N9U4L4 · N9U4L4_9GAMM
- ProteinPhosphoribosylformylglycinamidine synthase
- GenepurL
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1296 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
Catalytic activity
- ATP + H2O + L-glutamine + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N1-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H+ + L-glutamate + phosphate
Pathway
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 305-316 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GAATGSGGEIRD | ||||||
Binding site | 676 | ATP (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 677 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 716 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 720 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 884 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 886 | ATP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 1135 | Nucleophile | ||||
Sequence: C | ||||||
Active site | 1260 | |||||
Sequence: H | ||||||
Active site | 1262 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoribosylformylglycinamidine synthase activity | |
Biological Process | 'de novo' IMP biosynthetic process | |
Biological Process | glutamine metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoribosylformylglycinamidine synthase
- EC number
- Short namesFGAM synthase ; FGAMS
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Aeromonadales > Aeromonadaceae > Aeromonas
Accessions
- Primary accessionN9U4L4
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 35-148 | Phosphoribosylformylglycinamidine synthase N-terminal | ||||
Sequence: EFMHFAELVDPLSPDERATLDRLLRYGPTIPEHAPQGHLFLVTPRPGTISPWSSKATDIAHNCGLTKVKRLERGVAYYVEGQVTDANRGQIAALLHDRMMETVFDAMGAASALF | ||||||
Domain | 169-218 | Phosphoribosylformylglycinamidine synthase linker | ||||
Sequence: LAQANLSLGLALADDEIDYLVANFQRLGRNPNDIELYMFAQANSEHCRHK | ||||||
Region | 300-325 | Disordered | ||||
Sequence: ISPFPGAATGSGGEIRDEGATGRGAK | ||||||
Domain | 430-587 | PurM-like C-terminal | ||||
Sequence: AGSALIVLGGPAMNIGLGGGAASSMTSGQSAEDLDFASVQRDNPEMERRCQEVIDRCWQLGDDNPILFIHDVGAGGLSNAMPELVSDGGRGGRFDLRAIPNDEPGMSPLEIWCNESQERYVLAVAQDKLPLFKVLCERERAPYAVIGTATEEPHLTLS | ||||||
Domain | 833-966 | PurM-like C-terminal | ||||
Sequence: HGKQRLGGSALAQVYRQLGQSAPDLDNPVLLKGFFNAIQALVSDGRLLAYHDRSDGGLLVTLLEMAFAGHCGLNIELDRVGGEVLPALFNEELGAVIQVRREDKEAVMTLLAGHGLAACAHVIGTVTSGDEIVL |
Sequence similarities
In the N-terminal section; belongs to the FGAMS family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,296
- Mass (Da)140,570
- Last updated2013-06-26 v1
- ChecksumB0AABAAB63D11AB0
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
APVG01000005 EMBL· GenBank· DDBJ | ENY73299.1 EMBL· GenBank· DDBJ | Genomic DNA |