M2YKT1 · MET1_DOTSN
- ProteinHps1-dma1 cluster O-methyltransferase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids433 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
O-methyltransferase; part of the hps1-dma1 gene cluster that probably mediates the biosynthesis a derivative of cyclopiazonic acid (CPA) (Probable). The hybrid polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) nps1 might incorporates acetyl-CoA, malonyl-CoA, and tryptophan (Trp) and utilizes a C-terminal redox-incompetent reductase domain to make and release the tryptophan tetramic acid, cyclo-acetoacetyl-L-tryptophan (c-AATrp), as the first intermediate in the pathway (By similarity).
In addition, the cluster also includes the tryptophan dimethylallyltransferase dma1, the FAD-dependent oxidoreductase toxD, the cytochrome P450 monooxygenase cyp3.1 and the methyltransferase DOTSEDRAFT_139328; the latter 2 being not present in all CPA-producing fungi but involved in additional modifications that occur in biosynthesis the of a range of CPA and CPA-like products (Probable). Further studies are required to clarify whether the CPA-like hps1-dma1 cluster is functional or a non-functional relic reflecting evolution of D.septosporum (Probable)
In addition, the cluster also includes the tryptophan dimethylallyltransferase dma1, the FAD-dependent oxidoreductase toxD, the cytochrome P450 monooxygenase cyp3.1 and the methyltransferase DOTSEDRAFT_139328; the latter 2 being not present in all CPA-producing fungi but involved in additional modifications that occur in biosynthesis the of a range of CPA and CPA-like products (Probable). Further studies are required to clarify whether the CPA-like hps1-dma1 cluster is functional or a non-functional relic reflecting evolution of D.septosporum (Probable)
Pathway
Secondary metabolite biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | O-methyltransferase activity | |
Biological Process | methylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHps1-dma1 cluster O-methyltransferase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Dothideomycetidae > Mycosphaerellales > Mycosphaerellaceae > Dothistroma
Accessions
- Primary accessionM2YKT1
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000447731 | 1-433 | Hps1-dma1 cluster O-methyltransferase | |||
Sequence: MGGSDHTKTSSQSTLRSLSDEISSLTDVVAGFLESNGHPERSLNSTDSVRLSDAPEDVEAARRRLVTALHEMTLLTMSPFEAVRDILLEVSSLPALHAISHFEIIDHVPLDGEISYAELARKINVPQRRLTRMLRAAMSRSIFQEPRPGYIAHNSLSAAMVHSKWLRYHAASTMENFLPAAPKFVEQIERFGDRETRCTSPAGIAFNTETDCIQYLLSQPKHQQVLVNLMKHTGEISGMGPQHLTEHYDWPKASDQIIVDVGGASGSVSRAIACGVPSVRFVVQDRADAVRQGESETPSELKDRFTFQEYDFFQTQPVKNADVYFLRWILHDWPDEDAVTILRQVALAMGPTSKMLIAERLVLLPGEGDPWDQKIATSMDMFMMAFNGSERTLEHFQSLIENTGEAIEISRVIRRPNGVQYSLIEVARKDSER |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 36-55 | Disordered | ||||
Sequence: NGHPERSLNSTDSVRLSDAP |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length433
- Mass (Da)48,521
- Last updated2013-05-01 v1
- Checksum31FDF08F2A035875
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KB446545 EMBL· GenBank· DDBJ | EME39475.1 EMBL· GenBank· DDBJ | Genomic DNA |