M1VV65 · TCPJ_CLAP2

Function

function

Dipeptidase; part of the gene cluster that mediates the biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) lacking the reactive thiol group important for toxicity (PubMed:27390873).
Firstly, L-tyrosine is prenylated by tcpD, before undergoing condensation with L-glycine in a reaction catalyzed by the NRPS tcpP leading to the diketopiperazine (DKP) backbone (PubMed:27390873).
Afterwards the alpha-carbon of tyrosine is oxidized by the cytochrome P450 tcpC to form a hydroxyl group (PubMed:27390873).
However, in contrast other ETP biosynthesis pathways studied so far, tcpC is not able to bishydroxylate the DKP at both alpha-carbon positions, but hydroxylates the alpha-carbon of the tyrosine part and the nitrogen of the glycine part (PubMed:27390873).
The next steps involve an alpha,beta-elimination reaction catalyzed by tcpI, a methylation by the methyltransferase tcpN the action of the four enzyme cascade tcpG/K/J/I (PubMed:27390873).
Due to a dysfunctional cytochrome P450 monooxygenase tcpC, the pathway leads to the biosynthesis of probable non-toxic metabolites lacking the reactive thiol group (PubMed:27390873).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site46Zn2+ (UniProtKB | ChEBI); catalytic
Binding site48Zn2+ (UniProtKB | ChEBI); catalytic
Binding site158Zn2+ (UniProtKB | ChEBI); catalytic
Binding site185substrate
Binding site259substrate
Binding site318substrate

GO annotations

AspectTerm
Molecular Functionmetal ion binding
Molecular Functionmetallodipeptidase activity
Biological Processproteolysis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dipeptidase tcpJ
  • EC number
  • Alternative names
    • Thiocalpurine biosynthesis protein J

Gene names

    • Name
      tcpJ
    • ORF names
      CPUR_02673

Organism names

Accessions

  • Primary accession
    M1VV65

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004377041-425Dipeptidase tcpJ

Expression

Induction

Expression is positively regulated by the thioclapurine cluster-specific transcription factor tcpZ (PubMed:27390873).

Structure

Family & Domains

Sequence similarities

Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    425
  • Mass (Da)
    47,461
  • Last updated
    2013-05-01 v1
  • Checksum
    0A786B472BC291E5
MATAAQPSLELALELMSKVPLIGNISQSTHKIPIQCTGLTREQDGHNDWMHMIRAYYDFQVDDRFQPTKDLAGHVDLKRLVQGRAGAVFWSVYVECPKGENDFSDAVHHASMRDTFQQIDLLQRIMELYSDRMEMAHKADDVMRIFRSGKCASLMGAEGLHQLGNSSSVLRIFHRLGVRYVTLAHAKNNLYVDSATSEAPIHHGLSPQGRDMVREMNRIGMIVDLSHVSEKAMVDALDVSLAPVIFSHSSAYALVPHVRNVPDHVLDRLKQNRGIIMISFIPWLTNKDPEKATVENVVDHVLHVGNRIGFDHLGLGSDFDGMPSHVQGLEDVSKYPNVVAAMLQRGISTENVEKIMGMNVIRVLREVEDVAASQKGLLPVLEDAVPQLWDDGIRAYVKKLYPHAEHDRTGASETTTVDKAIEKDV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CAGA01000011
EMBL· GenBank· DDBJ
CCE28982.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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