O00219 · HYAS3_HUMAN

  • Protein
    Hyaluronan synthase 3
  • Gene
    HAS3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

The enzymatic activity depends on the availability of cytosolic levels of UDP-GlcUA and UDP-GlcNAc.

Pathway

Glycan biosynthesis; hyaluronan biosynthesis.

GO annotations

AspectTerm
Cellular Componentearly endosome
Cellular ComponentGolgi apparatus
Cellular ComponentGolgi membrane
Cellular Componenthyaluranon cable
Cellular Componentmembrane
Cellular Componentnucleus
Cellular Componentplasma membrane
Molecular Functionhyaluronan synthase activity
Molecular Functionidentical protein binding
Biological Processcarbohydrate metabolic process
Biological Processextracellular matrix assembly
Biological Processextracellular polysaccharide biosynthetic process
Biological Processhyaluronan biosynthetic process
Biological Processpositive regulation of DNA-templated transcription
Biological Processpositive regulation of hyaluranon cable assembly

Keywords

Enzyme and pathway databases

Protein family/group databases

    • GT2Glycosyltransferase Family 2

Names & Taxonomy

Protein names

  • Recommended name
    Hyaluronan synthase 3
  • EC number
  • Alternative names
    • Hyaluronate synthase 3
    • Hyaluronic acid synthase 3 (HA synthase 3)

Gene names

    • Name
      HAS3

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    O00219
  • Secondary accessions
    • A8K5T5
    • Q8WTZ0
    • Q9NYP0

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Golgi apparatus membrane
; Multi-pass membrane protein
Golgi apparatus, trans-Golgi network membrane
; Multi-pass membrane protein
Early endosome
Note: Travels from endoplasmic reticulum (ER), Golgi to plasma membrane (PubMed:26883802).
Actives only when present in plasma membrane (By similarity).
O-GlcNAcylation controls its membrane localization (PubMed:26883802).
A rapid recycling of HAS3 between plasma membrane and endosomes is controlled by the cytosolic levels of UDP-GlcUA and UDP-GlcNAc (PubMed:26883802).

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-15Cytoplasmic
Transmembrane16-36Helical; Name=1
Topological domain37-44Extracellular
Transmembrane45-65Helical; Name=2
Topological domain66-377Cytoplasmic
Transmembrane378-398Helical; Name=3
Topological domain399-408Extracellular
Transmembrane409-429Helical; Name=4
Topological domain430-440Cytoplasmic
Transmembrane441-461Helical; Name=5
Topological domain462-473Extracellular
Transmembrane474-494Helical; Name=6
Topological domain495-515Cytoplasmic
Transmembrane516-536Helical; Name=7
Topological domain537-553Extracellular

Keywords

Disease & Variants

Features

Showing features for natural variant.

TypeIDPosition(s)Description
Natural variantVAR_049317173in dbSNP:rs2232229

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 505 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Organism-specific databases

Miscellaneous

Genetic variation databases

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00001971781-553Hyaluronan synthase 3
Glycosylation462N-linked (GlcNAc...) asparagine

Post-translational modification

O-GlcNAcylation increases the hyaluronan synthase activity, HAS3 stability and its plasma membrane residence. The concentration of UDP-GlcNAc controls the level of O-GlcNAc modification.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Organism-specific databases

Interaction

Subunit

Homodimers (PubMed:25795779).
Forms heterodimers with HAS2 and HAS1 (PubMed:25795779).

Binary interactions

View interactors in UniProtKB
View CPX-8410 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Sequence similarities

Belongs to the NodC/HAS family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

O00219-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    553
  • Mass (Da)
    62,998
  • Last updated
    2008-09-23 v3
  • Checksum
    82F3B0C932EE9EA3
MPVQLTTALRVVGTSLFALAVLGGILAAYVTGYQFIHTEKHYLSFGLYGAILGLHLLIQSLFAFLEHRRMRRAGQALKLPSPRRGSVALCIAAYQEDPDYLRKCLRSAQRISFPDLKVVMVVDGNRQEDAYMLDIFHEVLGGTEQAGFFVWRSNFHEAGEGETEASLQEGMDRVRDVVRASTFSCIMQKWGGKREVMYTAFKALGDSVDYIQVCDSDTVLDPACTIEMLRVLEEDPQVGGVGGDVQILNKYDSWISFLSSVRYWMAFNVERACQSYFGCVQCISGPLGMYRNSLLQQFLEDWYHQKFLGSKCSFGDDRHLTNRVLSLGYRTKYTARSKCLTETPTKYLRWLNQQTRWSKSYFREWLYNSLWFHKHHLWMTYESVVTGFFPFFLIATVIQLFYRGRIWNILLFLLTVQLVGIIKATYACFLRGNAEMIFMSLYSLLYMSSLLPAKIFAIATINKSGWGTSGRKTIVVNFIGLIPVSIWVAVLLGGLAYTAYCQDLFSETELAFLVSGAILYGCYWVALLMLYLAIIARRCGKKPEQYSLAFAEV

O00219-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 247-553: ILNKYDSWISFLSSVRYWMAFNVERACQSYFGCVQCISGPLGMYRNSLLQQFLEDWYHQKFLGSKCSFGDDRHLTNRVLSLGYRTKYTARSKCLTETPTKYLRWLNQQTRWSKSYFREWLYNSLWFHKHHLWMTYESVVTGFFPFFLIATVIQLFYRGRIWNILLFLLTVQLVGIIKATYACFLRGNAEMIFMSLYSLLYMSSLLPAKIFAIATINKSGWGTSGRKTIVVNFIGLIPVSIWVAVLLGGLAYTAYCQDLFSETELAFLVSGAILYGCYWVALLMLYLAIIARRCGKKPEQYSLAFAEV → PPGKGMAVEDDQVQAAQVRATEAWSVHQRHVSREQ

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
H3BRH5H3BRH5_HUMANHAS3264

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_042022247-553in isoform 2
Sequence conflict493in Ref. 1; AAF36984

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF232772
EMBL· GenBank· DDBJ
AAF36984.1
EMBL· GenBank· DDBJ
mRNA
AK291400
EMBL· GenBank· DDBJ
BAF84089.1
EMBL· GenBank· DDBJ
mRNA
AC009027
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH471092
EMBL· GenBank· DDBJ
EAW83247.1
EMBL· GenBank· DDBJ
Genomic DNA
CH471092
EMBL· GenBank· DDBJ
EAW83248.1
EMBL· GenBank· DDBJ
Genomic DNA
BC021853
EMBL· GenBank· DDBJ
AAH21853.1
EMBL· GenBank· DDBJ
mRNA
U86409
EMBL· GenBank· DDBJ
AAC51209.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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