEBI)
Binding site402Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionmetal ion binding
Molecular Functionmyosin phosphatase activity
Molecular Functionprotein serine/threonine phosphatase activity
Biological Processabscisic acid-activated signaling pathway
Biological Processnegative regulation of protein kinase activity
Biological Processphotoinhibition
Biological Processregulation of stomatal opening
Biological Processresponse to abscisic acid
Biological Processresponse to heat
Biological Processresponse to osmotic stress
Biological Processresponse to water deprivation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein phosphatase 2C 77
  • EC number
  • Short names
    AtPP2C77
  • Alternative names
    • Protein ABSCISIC ACID-INSENSITIVE 2
    • Protein phosphatase 2C ABI2
      (PP2C ABI2
      )

Gene names

    • Name
      ABI2
    • Synonyms
      PP2C77
    • ORF names
      MHM17.19
    • Ordered locus names
      At5g57050

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia
    • cv. Landsberg erecta
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    O04719
  • Secondary accessions
    • B3H561

Proteomes

Organism-specific databases

Genome annotation databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis168In abi2; reduced phosphatase activity, reduced affinity with magnesium ions, loss of interaction with the fibrillin precursor protein, impaired ABA-mediated binding to PYR1, and reduced negative control on fibrillin activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 40 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00000577671-423Protein phosphatase 2C 77
Disulfide bond257↔331

Keywords

Proteomic databases

PTM databases

Expression

Induction

Repressed by MYB44 and ERF4. Induced by salt stress and ABA.

Gene expression databases

Interaction

Subunit

Interacts with SPK1, CIPK15/PKS3, GPX3, SCAR1, SCAR2, SCAR3 and SCARL. Interacts also with CIPK24/SOS2. Binds to the fibrillin precursor protein. Interacts with ABA-bounded PYR1, PYL1, PYL2, PYL3, PYL4, PYL5, PYL6, PYL8 and PYL9, and with free PYL2, PYL3 and PYL4. Interacts with and represses GHR1, and, to a lesser extent, SRK2E/OST1 (PubMed:22730405).

Binary interactions

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region74-95Disordered
Compositional bias79-95Basic and acidic residues
Domain112-411PPM-type phosphatase

Domain

The 'lock' site stabilizes the complex made of PP2C, ABA and PYR/PYL/RCAR receptor by keeping receptor 'gate' and 'latch' loops in closed positions.

Sequence similarities

Belongs to the PP2C family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

O04719-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    423
  • Mass (Da)
    46,306
  • Last updated
    1997-07-01 v1
  • Checksum
    67CAAC76DA531A71
MDEVSPAVAVPFRPFTDPHAGLRGYCNGESRVTLPESSCSGDGAMKDSSFEINTRQDSLTSSSSAMAGVDISAGDEINGSDEFDPRSMNQSEKKVLSRTESRSLFEFKCVPLYGVTSICGRRPEMEDSVSTIPRFLQVSSSSLLDGRVTNGFNPHLSAHFFGVYDGHGGSQVANYCRERMHLALTEEIVKEKPEFCDGDTWQEKWKKALFNSFMRVDSEIETVAHAPETVGSTSVVAVVFPTHIFVANCGDSRAVLCRGKTPLALSVDHKPDRDDEAARIEAAGGKVIRWNGARVFGVLAMSRSIGDRYLKPSVIPDPEVTSVRRVKEDDCLILASDGLWDVMTNEEVCDLARKRILLWHKKNAMAGEALLPAEKRGEGKDPAAMSAAEYLSKMALQKGSKDNISVVVVDLKGIRKFKSKSLN

O04719-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8BEK2A0A1P8BEK2_ARATHABI2312

Features

Showing features for alternative sequence, compositional bias.

TypeIDPosition(s)Description
Alternative sequenceVSP_03483429-68in isoform 2
Compositional bias79-95Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Y08966
EMBL· GenBank· DDBJ
CAA70163.1
EMBL· GenBank· DDBJ
Genomic DNA
Y08965
EMBL· GenBank· DDBJ
CAA70162.1
EMBL· GenBank· DDBJ
mRNA
Y11840
EMBL· GenBank· DDBJ
CAA72538.1
EMBL· GenBank· DDBJ
Genomic DNA
AB024035
EMBL· GenBank· DDBJ
BAA97035.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED96839.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED96840.1
EMBL· GenBank· DDBJ
Genomic DNA
AY136415
EMBL· GenBank· DDBJ
AAM97081.1
EMBL· GenBank· DDBJ
mRNA
BT008860
EMBL· GenBank· DDBJ
AAP68299.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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