M4HYP3 · MISO1_PINCO
- ProteinMonofunctional isopimaradiene synthase, chloroplastic
- GeneTPS-mISO1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids876 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in diterpene (C20) olefins biosynthesis. Monofunctional enzyme lacking the DXDD motif in the class II active site relevant for the cyclization of geranylgeranyl diphosphate (GGPP). Requires +-copalyl diphosphate (+-CPP) as substrate, but no activity with GGPP or ent-CPP. Isopimaradiene is the major products of the enzyme followed by sandaracopimaradiene.
Catalytic activity
- +-copalyl diphosphate = isopimara-7,15-diene + diphosphate
Cofactor
Note: Binds 3 Mg2+ ions per subunit.
Pathway
Terpene metabolism; oleoresin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 628 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 628 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 632 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 632 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 772 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 776 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 780 | Mg2+ 3 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | magnesium ion binding | |
Molecular Function | terpene synthase activity | |
Biological Process | defense response | |
Biological Process | diterpenoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMonofunctional isopimaradiene synthase, chloroplastic
- EC number
- Short namesPcmIso1
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Pinopsida > Pinidae > Conifers I > Pinales > Pinaceae > Pinus > Pinus subgen. Pinus
Accessions
- Primary accessionM4HYP3
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transit peptide | 1-64 | Chloroplast | |||
Chain | PRO_0000431413 | 65-876 | Monofunctional isopimaradiene synthase, chloroplastic | ||
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 628-632 | DDXXD motif | |||
Domain
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity in the class I active site, presumably through binding to Mg2+.
Sequence similarities
Belongs to the terpene synthase family. Tpsd subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length876
- Mass (Da)100,687
- Last updated2013-05-29 v1
- MD5 Checksum18CACA1BD5C6F5342C64BF564FD4E73D
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JQ240314 EMBL· GenBank· DDBJ | AFU73866.1 EMBL· GenBank· DDBJ | mRNA |