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M4HYP3 · MISO1_PINCO

Function

function

Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in diterpene (C20) olefins biosynthesis. Monofunctional enzyme lacking the DXDD motif in the class II active site relevant for the cyclization of geranylgeranyl diphosphate (GGPP). Requires +-copalyl diphosphate (+-CPP) as substrate, but no activity with GGPP or ent-CPP. Isopimaradiene is the major products of the enzyme followed by sandaracopimaradiene.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 3 Mg2+ ions per subunit.

Pathway

Terpene metabolism; oleoresin biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site628Mg2+ 1 (UniProtKB | ChEBI)
Binding site628Mg2+ 2 (UniProtKB | ChEBI)
Binding site632Mg2+ 1 (UniProtKB | ChEBI)
Binding site632Mg2+ 2 (UniProtKB | ChEBI)
Binding site772Mg2+ 3 (UniProtKB | ChEBI)
Binding site776Mg2+ 3 (UniProtKB | ChEBI)
Binding site780Mg2+ 3 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Molecular Functionmagnesium ion binding
Molecular Functionterpene synthase activity
Biological Processdefense response
Biological Processditerpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Monofunctional isopimaradiene synthase, chloroplastic
  • EC number
  • Short names
    PcmIso1

Gene names

    • Name
      TPS-mISO1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Pinopsida > Pinidae > Conifers I > Pinales > Pinaceae > Pinus > Pinus subgen. Pinus

Accessions

  • Primary accession
    M4HYP3

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

Type
IDPosition(s)Description
Transit peptide1-64Chloroplast
ChainPRO_000043141365-876Monofunctional isopimaradiene synthase, chloroplastic

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif628-632DDXXD motif

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity in the class I active site, presumably through binding to Mg2+.

Sequence similarities

Belongs to the terpene synthase family. Tpsd subfamily.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    876
  • Mass (Da)
    100,687
  • Last updated
    2013-05-29 v1
  • MD5 Checksum
    18CACA1BD5C6F5342C64BF564FD4E73D
MAMPSYSSLSSHISITTTHTRPHPIFPCYNDTQSIPRFFISSDTGSSASKQRNIYLRLGSRKIIAGVGEGATSLSSHSDKMKTDSFPDPKLAKRDFPPGFWKDDIIDSIMSSNKVAAADEERVETLISEIKSMFRGMGDGETTPSAYDTAWVAKIPALDGSDHPHFPQTLQWILRNQLKDGSWGEEHHFLTYDRLLATLACIITLTVWRTGKTQVQKGIEFFKKHAAMMEDEADHRQPSGFEFVFPAMINEAKSLCLDLPYELPFIKQIIKKREAKLKRIPTDLLYTVPTIFLYYLEGLQEIVEWHKIIKLQSKDGSFLSSPASTAAVFMSTGNTKCLEFLNFVLMKFGNHAPCHYPIDLLERLWAVDTVQRLGIDRYFKEEIKEALDYIYSHWGERGIGWARENPVADIGVTAMGLRILRLNGYNVSSDVLRTFRDENGEFFSFMGQTERGVIDMLNLNRCSHVAFPGETVMEEAKHCTERYLWNALEDVDALDKWGLKKNIRGEVEYALKYPWLRSLPRLEARSYIENYGPNDAWLGKTMYIMPYINNGKYLELAKLDFNNVQSIHQKELRELRRWWKSSGFAELDFTRDRVAEIFFSIASSMFEPELATCRDVYTKSTICTVVLDDLYDAHGSVEDIKLFNEAVKRWDLFLLDRMPEHIKICFLGLYNLVNEIAEEGRKRQGRDVLGYIRNLWEIQLETFMKEAEWSEAKYVPSFHEYIETASVSIAGATLVLFGVLFTGEVLTDHILSQIDYRSKFAYLMGLTGRLINDTKTYQAERGEGEVASAIQCYMKDHPEFSEEEALKQIYTLMENALSDLKEEFLKAKDVPDKCKRLVFDYARSMQLFYQQGDGFTLAPNMEIKQHVKKILFEPVP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JQ240314
EMBL· GenBank· DDBJ
AFU73866.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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