N6UF83 · N6UF83_9HYPH

  • Protein
    Thymidylate synthase
  • Gene
    thyA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site21dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site51(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site126-127dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Active site146
Active site146Nucleophile
Binding site166-169dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site169(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site177dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site207-209dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site263(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase

Gene names

    • Name
      thyA
    • ORF names
      BBbe_08740

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 91-4
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Bartonellaceae > Bartonella

Accessions

  • Primary accession
    N6UF83

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-264Thymidylate synthase/dCMP hydroxymethylase

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    264
  • Mass (Da)
    30,126
  • Last updated
    2013-06-26 v1
  • Checksum
    7D602D13758CE539
MKIYLDFLSHVLNSGVDRSDRTGVGTRSVFGYQMRFNLQAGFPLLTTKKLHLRSIIYELLWFLKGDTNIAWLKEHGVSIWDEWADKDGDLGPVYGFQWRSWPAPDGRYIDQISNLLDMINTNPDSRRLIVSAWNPALIDEMALPPCHCLFQFYVSDDKLSCQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKVGDFIHTFGDAHLYANHFEQAKQQLSRTPGALPVMLINPEVTDLFSFKFEDFNLLNYEAQPHIKAPVAV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AGWA01000007
EMBL· GenBank· DDBJ
ENN91179.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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