M4Z9A8 · M4Z9A8_9BRAD

Function

function

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

1914100200300400500600700800900
TypeIDPosition(s)Description
Active site132O-(5'-phospho-DNA)-tyrosine intermediate

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA negative supercoiling activity
Biological ProcessDNA topological change
Biological ProcessDNA-templated DNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA gyrase subunit A
  • EC number

Gene names

    • Name
      gyrA
    • ORF names
      S58_40210

Organism names

Accessions

  • Primary accession
    M4Z9A8

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain, motif.

TypeIDPosition(s)Description
Region1-20Disordered
Domain21-500DNA topoisomerase type IIA
Motif561-567GyrA-box

Sequence similarities

Belongs to the type II topoisomerase GyrA/ParC subunit family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    914
  • Mass (Da)
    100,849
  • Last updated
    2013-05-29 v1
  • Checksum
    CD397E0C66CE8858
MSDKEDEKPGEPGGPSDIRPVSILDEMKRSYLDYAMSVIVSRALPDARDGLKPVHRRILYSMYEQGHTPDKKYVKSARVVGDVIGKYHPHGDQSIYDAMVRMAQDFSMRVPLIDGQGNFGSVDGDPPAAYRYTEAKLAKSAIPLLDDIDKDTVDFQANYDNSDKEPSVLPAKFPNLLVNGAGGIAVGMATNIPPHNLGEVIDACVALIDNPALTIDDLINIIPGPDFPTGGIILGRAGIRSAYHLGRGSIMMRGKVTIDTIRKDREAIIVSEIPYQVNKATMVERIAELVREKKIEGISDLRDESDRDGYRVVIELKRDAVPDVVLNQLYKFTPLQTSFAANMLALDGGRPQTMNLKDLLTIFVAFRERVVTRRTKYLLNKARDRAHILVGLAVAVANIDEVIRVIRTSPDPATAREALMSRDWPARDVEAIITLIDDPRHRLSEDGTIRLSFEQAKAILDLRLQRLTALGREEISDELDKLKVEINDYLDILRSRARVQGIIKDELGAVKAEFGTPRRTLIAEQEGEVEDEDLIQREDMVVTVSHAGYTKRVPLSAYRAQRRGGKGRSGMQTREEDFVSRLFVASTHTPVLFFSSRGQVYKEKVWRLPIAAPNARGKALINILPLEQGERITTIMPLPEDEASWANLDVMFATTGGNVRRNKLSDFVDVRRSGIIAMKLDEGEEIVNVQICTERDDVLLTASGGQCIRFPVTDVRVFTGRTSMGVRGIALAGGDKVISLSILRHVETTSDERTAYLKMRRAMAGEVASEEPVESEGEEAAAGSIQLSQERYVEMSAQEQVVLTVSVNGYGKRTSSYEYRTTGRGGKGIVAMSVNNRNGKLVASFPVEDGDQIMLVTDKGQLIRCPVDGIRIAGRSTQGVIVFNTADDEHVVSVEHIGEETENGNGASEQANGE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012603
EMBL· GenBank· DDBJ
BAM90007.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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