M2XJ44 · CYP31_DOTSN

Function

function

Cytochrome P450 monooxygenase; part of the hps1-dma1 gene cluster that probably mediates the biosynthesis a derivative of cyclopiazonic acid (CPA) (Probable). The hybrid polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) nps1 might incorporates acetyl-CoA, malonyl-CoA, and tryptophan (Trp) and utilizes a C-terminal redox-incompetent reductase domain to make and release the tryptophan tetramic acid, cyclo-acetoacetyl-L-tryptophan (c-AATrp), as the first intermediate in the pathway (By similarity).
In addition, the cluster also includes the tryptophan dimethylallyltransferase dma1, the FAD-dependent oxidoreductase toxD, the cytochrome P450 monooxygenase cyp3.1 and the methyltransferase DOTSEDRAFT_139328; the latter 2 being not present in all CPA-producing fungi but involved in additional modifications that occur in biosynthesis the of a range of CPA and CPA-like products (Probable). Further studies are required to clarify whether the CPA-like hps1-dma1 cluster is functional or a non-functional relic reflecting evolution of D.septosporum (Probable)

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site260Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Hps1-dma1 cluster cytochrome P450 monooxygenase cyp3.1

Gene names

    • Name
      cyp3.1
    • ORF names
      DOTSEDRAFT_75219

Organism names

Accessions

  • Primary accession
    M2XJ44

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004477301-317Hps1-dma1 cluster cytochrome P450 monooxygenase cyp3.1

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region183-205Disordered

Sequence similarities

Belongs to the cytochrome P450 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    317
  • Mass (Da)
    35,346
  • Last updated
    2013-05-01 v1
  • Checksum
    B69F5F3272E94871
MGTYYRRKDQFLLAKIHEAEARMTGEDSSGSSARIRRRSALDHFVRQEMDAAARAGRAPDFLHGPIRDELLGYIIAGHDSSTSALSWALKYLTTDARVQHALRQHLHAVHAASWREGRTPSVAEITSIRAPYLDAVIQEVFRCAYTTPFTLREATRDTQIMGHFVPKGTTVWFSTSGPSFTRPAIETDRKTSSHSRSPTALADKQRVPAWSPQDVALFRPERWLRPDPGPSDPPDFAYSHVDFNGNAGPMMAFGSGPRGCCGKRLVYLSMRILVTLVLWDFDLLPCPTRLSSDESNHGTASHPKQCYLRLATTTHGP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KB446545
EMBL· GenBank· DDBJ
EME39477.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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