N6UI18 · N6UI18_9HYPH

  • Protein
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • Gene
    ispH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site18[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site47(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site47dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site47isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site83(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site83dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site83isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site105[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site133(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site133dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site133isopentenyl diphosphate (UniProtKB | ChEBI)
Active site135Proton donor
Binding site174(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site204[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site232(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site232dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site232isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site233(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site233dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site233isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site234(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site234dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site234isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site277(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site277dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site277isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      BBbe_03790

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 91-4
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Bartonellaceae > Bartonella

Accessions

  • Primary accession
    N6UI18

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the IspH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    341
  • Mass (Da)
    37,787
  • Last updated
    2013-06-26 v1
  • Checksum
    8E1350C224E2A53E
MCLLPPLTMRLCSPRGFCAGVDRAIQIVVLALKKYGTPVYVRHEIVHNRYVVEGLQQRGAIFIEELNEIPEEHRGQPVVFSAHGVPKSVPEEAHHYNLFYIDATCPLVSKVHKQAIRHQRHGRHVILIGHSGHPEVIGTMGQLEKGAVTLIETVEDAWRYKPENPKKLGFVTQTTLSVENTAEILDVLQKRFPALETPAAESICYATTNRQEAVKAAALGSDLFLIVGAPNSSNSRRLVEVAERFGAQRSILVQCADEIDFDNLGVLSVVSLSAGASAPEIIVDEIIAAFHKRYDVKIELAETVLETETFLVNRGLRDVVLTPQDMAFVNGYAKDTNKRKL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AGWA01000006
EMBL· GenBank· DDBJ
ENN92074.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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