O01658 · NO66_CAEEL
- ProteinBifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
- Genejmjc-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids748 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code (By similarity).
Mediates response to multiple stress stimuli, including heat shock and osmotic, oxidative, and ethanol stress
Mediates response to multiple stress stimuli, including heat shock and osmotic, oxidative, and ethanol stress
Catalytic activity
- 2 2-oxoglutarate + N6,N6-dimethyl-L-lysyl36-[histone H3] + 2 O2 = 2 CO2 + 2 formaldehyde + L-lysyl36-[histone H3] + 2 succinate
2 CHEBI:16810 + RHEA-COMP:9787 CHEBI:61976 Position: 36+ 2 CHEBI:15379 = 2 CHEBI:16526 + 2 CHEBI:16842 + RHEA-COMP:9785 CHEBI:29969 Position: 36+ 2 CHEBI:30031
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleolus | |
Cellular Component | nucleus | |
Molecular Function | 2-oxoglutarate-dependent dioxygenase activity | |
Molecular Function | histone H3K36 demethylase activity | |
Molecular Function | histone H3K36me/H3K36me2 demethylase activity | |
Molecular Function | histone H3K4 demethylase activity | |
Molecular Function | histone H3K4me/H3K4me2/H3K4me3 demethylase activity | |
Molecular Function | iron ion binding | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | response to ethanol | |
Biological Process | response to heat | |
Biological Process | response to osmotic stress | |
Biological Process | response to oxidative stress |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionO01658
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Attenuated response to heat shock stress and reduced survival after heat shock and oxidative stress.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000390981 | 1-748 | Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 | |||
Sequence: MGKKKNSNKSAAAAPAVKHNDRWSSIELGEAKSAAVSHYKEPSKEPKFVHPAKLEKVKRIHDGLNIDRVLSHGPVPKQNGGTKRKHVEVTTQKLENKKPKVEVKKEDEKSKNKKMKNQNKHTALVQNETSTRSTYFVEEPDNENKVTLISNGREIAFKKTEVVESDDEQMIGLDSDEELEDEDETDIDEDEMMIDPKDIERYINFESVEDEEDMEDEEIEDEEFEDEEFEDEEEEADEQEEEEEDVSDEESVVSEMDADSDDEGFIAGKDREAHVISKDKFTRNAPAVDFDKFPFTDEDSVVTSSRAFGFMISPCDVQTFFDKFYQSNVLVVRRKQPTYFGNLFSTARLGELLEKNHLEYGRNINIAQYKNGVRTTLNGQGRAYPQIVKQHLHNMCSVQLVNPQTYDDRIWYLCEVIQEQFGCFVGANTYLTPAGSSGFAPHWDEIDAFLLQVEGRKYWRVWAPESAEEELPLESSDNFTEDDMKGREPVFEGWIEKGDMIYIPRGYIHQARTDSKVHSLHVTVSTGRQWSFANLMEKVVPEAIGVLTDTRHKLRRGLPTGLFDMGGVIDLDYSQEDHFVEKFKMVVDRHMSMLRNLVADQLLESSVDSLAKEFMKQALPPRLTEQEKKLSVLGSSTNLLGDDLVDFTARTKVRLIRRHTQRLLMESEDACFISHRINNSRLFEGRPEQIVEYPISGIDAYRVLSNSYPEWRTLYEIFSLRETKTKSRKENLAAIQLLFQIGVLLVKN |
Proteomic databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 65-135 | Disordered | ||||
Sequence: NIDRVLSHGPVPKQNGGTKRKHVEVTTQKLENKKPKVEVKKEDEKSKNKKMKNQNKHTALVQNETSTRSTY | ||||||
Compositional bias | 81-114 | Basic and acidic residues | ||||
Sequence: GTKRKHVEVTTQKLENKKPKVEVKKEDEKSKNKK | ||||||
Compositional bias | 119-135 | Polar residues | ||||
Sequence: NKHTALVQNETSTRSTY | ||||||
Region | 160-264 | Disordered | ||||
Sequence: TEVVESDDEQMIGLDSDEELEDEDETDIDEDEMMIDPKDIERYINFESVEDEEDMEDEEIEDEEFEDEEFEDEEEEADEQEEEEEDVSDEESVVSEMDADSDDEG | ||||||
Compositional bias | 167-193 | Acidic residues | ||||
Sequence: DEQMIGLDSDEELEDEDETDIDEDEMM | ||||||
Compositional bias | 205-261 | Acidic residues | ||||
Sequence: FESVEDEEDMEDEEIEDEEFEDEEFEDEEEEADEQEEEEEDVSDEESVVSEMDADSD | ||||||
Domain | 399-543 | JmjC | ||||
Sequence: QLVNPQTYDDRIWYLCEVIQEQFGCFVGANTYLTPAGSSGFAPHWDEIDAFLLQVEGRKYWRVWAPESAEEELPLESSDNFTEDDMKGREPVFEGWIEKGDMIYIPRGYIHQARTDSKVHSLHVTVSTGRQWSFANLMEKVVPEA |
Sequence similarities
Belongs to the ROX family. NO66 subfamily.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
O01658-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length748
- Mass (Da)86,026
- Last updated2001-10-01 v2
- ChecksumA3E1587BAAB5E3AA
O01658-2
- Nameb
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 81-114 | Basic and acidic residues | ||||
Sequence: GTKRKHVEVTTQKLENKKPKVEVKKEDEKSKNKK | ||||||
Compositional bias | 119-135 | Polar residues | ||||
Sequence: NKHTALVQNETSTRSTY | ||||||
Alternative sequence | VSP_053839 | 121-125 | in isoform b | |||
Sequence: HTALV → RTAAS | ||||||
Alternative sequence | VSP_053840 | 126-748 | in isoform b | |||
Sequence: Missing | ||||||
Compositional bias | 167-193 | Acidic residues | ||||
Sequence: DEQMIGLDSDEELEDEDETDIDEDEMM | ||||||
Compositional bias | 205-261 | Acidic residues | ||||
Sequence: FESVEDEEDMEDEEIEDEEFEDEEFEDEEEEADEQEEEEEDVSDEESVVSEMDADSD |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FO080999 EMBL· GenBank· DDBJ | CCD68363.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FO080999 EMBL· GenBank· DDBJ | CCD68364.1 EMBL· GenBank· DDBJ | Genomic DNA |