N4WHA7 · PKS2_COCH4
- ProteinReducing polyketide synthase PKS2
- GenePKS2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids2144 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Reducing polyketide synthase (PKS); part of the Tox1A locus, one of the 2 loci that mediate the biosynthesis of T-toxin, a family of linear polyketides 37 to 45 carbons in length, of which the major component is 41 carbons, and which leads to high virulence to maize (PubMed:20192833, PubMed:8953776).
One of the PKSs (PKS1 or PKS2) could synthesize a precursor, used subsequently by the other PKS as starter unit, to add additional carbons (PubMed:16529376).
Variability in the length of the final carbon backbone C35-47 could be achieved by varying the number of condensation cycles, or use of different starter or extender units or might be due to decarboxylation of the penultimate product, catalyzed by DEC1 (PubMed:12236595).
Additional proteins are required for the biosynthesis of T-toxin, including oxidoreductases RED1, RED2, RED3, LAM1 and OXI1, as well as esterase TOX9 (PubMed:20192833).
One of the PKSs (PKS1 or PKS2) could synthesize a precursor, used subsequently by the other PKS as starter unit, to add additional carbons (PubMed:16529376).
Variability in the length of the final carbon backbone C35-47 could be achieved by varying the number of condensation cycles, or use of different starter or extender units or might be due to decarboxylation of the penultimate product, catalyzed by DEC1 (PubMed:12236595).
Additional proteins are required for the biosynthesis of T-toxin, including oxidoreductases RED1, RED2, RED3, LAM1 and OXI1, as well as esterase TOX9 (PubMed:20192833).
Pathway
Mycotoxin biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 183 | For beta-ketoacyl synthase activity | |||
Active site | 319 | For beta-ketoacyl synthase activity | |||
Active site | 360 | For beta-ketoacyl synthase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] synthase activity | |
Molecular Function | fatty acid synthase activity | |
Molecular Function | oxidoreductase activity | |
Molecular Function | phosphopantetheine binding | |
Biological Process | fatty acid biosynthetic process | |
Biological Process | secondary metabolite biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReducing polyketide synthase PKS2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Pleosporomycetidae > Pleosporales > Pleosporineae > Pleosporaceae > Bipolaris
Accessions
- Primary accessionN4WHA7
- Secondary accessions
Proteomes
Phenotypes & Variants
Disruption phenotype
Leads to the loss of T-Toxin production, resulting in low virulence for maize (PubMed:16529376).
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000437635 | 1-2144 | Reducing polyketide synthase PKS2 | ||
Modified residue | 2096 | O-(pantetheine 4'-phosphoryl)serine | |||
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 10-436 | Ketosynthase family 3 (KS3) | |||
Region | 538-855 | Malonyl-CoA:ACP transacylase (MAT) | |||
Region | 924-1058 | N-terminal hotdog fold | |||
Region | 924-1214 | Dehydratase (DH) domain | |||
Domain | 924-1237 | PKS/mFAS DH | |||
Region | 1076-1237 | C-terminal hotdog fold | |||
Region | 1461-1747 | Enoyl reductase (ER) domain | |||
Region | 1771-1948 | Ketoreductase (KR) domain | |||
Domain | 2059-2136 | Carrier | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,144
- Mass (Da)231,806
- Last updated2013-06-26 v1
- MD5 ChecksumB1903A30EC0009DFDF1AFDD67917FE54
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 1359 | in Ref. 1; ABB76806 | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ186598 EMBL· GenBank· DDBJ | ABB76806.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KB733526 EMBL· GenBank· DDBJ | ENH98579.1 EMBL· GenBank· DDBJ | Genomic DNA |