O01677 · O01677_BOMMO

Function

function

Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.

Catalytic activity

  • (2E)-decenoyl-[ACP] + H+ + NADPH = decanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-dodecenoyl-[ACP] + H+ + NADPH = dodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexadecenoyl-[ACP] + H+ + NADPH = hexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexenoyl-[ACP] + H+ + NADPH = hexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-octadecenoyl-[ACP] + H+ + NADPH = NADP+ + octadecanoyl-[ACP]
    This reaction proceeds in the forward direction.
  • (2E)-octenoyl-[ACP] + H+ + NADPH = NADP+ + octanoyl-[ACP]
    This reaction proceeds in the forward direction.
  • (2E)-tetradecenoyl-[ACP] + H+ + NADPH = NADP+ + tetradecanoyl-[ACP]
    This reaction proceeds in the forward direction.
  • (3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • 3-oxobutanoyl-[ACP] + H+ + NADPH = (3R)-hydroxybutanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxodecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxydecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxododecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxydodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexadecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyhexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyhexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctadecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyoctadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyoctanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxotetradecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxytetradecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • H+ + hexadecanoyl-[ACP] + malonyl-[ACP] = 3-oxooctadecanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • H+ + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • H+ + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • H+ + malonyl-[ACP] + tetradecanoyl-[ACP] = 3-oxohexadecanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • H2O + hexadecanoyl-[ACP] = H+ + hexadecanoate + holo-[ACP]
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • H2O + tetradecanoyl-[ACP] = H+ + holo-[ACP] + tetradecanoate
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] + NADP+ = a 3-oxoacyl-[ACP] + H+ + NADPH
    This reaction proceeds in the backward direction.
    EC:1.1.1.100 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
    EC:4.2.1.59 (UniProtKB | ENZYME | Rhea)
  • a 2,3-saturated acyl-[ACP] + NADP+ = a (2E)-enoyl-[ACP] + H+ + NADPH
    This reaction proceeds in the backward direction.
    EC:1.3.1.39 (UniProtKB | ENZYME | Rhea)
  • a fatty acyl-[ACP] + H+ + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
    EC:2.3.1.41 (UniProtKB | ENZYME | Rhea)
  • acetyl-CoA + holo-[ACP] = acetyl-[ACP] + CoA
    This reaction proceeds in the forward direction.
    EC:2.3.1.38 (UniProtKB | ENZYME | Rhea)
  • acetyl-[ACP] + H+ + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • butanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxohexanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • decanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxododecanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.
  • dodecanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxotetradecanoyl-[ACP] + CO2 + holo-[ACP]
    This reaction proceeds in the forward direction.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site906Proton acceptor; for dehydratase activity
Active site1056Proton donor; for dehydratase activity

GO annotations

AspectTerm
Molecular Function(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] synthase activity
Molecular Function[acyl-carrier-protein] S-acetyltransferase activity
Molecular Functionenoyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Functionfatty acid synthase activity
Molecular Functionfatty acyl-[ACP] hydrolase activity
Biological Processfatty acid biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Submitted names
    • p260

Gene names

    • Name
      693037

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • Kin-shu x Sho-wa
    • p50T
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Lepidoptera > Glossata > Ditrysia > Bombycoidea > Bombycidae > Bombycinae > Bombyx

Accessions

  • Primary accession
    O01677

Proteomes

PTM/Processing

Keywords

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-25Disordered
Domain27-432Ketosynthase family 3 (KS3)
Region869-993N-terminal hotdog fold
Domain869-1133PKS/mFAS DH
Region1007-1133C-terminal hotdog fold

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,342
  • Mass (Da)
    262,354
  • Last updated
    1997-07-01 v1
  • Checksum
    A9052B8DAB9F05A9
MAPSAQRTMVNPPEVKDDGSAPKPLTGEEVVISGMSGIFPKSDSVLEFKNNLFNKVDMVTQEGSRWVFNHPEIPKHLGKVKGMSKFDAQFFRVHYKQACTMEPMSRKLLEHAYSAIYDPGINPVQLYGKKIGVFIGAAFSESEKMIIYENIQRNGFGITGGNKAMYANRISYWIDGKGPSYALDAACASSMSCLEHAYRCMSTGQCEAAIVGGCNLCAHPNVTLNCKRAGFLCLDGKTKCFDKNGDGYVRSDAVSVLFLQKSKNAKRIYSEVYYAKTNYGTNVNADEFLPIREPKEIQEFLEAFYSEIDVCPKDVEYIEASGAAIAEADGKELEAIGKVFANDKTIKVGCVKSNMGSSEAASGVCAVTKVCLAYHEGKLPANLHYSEPQDQIDSVRDGRVQVLTDNSPFKRGFSALNMFSFGGVNLHVLLKGHFKPKDLQRYKSSIPHLVLASGRQNNCVEKIFESLKNRPVDAEQIALIHDIHDYEIAGHMCRGYIILENNENNETVCLTESIEYCPGITRPVWFVYSGMGSQWAGMGAELMRIPVFAAAIEKCHKVLAPKGIDIVRILCEPDKTIFDNILHSFVGIAAVQIGLTDVLREIGIVPDHIIGHSVGELGCAYADGCFTAEEMILSAYSRGLVSVQTPFIRGSMAAVGLGYKNILPLCPPEIEVACHNSSDSSTISGPAEPMKKFISELTSKGIFAKEVPCSNISFHSRYIADGGPGLLKYLSDVIKDPKPRSEKWVSTSVPQENWDDPKAKFSSAEYHTNNLLNPVLFEETSKLIPSDSLVIEIAPHGLLQAILKRSMPECQHLPLTRRGHADPVKFLLEGIGKLYELGLNPKISALYPKIEYPVSTETPLLSHLVEWEHSETWPQAIYNTKHRVTAPCYEFVMSIHDDDFKYFRGHNREGICVLPEAAFLVNVWQTLAMYNGKNYKDMSVVFKDLKFRGEVAIRDEDTLKFSFMIHKGNARFGVSYENTEIVSGFVKEAVTEDFADRNPPQSDDTDDFALDSKDVYTLMNLRGYSYSDAFQSIHSTTSSRKKGIIKWPEDWISFLDGLIQLNAFAKDHDGVSVPTIIRKLSINVEEHKNAKMIALDNVNCINAEIYDHYNYTKCGGVELVGLTFNDKPAVEKEPDVMQSRVFVPYFVTGVVDLKTALQVSFGTISDNVNGEEVNVIECLRSEDHEISKLIKEVTRTHASANFDVVSVESLDLKAVSGADVLITVNLLGNEMEMKKIHDSLRQNSFILSMEEDISNINPDHSLFTTVSAMSVQNHILVLLRKTSEDIDVTFMPINHDINFTWVSRLRNEIEKSRKVVLISERQPYCGLYGLVKKLSKQPRNNVSLVLVDDFYHVPSFDAEHPLFKEQLRKNLTFNVLKKGVWGSFYYLSNDSYSNFQNVYLTNAITGDLNSLTWIEASSTPANNNLVQVCYSSPSYRDAQDAAGTTSKSNRSFGMDYSGINSLGERVMGVVQGGALSTLVEADPDLTWPVPEHWTLEDAATVPLPYLQAYYCLAIKSELLRKRKVFINGGAGALGQAVISICLALDCEVFTTVSDTKKKEFLMKLFPSLPESNIASSHNIHFKDRVMQNTKNKGCDVVINYMNGNLREAVIKCVAKYSLFLDLSEHDMIRNNDFHMSFLFTSGSYVSTQFSSIFKTEGLNEKKLLHKLISEGIASGVVRPLSRMVYSPANVSRAFRLLASSKHRGKVLIKMRDSNTNHDLKVHPRLTCTSRGSYIVVCDESALSLELIDRLVKRGARNLFIHIKPNSIAGYCFTKIFKWKKLNVTIKISSENLQTENGCTNMIREATKTGPVCGIFIVQNYSSDAQEDQFRANVVTDKFKNVACVVANLDLVSRNICNELNHFVIINYGPKSYADVYVGSVIDKICQSRNEITLPALVFRIGTIAELDRNLDNLQTKIKAQTLSTALNGLESSLKMKNSNTVAYNLKKRSDLSFIERVSRIIGVENINEIKGTHTLQDITNDVAIQQIITAIREEYNILYPDEQVKKMTIASIKNFESRMVKANNNFNGGLGAFYTFIDEDECYATEPVIPMRTKFSIVKEEEELDPKATYLFLVPGFEGHNRIFTSICERLKVQAMTLQLGPDVMDDTIPQMAYNIRKFMKKRFELKSKFYLLGYSFGVNVALELAALLEKEGHVGVVYCLDSSPDAMRAQLDAYIGNLKDTELQDRIVEHMYKLMAGTDSEELKEELRKTEDWPDKVEACINRLKGVVHYSHQYKRSILQGAYRRILLAREYEPKFQLESELVLMKGIPHPSMGYLANDYNLFKYSKRPVQVFKLESDHALAPQDCRASNIINKLLDPAVLEEFNKSNLCESYLADPTNIL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U67866
EMBL· GenBank· DDBJ
AAB53257.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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