M4ZVJ7 · M4ZVJ7_9BRAD
- ProteinCarbamoyltransferase HypF
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids805 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Involved in the maturation of [NiFe] hydrogenases. Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases. HypF functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide.
Catalytic activity
- ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + diphosphate + H+ + phosphate
Pathway
Protein modification; [NiFe] hydrogenase maturation.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 34 | |||||
Sequence: R | ||||||
Active site | 52 | |||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | acylphosphatase activity | |
Molecular Function | carboxyl- or carbamoyltransferase activity | |
Molecular Function | double-stranded RNA binding | |
Molecular Function | ligase activity | |
Molecular Function | zinc ion binding | |
Biological Process | protein maturation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarbamoyltransferase HypF
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Bradyrhizobium
Accessions
- Primary accessionM4ZVJ7
Proteomes
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 19-106 | Acylphosphatase-like | ||||
Sequence: TVEIRVRGTVQGVGFRPTVWRLARDNELVGHVLNDSCGVLIRATGEQARIARFLSCLEANPPPLSRIESLDIHRLDEILTFEDFCIAR | ||||||
Domain | 218-403 | YrdC-like | ||||
Sequence: VPALDAAVRWIRDGKILAIRGLGGFHLACDATNAGAVDRLRARKRRFGKPFALMARDTAVIRRFAAISSRELELLQSPGAPIVLLRADGPRHLPENVAPGLATLGFMLAYTPLHKLILENFDGPLVMTSGNISDEPQVTDNRDARAKLAGIADGALLHDRDIANRIDDSVVRVMSGRARLLRRARG |
Sequence similarities
Belongs to the carbamoyltransferase HypF family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length805
- Mass (Da)87,207
- Last updated2013-05-29 v1
- ChecksumCDA5EA0F06FF4567
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP012603 EMBL· GenBank· DDBJ | BAM90365.1 EMBL· GenBank· DDBJ | Genomic DNA |