N8Y4C8 · N8Y4C8_9GAMM
- ProteinRibosomal RNA large subunit methyltransferase J
- GenerlmJ
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids285 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically methylates the adenine in position 2030 of 23S rRNA.
Catalytic activity
- adenosine2030 in 23S rRNA + S-adenosyl-L-methionine = N6-methyladenosine2030 in 23S rRNA + S-adenosyl-L-homocysteine + H+
Features
Showing features for site, binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 3 | Interaction with substrate rRNA | ||||
Sequence: Y | ||||||
Binding site | 18 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 41 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 104 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 122 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 146-147 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DA | ||||||
Active site | 167 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 167 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 23S rRNA (adenine(2030)-N(6))-methyltransferase activity | |
Molecular Function | RNA binding | |
Biological Process | rRNA base methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibosomal RNA large subunit methyltransferase J
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Moraxellales > Moraxellaceae > Acinetobacter
Accessions
- Primary accessionN8Y4C8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Structure
Sequence
- Sequence statusComplete
- Length285
- Mass (Da)32,726
- Last updated2013-06-26 v1
- Checksum3F1ACA2E1AE5646D
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
APPN01000083 EMBL· GenBank· DDBJ | ENV31521.1 EMBL· GenBank· DDBJ | Genomic DNA |