M2YKT1 · MET1_DOTSN

Function

function

O-methyltransferase; part of the hps1-dma1 gene cluster that probably mediates the biosynthesis a derivative of cyclopiazonic acid (CPA) (Probable). The hybrid polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) nps1 might incorporates acetyl-CoA, malonyl-CoA, and tryptophan (Trp) and utilizes a C-terminal redox-incompetent reductase domain to make and release the tryptophan tetramic acid, cyclo-acetoacetyl-L-tryptophan (c-AATrp), as the first intermediate in the pathway (By similarity).
In addition, the cluster also includes the tryptophan dimethylallyltransferase dma1, the FAD-dependent oxidoreductase toxD, the cytochrome P450 monooxygenase cyp3.1 and the methyltransferase DOTSEDRAFT_139328; the latter 2 being not present in all CPA-producing fungi but involved in additional modifications that occur in biosynthesis the of a range of CPA and CPA-like products (Probable). Further studies are required to clarify whether the CPA-like hps1-dma1 cluster is functional or a non-functional relic reflecting evolution of D.septosporum (Probable)

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site, active site.

143350100150200250300350400
TypeIDPosition(s)Description
Binding site285S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site331Proton acceptor

GO annotations

AspectTerm
Molecular FunctionO-methyltransferase activity
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Hps1-dma1 cluster O-methyltransferase
  • EC number

Gene names

    • ORF names
      DOTSEDRAFT_139328

Organism names

Accessions

  • Primary accession
    M2YKT1

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004477311-433Hps1-dma1 cluster O-methyltransferase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region36-55Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    433
  • Mass (Da)
    48,521
  • Last updated
    2013-05-01 v1
  • Checksum
    31FDF08F2A035875
MGGSDHTKTSSQSTLRSLSDEISSLTDVVAGFLESNGHPERSLNSTDSVRLSDAPEDVEAARRRLVTALHEMTLLTMSPFEAVRDILLEVSSLPALHAISHFEIIDHVPLDGEISYAELARKINVPQRRLTRMLRAAMSRSIFQEPRPGYIAHNSLSAAMVHSKWLRYHAASTMENFLPAAPKFVEQIERFGDRETRCTSPAGIAFNTETDCIQYLLSQPKHQQVLVNLMKHTGEISGMGPQHLTEHYDWPKASDQIIVDVGGASGSVSRAIACGVPSVRFVVQDRADAVRQGESETPSELKDRFTFQEYDFFQTQPVKNADVYFLRWILHDWPDEDAVTILRQVALAMGPTSKMLIAERLVLLPGEGDPWDQKIATSMDMFMMAFNGSERTLEHFQSLIENTGEAIEISRVIRRPNGVQYSLIEVARKDSER

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KB446545
EMBL· GenBank· DDBJ
EME39475.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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