M1W426 · TCPG_CLAP2

Function

function

Glutathione S-transferase; part of the gene cluster that mediates the biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) lacking the reactive thiol group important for toxicity (PubMed:27390873).
Firstly, L-tyrosine is prenylated by tcpD, before undergoing condensation with L-glycine in a reaction catalyzed by the NRPS tcpP leading to the diketopiperazine (DKP) backbone (PubMed:27390873).
Afterwards the alpha-carbon of tyrosine is oxidized by the cytochrome P450 tcpC to form a hydroxyl group (PubMed:27390873).
However, in contrast other ETP biosynthesis pathways studied so far, tcpC is not able to bishydroxylate the DKP at both alpha-carbon positions, but hydroxylates the alpha-carbon of the tyrosine part and the nitrogen of the glycine part (PubMed:27390873).
The next steps involve an alpha,beta-elimination reaction catalyzed by tcpI, a methylation by the methyltransferase tcpN the action of the four enzyme cascade tcpG/K/J/I (PubMed:27390873).
Due to a dysfunctional cytochrome P450 monooxygenase tcpC, the pathway leads to the biosynthesis of probable non-toxic metabolites lacking the reactive thiol group (PubMed:27390873).

Catalytic activity

Pathway

Secondary metabolite biosynthesis.

GO annotations

AspectTerm
Molecular Functionglutathione transferase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutathione S-transferase tcpG
  • EC number
  • Alternative names
    • Thioclapurine biosynthesis protein G

Gene names

    • Name
      tcpG
    • ORF names
      CPUR_02676

Organism names

Accessions

  • Primary accession
    M1W426

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004377261-249Glutathione S-transferase tcpG

Expression

Induction

Expression is increases towards the late stages of rye plants infection (PubMed:27390873).
Expression is positively regulated by the thioclapurine cluster-specific transcription factor tcpZ (PubMed:27390873).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain20-109GST N-terminal
Domain115-249GST C-terminal

Sequence similarities

Belongs to the GST superfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    249
  • Mass (Da)
    28,456
  • Last updated
    2013-05-01 v1
  • Checksum
    602611604899E39D
MEAQQDFTKHPEVAQDRLVLYVRKAIPAPTANSLKPLMILEALEIPYSIHLISSLSQETWYHEINPYKQLPALEDIDLVETSGGSKRRLNVFDSSAMLIYLCDKHDKDGLFIGRNATERAQVTSWLIAYAAGLGATGEWWLKMRHDENLKPALRVIENAIRREYDILEKRLGEPGQRWIALADRPTVADFAIQPLANPRVARNAAIDFEAWPRTKAWSEAVDRLAYIDRAKRLNNKLGMTEEEIELHGR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CAGA01000011
EMBL· GenBank· DDBJ
CCE28985.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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