M5AVM8 · M5AVM8_CYNPY

Function

function

Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends.

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 divalent metal cation per subunit. Mg2+ or Mn2+.

GO annotations

AspectTerm
Cellular ComponentDNA recombinase complex
Cellular Componentendodeoxyribonuclease complex
Cellular Componentnucleus
Molecular Functiondouble-stranded DNA endonuclease activity
Molecular Functionhistone binding
Molecular Functionprotein homodimerization activity
Molecular Functionsequence-specific DNA binding
Molecular Functionubiquitin protein ligase activity
Molecular Functionzinc ion binding
Biological Processadaptive immune response
Biological Processchromatin organization
Biological Processpre-B cell allelic exclusion
Biological ProcessT cell differentiation in thymus
Biological ProcessV(D)J recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    V(D)J recombination-activating protein 1
  • EC number

Gene names

    • Name
      Rag-1

Organism names

Accessions

  • Primary accession
    M5AVM8

Subcellular Location

Keywords

  • Cellular component

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Belongs to the RAG1 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    403
  • Mass (Da)
    46,143
  • Last updated
    2013-05-29 v1
  • Checksum
    96922B6190F2C7CF
RYDVALVSTLKDMEEDILEGLKAHELDDYLSGPFTVVVKESCDGMGDVSEKHGCGPVVPEKAVRFSFTIMTIAVDHNKDNVRIFEENKPNSELCCKPLCLMLADESDHETLTAILSPLIAERESMKGSELMLELGGILRTFKFVFRGTGYDEKLVREVEGLEASGSVYICTLCDSTRLEASQNIVLHSITRSHKENLERYEIWRSNRHHESVDELRDRVKGVSAKPFIETLPSIDALHCDIGNAAEFYKIFQLEIGEVFKNPNASKEERKRWQSTLDKHLRKKMNLKPIMRMNGNFARKLMSQETVEAVCELVHSEERRAALRELMDLYLKMKPVWRSSCPSKECPELLCQYSYNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEG

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue403

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB754784
EMBL· GenBank· DDBJ
BAN05100.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754785
EMBL· GenBank· DDBJ
BAN05101.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754786
EMBL· GenBank· DDBJ
BAN05102.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754787
EMBL· GenBank· DDBJ
BAN05103.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754788
EMBL· GenBank· DDBJ
BAN05104.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754789
EMBL· GenBank· DDBJ
BAN05105.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754792
EMBL· GenBank· DDBJ
BAN05108.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754794
EMBL· GenBank· DDBJ
BAN05110.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754795
EMBL· GenBank· DDBJ
BAN05111.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754797
EMBL· GenBank· DDBJ
BAN05113.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754798
EMBL· GenBank· DDBJ
BAN05114.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754799
EMBL· GenBank· DDBJ
BAN05115.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754800
EMBL· GenBank· DDBJ
BAN05116.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754801
EMBL· GenBank· DDBJ
BAN05117.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754802
EMBL· GenBank· DDBJ
BAN05118.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754803
EMBL· GenBank· DDBJ
BAN05119.1
EMBL· GenBank· DDBJ
Genomic DNA
AB754805
EMBL· GenBank· DDBJ
BAN05121.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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