M4Z4A5 · M4Z4A5_9BRAD

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

1715100200300400500600700
TypeIDPosition(s)Description
Binding site49-53NAD+ (UniProtKB | ChEBI)
Binding site98-99NAD+ (UniProtKB | ChEBI)
Binding site131NAD+ (UniProtKB | ChEBI)
Active site133N6-AMP-lysine intermediate
Binding site154NAD+ (UniProtKB | ChEBI)
Binding site191NAD+ (UniProtKB | ChEBI)
Binding site307NAD+ (UniProtKB | ChEBI)
Binding site331NAD+ (UniProtKB | ChEBI)
Binding site436Zn2+ (UniProtKB | ChEBI)
Binding site438Zn2+ (UniProtKB | ChEBI)
Binding site466Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      S58_19670

Organism names

Accessions

  • Primary accession
    M4Z4A5

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain637-710BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    715
  • Mass (Da)
    79,814
  • Last updated
    2013-05-29 v1
  • Checksum
    D581AE92F59E9F4D
MPKTTKPKKPVDVADLTKAQAKVEWKRLAIELETHDRLYYQDDQPKISDAAYDELRRRFNAIEKRFPELVSSESPSQKVGAAPSGRFKKVRHAVPMLSLDNAFAEEDVRDFAGRIARFLKLADDRIDFSAEPKIDGLSMSLRYERGELVTAATRGDGAEGEDVTANIRTLKDVPHKLHGRDLPDICEVRGEVYMTKQAFLALNERQKEAGDTVFANPRNSAAGSLRQKDPTITASRPLGFFAYAWGEMSEMPAETQSGMIKWFERCGFTTNPLTRLCHSVEELIAFHHQIEEQRAELDYDIDGVVYKVDRIDWQERLGFVSRTPRWGIAHKFPAERAMTVLKDIEIQVGRTGSFTPVGKLEPVGVGGVIVQNVTLHNEDYIKGIGNKGEVLREGRDIRIGDTVVIQRAGDVIPQVVDVVIDKRPADAGEFKLPKTCPCPLHTDVVREEIATGEEGSRARCTGEFACPFQKIQHLLLFVSRRAFDIDGLGEKQIEFFFEKEWVREPADIFTLAARNARLKLEEIEGYGETSVRNLFNAIDARREIALERFIYALGMRHVGETTALALARGYGSWDAFHDACLKVAKGDEEAIAEMDALDQIGDTVIKSIAAYFGEDHNLGIVERLTKEVKILDAEKPKRNSPIATKTVVFTGTLEKMTRDEAKATAERLGAKVSGSVSKKTDYVVAGPGAGSKLKDAQKHGVQVLTEDEWLQLIGE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012603
EMBL· GenBank· DDBJ
BAM87974.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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