O01677 · O01677_BOMMO
- Proteinp260
- Gene693037
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids2342 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
Catalytic activity
- (2E)-butenoyl-[ACP] + H+ + NADPH = butanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-decenoyl-[ACP] + H+ + NADPH = decanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-dodecenoyl-[ACP] + H+ + NADPH = dodecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-hexadecenoyl-[ACP] + H+ + NADPH = hexadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-hexenoyl-[ACP] + H+ + NADPH = hexanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-octadecenoyl-[ACP] + H+ + NADPH = NADP+ + octadecanoyl-[ACP]This reaction proceeds in the forward direction.
- (2E)-octenoyl-[ACP] + H+ + NADPH = NADP+ + octanoyl-[ACP]This reaction proceeds in the forward direction.
- (2E)-tetradecenoyl-[ACP] + H+ + NADPH = NADP+ + tetradecanoyl-[ACP]This reaction proceeds in the forward direction.
- (3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- 3-oxobutanoyl-[ACP] + H+ + NADPH = (3R)-hydroxybutanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxodecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxydecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxododecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxydodecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxohexadecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyhexadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxohexanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyhexanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxooctadecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyoctadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxooctanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyoctanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxotetradecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxytetradecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- H+ + hexadecanoyl-[ACP] + malonyl-[ACP] = 3-oxooctadecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- H+ + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- H+ + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- H+ + malonyl-[ACP] + tetradecanoyl-[ACP] = 3-oxohexadecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- acetyl-[ACP] + H+ + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- butanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxohexanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- decanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxododecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- dodecanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxotetradecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 906 | Proton acceptor; for dehydratase activity | ||||
Sequence: H | ||||||
Active site | 1056 | Proton donor; for dehydratase activity | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity | |
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity | |
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] synthase activity | |
Molecular Function | [acyl-carrier-protein] S-acetyltransferase activity | |
Molecular Function | enoyl-[acyl-carrier-protein] reductase (NADPH) activity | |
Molecular Function | fatty acid synthase activity | |
Molecular Function | fatty acyl-[ACP] hydrolase activity | |
Biological Process | fatty acid biosynthetic process |
Keywords
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Submitted names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Lepidoptera > Glossata > Ditrysia > Bombycoidea > Bombycidae > Bombycinae > Bombyx
Accessions
- Primary accessionO01677
Proteomes
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-25 | Disordered | ||||
Sequence: MAPSAQRTMVNPPEVKDDGSAPKPL | ||||||
Domain | 27-432 | Ketosynthase family 3 (KS3) | ||||
Sequence: GEEVVISGMSGIFPKSDSVLEFKNNLFNKVDMVTQEGSRWVFNHPEIPKHLGKVKGMSKFDAQFFRVHYKQACTMEPMSRKLLEHAYSAIYDPGINPVQLYGKKIGVFIGAAFSESEKMIIYENIQRNGFGITGGNKAMYANRISYWIDGKGPSYALDAACASSMSCLEHAYRCMSTGQCEAAIVGGCNLCAHPNVTLNCKRAGFLCLDGKTKCFDKNGDGYVRSDAVSVLFLQKSKNAKRIYSEVYYAKTNYGTNVNADEFLPIREPKEIQEFLEAFYSEIDVCPKDVEYIEASGAAIAEADGKELEAIGKVFANDKTIKVGCVKSNMGSSEAASGVCAVTKVCLAYHEGKLPANLHYSEPQDQIDSVRDGRVQVLTDNSPFKRGFSALNMFSFGGVNLHVLLKG | ||||||
Region | 869-993 | N-terminal hotdog fold | ||||
Sequence: HSETWPQAIYNTKHRVTAPCYEFVMSIHDDDFKYFRGHNREGICVLPEAAFLVNVWQTLAMYNGKNYKDMSVVFKDLKFRGEVAIRDEDTLKFSFMIHKGNARFGVSYENTEIVSGFVKEAVTED | ||||||
Domain | 869-1133 | PKS/mFAS DH | ||||
Sequence: HSETWPQAIYNTKHRVTAPCYEFVMSIHDDDFKYFRGHNREGICVLPEAAFLVNVWQTLAMYNGKNYKDMSVVFKDLKFRGEVAIRDEDTLKFSFMIHKGNARFGVSYENTEIVSGFVKEAVTEDFADRNPPQSDDTDDFALDSKDVYTLMNLRGYSYSDAFQSIHSTTSSRKKGIIKWPEDWISFLDGLIQLNAFAKDHDGVSVPTIIRKLSINVEEHKNAKMIALDNVNCINAEIYDHYNYTKCGGVELVGLTFNDKPAVEKE | ||||||
Region | 1007-1133 | C-terminal hotdog fold | ||||
Sequence: DFALDSKDVYTLMNLRGYSYSDAFQSIHSTTSSRKKGIIKWPEDWISFLDGLIQLNAFAKDHDGVSVPTIIRKLSINVEEHKNAKMIALDNVNCINAEIYDHYNYTKCGGVELVGLTFNDKPAVEKE |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,342
- Mass (Da)262,354
- Last updated1997-07-01 v1
- ChecksumA9052B8DAB9F05A9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U67866 EMBL· GenBank· DDBJ | AAB53257.1 EMBL· GenBank· DDBJ | mRNA |