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N4WHA7 · PKS2_COCH4

Function

function

Reducing polyketide synthase (PKS); part of the Tox1A locus, one of the 2 loci that mediate the biosynthesis of T-toxin, a family of linear polyketides 37 to 45 carbons in length, of which the major component is 41 carbons, and which leads to high virulence to maize (PubMed:20192833, PubMed:8953776).
One of the PKSs (PKS1 or PKS2) could synthesize a precursor, used subsequently by the other PKS as starter unit, to add additional carbons (PubMed:16529376).
Variability in the length of the final carbon backbone C35-47 could be achieved by varying the number of condensation cycles, or use of different starter or extender units or might be due to decarboxylation of the penultimate product, catalyzed by DEC1 (PubMed:12236595).
Additional proteins are required for the biosynthesis of T-toxin, including oxidoreductases RED1, RED2, RED3, LAM1 and OXI1, as well as esterase TOX9 (PubMed:20192833).

Pathway

Mycotoxin biosynthesis.

Features

Showing features for active site.

121442004006008001,0001,2001,4001,6001,8002,000
TypeIDPosition(s)Description
Active site183For beta-ketoacyl synthase activity
Active site319For beta-ketoacyl synthase activity
Active site360For beta-ketoacyl synthase activity

GO annotations

AspectTerm
Molecular Function3-oxoacyl-[acyl-carrier-protein] synthase activity
Molecular Functionfatty acid synthase activity
Molecular Functionoxidoreductase activity
Molecular Functionphosphopantetheine binding
Biological Processfatty acid biosynthetic process
Biological Processsecondary metabolite biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Reducing polyketide synthase PKS2
  • EC number
  • Alternative names
    • T-toxin biosynthesis protein PKS2

Gene names

    • Name
      PKS2
    • ORF names
      COCC4DRAFT_45941

Organism names

Accessions

  • Primary accession
    N4WHA7
  • Secondary accessions
    • Q2XW08

Proteomes

Phenotypes & Variants

Disruption phenotype

Leads to the loss of T-Toxin production, resulting in low virulence for maize (PubMed:16529376).

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_00004376351-2144Reducing polyketide synthase PKS2
Modified residue2096O-(pantetheine 4'-phosphoryl)serine

Keywords

Structure

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain10-436Ketosynthase family 3 (KS3)
Region538-855Malonyl-CoA:ACP transacylase (MAT)
Region924-1058N-terminal hotdog fold
Region924-1214Dehydratase (DH) domain
Domain924-1237PKS/mFAS DH
Region1076-1237C-terminal hotdog fold
Region1461-1747Enoyl reductase (ER) domain
Region1771-1948Ketoreductase (KR) domain
Domain2059-2136Carrier

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,144
  • Mass (Da)
    231,806
  • Last updated
    2013-06-26 v1
  • MD5 Checksum
    B1903A30EC0009DFDF1AFDD67917FE54
MTSRQNTNTPMPLAIIGMSCRFPGKVASLEDFWDMLSNSKHGYRQFPRERFNWEAFYHPNQSRKDCIDVNCGYFLDGDIAEFDAQFFKMNGTDAASFDPQGRMILECVYEALENAGVPKESIVGSKVGVFSTSNTSDYTLSLKDDIYSMPALVGVLGHACMLSNIVSNTFDLKGPSVSIDTACSSAFYALQLASQSLRSGETEMCIVSGCALNISPWRWTMLSNLTMLNPDGLSKSFDPQADAGYVRGEGAASIIVKPLDAAIRDNDRVHCVLSDIGVNHNGRTNGYTLPDARMQASLMRELQVRLDIKPDEFGFVEAHAPGTRVGDPIEISALQEVFSTSARTLEDPLLIGSVKANVGHLESSSGFPSLIKAAMMLKKGLVVPNANFENESMNSHLKEKNMRVPISTQPWPKGKTYIAINNYGFGGSNSHCIVRAPPIPQGLVSQKETRNVESDYLFVLSANDEVALRRTREQLVEFLESVDASSTTMQNTAYTLGQRRSLLSWRATVVASNIDDLIIQAASPQVIPRRVTRQPTLVFAFTGQGAQYFGVGRELLQYPVFSTTLKMASACAESFGANFSLQDELYGNEATSRINDADVSQPASTAIQIALVDLLRSWGIQPSAVVGHSSGEVAAAYAAGLLSLPGAMRIAYARGQMAIRIKKVQPDFKGGMLAVAAGPADVLPLLDIVTSGKVVIACENSPKSVTVSGDEAGLVELESLLEEDGLPHRRLAVDFPYHSTFLDPFIDDYEEAICTDDTFSNLQPTAEYFSAMAGRKVEPVTVQKPSYWASSAKFRVRFTSAAKALLRSKPSPNVVVEIGPNPTLVGSLKSILSEIKKEIPHPIEVVPSLHRGQNARTAMLKLGASLVSFGQRIDMEQVNFASGHISGQPPTLVDGIKPYPWTRSHHWIKSRVRDDDLHRPFPHHDLLGSINSSWGSKELVWKNNLDVENVPWLRDYQVASSITYPLAGYVCAAIEASKQFAMTRNLFLDRAFKGFTVRDMIIDESLVMKEGIPVELVTKLRSLPGTNFEEFEVLSWDEGQRAWKRCCRALVKCEATTDGVEQVEEMKWAESRAACHSCVGSPLLYQRLSKVGPRRTGKFRNVVDLRYGAGKTTAEVVVSDTKASMPQHYESDMTVHPTTIDGLFQCGSCIPFLDESSSVVGGSSNIWVPRSIKEFTIQTRPGEALKPEMVFRTVARVDKNERHDRSYSIDGTTDNAPICQIRIRGLKLAVEATLAPQWPAPHYGCYKIAWQNATELRSQAAQWHVLQGPGDVKNLAGSVSKKIGGTVRPLCEGVPSEASFCVVVDVGEGLLASVERESFNHIKQALTTCEGVLWVTCGAFGVSYDSTHPNAGMVTGLLRTIRSEMRASVASLDLDANASSDIEAQAALVKRVADHLAAAAQNADVQAEMEFTEKQGQLMVSRVVHDTQLDNVVHAVTGVIAPRTEPFDPEVRGFFTLQRPGMPDSLYLQRTDVPDPLDESEVEVRIAAIALDADDIHGLQGRALSGTVVRCGSTVTRVQPGDRVFGLANIDGAVRTFARAPETCLARTPANIPIDAAAALPATLGAAYHALVDLGRLVAGESVLIVAVGSALGQAAIQVALAKGALVFALAHSQEERDAAIVAGASIDRVVTTLVGLPPIQILFNPVSDANANLSMLGALAPLGRIVQVGEPSHQYPALAVGHSFSIAHLDAVADALPAQMAAILDAVVGLVDSKFVHSPPVRTVGLEYLSEALSNISETDSKKLLLVPGKNEMVKATPSCPAPPTFDPAAVYLLVGGSGGLGRVIAKWMLNNGARKIGLLSRSTSMSPDVRTLVDDAAGIGAEVFLLPCDVTSQHHLQRVIDQCVIEKGQIKGVINAAMVFKGGVFTSVSFDDFTSVVQPKVCGTWNLHHALREATLDFFILISSVAGIMGTPGHSAYASANTFLDSFAMYRMQQGLPATSLALTAVVDAGYMAENASKLQKLKYVSEFEGEILLTADVLALLGAAVTGSIASSCKGFSIIGAGFGTALKLPSYAQDPRFSTLTSNHSQDRKSKPRTTTAANTDTLVYAVDQADTKEEATQLLLAAIRDKIAQLQLIPVSDIVDDQTITELGLDSLTVMELYSWVGRLFRLRFGIQEYARLDTLEKIVDSVIVKREAAKVEAP

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict1359in Ref. 1; ABB76806

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ186598
EMBL· GenBank· DDBJ
ABB76806.1
EMBL· GenBank· DDBJ
Genomic DNA
KB733526
EMBL· GenBank· DDBJ
ENH98579.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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