N9U269 · N9U269_9GAMM

Function

function

Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

pyridoxal 5'-phosphate (UniProtKB | Rhea| CHEBI:597326 )

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.

GO annotations

AspectTerm
Molecular FunctionL-serine ammonia-lyase activity
Molecular Functionpyridoxal phosphate binding
Molecular Functionthreonine deaminase activity
Biological Processisoleucine biosynthetic process
Biological ProcessL-serine catabolic process
Biological Processthreonine catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    L-threonine dehydratase
  • EC number
  • Alternative names
    • Threonine deaminase

Gene names

    • Name
      ilvA
    • ORF names
      G114_07775

Organism names

  • Taxonomic identifier
  • Strain
    • 2478-85
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Aeromonadales > Aeromonadaceae > Aeromonas

Accessions

  • Primary accession
    N9U269

Proteomes

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain330-402ACT-like
Domain425-496ACT-like

Sequence similarities

Belongs to the serine/threonine dehydratase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    508
  • Mass (Da)
    56,009
  • Last updated
    2013-06-26 v1
  • Checksum
    7908C1FA319047ED
MVSAAEYLRKILLSPVYEAARVTPLQPLKKLSERLGNHISLKREDLQPVHSFKLRGAYHKIATLSAEQKARGVIAASAGNHAQGVALSAAKLGIRALIVMPTTTPDIKVDAVRRHGGNVLLHGNNFDEAYAESRRLAELERYTLIPPFDDVEVIAGQGTIGKELLEQDTHLTHVFVPVGGGGLAAGVAVYLKQLLPDIQVIGVEADGSACLKAAMTAGKPVSLDRVSLFADGVAVRRIGEETFRLCDQYLDEVVTVSNDQICAALKDIFDDCRAIAEPSGALSLAGLKAYCEREQVQGGRMAAILSGANVNFHSLRYVSERCEIGEKREGMLAVTIPERKGAFLDFCRNLGPRMVTEFNYRYSDAEQAALFVSVRLTGGEVELGQIERQLEESGYPVVNMTESELAKNHVRYMIGGRPARALHERLYSFKFPEQPGALMRFLETLGCRWNISLFHYRNHGADYGRVLCAFELPDEDVAAFHDYLREIGYAWKEVSEDPAYRLFLASGR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
APVG01000016
EMBL· GenBank· DDBJ
ENY72415.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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