N2BJ84 · N2BJ84_9ACTN
- ProteinDihydroorotase
- GenepyrC
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids430 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.
Catalytic activity
- (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H+
Cofactor
Note: Binds 2 Zn2+ ions per subunit.
Pathway
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 61 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 63 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 63-65 | substrate | |||
Binding site | 95 | substrate | |||
Binding site | 153 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 153 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 180 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 233 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 280 | substrate | |||
Active site | 307 | ||||
Binding site | 307 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 311 | substrate | |||
Binding site | 325-326 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | allantoinase activity | |
Molecular Function | dihydroorotase activity | |
Molecular Function | zinc ion binding | |
Biological Process | 'de novo' UMP biosynthetic process | |
Biological Process | purine nucleobase catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDihydroorotase
- EC number
- Short namesDHOase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Coriobacteriia > Coriobacteriales > Atopobiaceae > Atopobium
Accessions
- Primary accessionN2BJ84
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 52-423 | Amidohydrolase-related | |||
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length430
- Mass (Da)46,630
- Last updated2013-06-26 v1
- Checksum3D9B889038A56864
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AGXC01000002 EMBL· GenBank· DDBJ | EMZ41827.1 EMBL· GenBank· DDBJ | Genomic DNA |