O01803 · RB11A_CAEEL

  • Protein
    Ras-related protein rab-11.1
  • Gene
    rab-11.1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:21320697, PubMed:24843160).
Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:21320697, PubMed:24843160).
Involved in regulating the meiotic maturation of oocytes (PubMed:18472420).
Plays a role in egg shell formation, regulating exocytosis of chondroitin proteoglycans following fertilization (PubMed:18765566, PubMed:26506309).
Controls cortical granule localization and targets them to the plasma membrane for exocytosis (PubMed:18765566, PubMed:22992455).
Acts as a major regulator of membrane delivery during cytokinesis (PubMed:18765566, PubMed:20116245, PubMed:26506309).
Regulates the cytoskeleton by facilitating astral microtubule elongation and organization during metaphase to ensure proper spindle alignment and polarity in the first embryonic cell division (PubMed:18385514).
Maintains normal endoplasmic reticulum morphology during metaphase (PubMed:18385514).
Involved in vesicle formation and plasma membrane repair following exposure to pore forming toxins (PubMed:21320697).
Regulates endocytic recycling (PubMed:24843160).
May play a role in yolk receptor endocytosis in growing oocytes (PubMed:18354496, PubMed:26506309).
Plays a role in the shedding of pathogen spores from intestinal cells via its involvement in spore fusion and endocytic trafficking (PubMed:24843160).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site18-26GTP (UniProtKB | ChEBI)
Binding site66-70GTP (UniProtKB | ChEBI)
Binding site124-127GTP (UniProtKB | ChEBI)
Binding site154-156GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentapical plasma membrane
Cellular Componentcentrosome
Cellular Componentcortical granule
Cellular Componentcytosol
Cellular ComponentGolgi apparatus
Cellular ComponentGolgi membrane
Cellular ComponentGolgi trans cisterna membrane
Cellular Componentrecycling endosome
Cellular Componentrecycling endosome membrane
Cellular Componentspindle
Cellular Componentspindle pole body
Cellular Componenttransport vesicle
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Biological Processcortical granule exocytosis
Biological Processeggshell formation
Biological Processembryo development ending in birth or egg hatching
Biological Processexocytosis
Biological Processmembrane addition at site of mitotic cytokinesis
Biological Processmitotic cytokinesis
Biological Processprotein transport
Biological Processreceptor-mediated endocytosis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ras-related protein rab-11.1
  • Alternative names
    • Rab GTPase rab-11.1

Gene names

    • Name
      rab-11.1
    • ORF names
      F53G12.1

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    O01803
  • Secondary accessions
    • I7EVK5

Proteomes

Organism-specific databases

Subcellular Location

Endosome
Apical cell membrane
Cytoplasm, cytosol
Recycling endosome membrane
; Lipid-anchor
Cytoplasmic granule
Note: Expressed in endosomal vesicles which localize to the pericentriolar region of embryos during prophase and metaphase (PubMed:19158384).
Localizes along the spindle in embryos during anaphase (PubMed:19158384).
Transiently accumulates on secretory vesicles before their exocytosis (PubMed:18765566).
Localizes to recycling endosomes and Golgi apparatus membrane in growing oocytes (PubMed:18765566, PubMed:26506309).
Translocates to cytoplasmic granules and extracellular matrix components in mature oocytes (PubMed:26506309).
Accumulates on ring-shaped structures in ovulating oocytes and in early one-cell stage embryos (PubMed:18765566).
Redistributes to recycling endosomes and Golgi apparatus in embryos (PubMed:26506309).
Co-localizes with rei-1 at Golgi apparatus membrane in embryos (PubMed:26506309).
Reycling endosomes co-localize with the endoplasmic reticulum in embryos (PubMed:18385514).
Co-localizes with rab-6.1-positive vesicles near the plasma membrane until vesicle exocytosis in embryos (PubMed:22992455).
Localizes to the cytosol during the meront-stage of N.parisii infection and around the resulting spores that are to be shed from intestinal cells (PubMed:24843160).

Keywords

Phenotypes & Variants

Disruption phenotype

Zygotic lethal (PubMed:18765566).
RNAi-mediated knockdown results in a reduced meiotic maturation rate of oocytes (PubMed:18472420).
Embryos are multinucleated and osmotically sensitive with permeable and therefore defective eggshells (PubMed:18385514, PubMed:18765566).
During the first cell cycle, 52% of embryos fail to extrude polar bodies, 83% of embryos display no or minimal pseudocleavage and the centrosomal-nuclear complex does not migrate to the center of embryos (PubMed:18385514).
Animals display mitotic spindle alignment defects whereby in 73.9% of embryos, the P0 spindle does not rotate to the anterior-posterior axis of the embryo during anaphase, but during the metaphase to anaphase transition, the movements of the spindle are more abrupt with the spindle migrating to the posterior pole and then rebounding to the anterior-posterior axis (PubMed:18385514).
In 17.4% of embryos the centrosomes are unstable and the spindle is displaced further towards the posterior pole (PubMed:18385514).
Impaired localization of proteins important for establishing cell polarity such as par-2, par-3, gpr-1 and gpr-2 (PubMed:18385514).
Altered microtubule organization and dynamics during metaphase including a greater distance between the growing microtubule plus ends and the posterior cortex, impeded microtubule growth with fewer growing astral microtubules reaching the cortex, and a reduced microtubule nucleation rate (PubMed:18385514).
Slight defect in endoplasmic reticulum morphology whereby the large endoplasmic reticulum aggregates that normally form during metaphase and persist during anaphase, form, but do not persist during anaphase and disperse (PubMed:18385514).
Mild endocytosis defect with an accumulation of the yolk protein vitellogenin in the pseudocoelom (PubMed:18354496).
Impaired cortical granule (secretory vesicle) localization with granules abnormally clustered around the nuclear envelope of proximal oocytes, irregulary dispersed in the cytoplasm of oocytes and retained in the embryo following entry into the uterus (PubMed:18765566).
Cortical granule exocytosis defects whereby cav-1-positive cortical granules are not tethered to the plasma membrane, but are distributed throughout the cytoplasm and accumulate around the nucleus in embryos following fertilization (PubMed:22992455).
Disrupted fusion of spores, containing intracellular pathogen N.parisii, with the apical cell membrane and thus disrupted clearance from intestinal cells (PubMed:24843160).
RNAi-mediated knockdown in RNAi-sensitive mutants exposed to bacterial pore-forming toxin Cry5B results in intoxification and impaired plasma membrane repair (PubMed:21320697).
RNAi-mediated knockdown in the intestine results in reduced survival upon exposure to Cry5B (PubMed:21320697).

PTM/Processing

Features

Showing features for chain, lipidation.

TypeIDPosition(s)Description
ChainPRO_00004356821-211
Lipidation208S-geranylgeranyl cysteine
Lipidation209S-geranylgeranyl cysteine

Keywords

Proteomic databases

Expression

Tissue specificity

Expressed weakly in sperm, but more predominantly in oocytes (PubMed:18472420).
Expressed in the intestine (PubMed:24843160).

Developmental stage

Expressed during embryogenesis (PubMed:18385514, PubMed:20116245, PubMed:26506309).
Transiently expressed during cytokinesis in embryos on the ingressing furrow and at the midbody during early abscission (PubMed:20116245).
Expressed during the L4 stage and young adult stage of development (PubMed:22634595).

Gene expression databases

    • WBGene00004274Expressed in pharyngeal muscle cell (C elegans) and 5 other cell types or tissues

Interaction

Subunit

Interacts with rei-1 and rei-2. The GDP-form preferentially binds to rei-1 and rei-2.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif, region.

TypeIDPosition(s)Description
Motif40-48Effector region
Region187-211Disordered

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    211
  • Mass (Da)
    23,429
  • Last updated
    1997-07-01 v1
  • Checksum
    B0FD2837E42BE574
MGSRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSISVEGKTVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHVTYENVERWLKELRDHADQNIVIMLVGNKSDLRHLRAVPTDEAKIYAERNQLSFIETSALDSTNVEAAFTNILTEIYKSVSNKHVGTDRQGYGGGSGTIIPSPASDPPKKQCCIP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JQ235189
EMBL· GenBank· DDBJ
AFP33153.1
EMBL· GenBank· DDBJ
mRNA
BX284601
EMBL· GenBank· DDBJ
CCD71701.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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