M7XFW1 · M7XFW1_RHOT1
- ProteinFolylpolyglutamate synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids531 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis.
Catalytic activity
- (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H+
(6S)-5,6,7,8-tetrahydrofolyl-(γ-L-Glu)(n) RHEA-COMP:14738 + CHEBI:29985 + CHEBI:30616 = (6S)-5,6,7,8-tetrahydrofolyl-(γ-L-Glu)(n+1) RHEA-COMP:14740 + CHEBI:456216 + CHEBI:43474 + CHEBI:15378
Cofactor
Note: A monovalent cation.
Pathway
Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 99 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 173 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 201 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 342 | ATP (UniProtKB | ChEBI) | |||
Binding site | 361 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrial inner membrane | |
Cellular Component | mitochondrial matrix | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | tetrahydrofolylpolyglutamate synthase activity | |
Biological Process | one-carbon metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFolylpolyglutamate synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Pucciniomycotina > Microbotryomycetes > Sporidiobolales > Sporidiobolaceae > Rhodotorula
Accessions
- Primary accessionM7XFW1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Structure
Sequence
- Sequence statusComplete
- Length531
- Mass (Da)56,446
- Last updated2013-05-29 v1
- Checksum0CD2AF5F09E89525
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KB722673 EMBL· GenBank· DDBJ | EMS19033.1 EMBL· GenBank· DDBJ | Genomic DNA |