M1J8U6 · IFI4E_PRUDO
- ProteinEukaryotic translation initiation factor isoform 4E
- GeneeIF(iso)4E
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids213 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (By similarity).
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (By similarity).
Key component of recessive resistance to potyviruses such as the plum pox virus (PPV) strain D (PubMed:23382802).
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (By similarity).
Key component of recessive resistance to potyviruses such as the plum pox virus (PPV) strain D (PubMed:23382802).
(Microbial infection) Susceptibility host factor required for viral infection by recruiting viral RNAs to the host ribosomal complex via an interaction with viral genome-linked protein (VPg).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 56-61 | N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI) | ||||
Sequence: QGAAWG | ||||||
Binding site | 88 | N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 106-107 | N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI) | ||||
Sequence: WE | ||||||
Binding site | 157-162 | N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI) | ||||
Sequence: RQRQDK | ||||||
Binding site | 202-205 | N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI) | ||||
Sequence: KRER |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | RNA binding | |
Molecular Function | translation initiation factor activity | |
Biological Process | defense response to virus |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameEukaryotic translation initiation factor isoform 4E
- Short nameseIF(iso)-4E ; eIF(iso)4E
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Rosales > Rosaceae > Amygdaloideae > Amygdaleae > Prunus
Accessions
- Primary accessionM1J8U6
Phenotypes & Variants
Disruption phenotype
(Microbial infection) Increased resistance to plum pox virus (PPV) strain D.
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454074 | 1-213 | Eukaryotic translation initiation factor isoform 4E | |||
Sequence: MATEVAAAVPPPQLDAEENSGLEAAAAEAKIQPSSGPHKLERKWTFWFDNQSKPKQGAAWGSSLRKAYTFETVQEFWCLYDQVFKPSKFPPNADFHLFRAGVEPKWEDPECANGGKWTVTSRSKASLDTMWLETLMALIGEQFDEADEICGVVASVRQRQDKLALWTRNAANEAAQMGIGRKWKEIIDVTDKITYSFHDDSKRERSAKPRYNV | ||||||
Disulfide bond | 111↔150 | |||||
Sequence: CANGGKWTVTSRSKASLDTMWLETLMALIGEQFDEADEIC |
Post-translational modification
According to the redox status, the Cys-111-Cys-150 disulfide bridge may have a role in regulating protein function by affecting its ability to bind capped mRNA.
Keywords
- PTM
Expression
Tissue specificity
Mostly expressed in leaves, flower buds, leaf buds and anthers, to a lower extent in roots, stems and green immature fruit, and, at low levels, in petals.
Interaction
Subunit
EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions (By similarity).
It is composed of at least EIF4A, EIF4E and EIF4G (By similarity).
EIF4E is also known to interact with other partners. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F (By similarity).
Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28 (By similarity).
It is composed of at least EIF4A, EIF4E and EIF4G (By similarity).
EIF4E is also known to interact with other partners. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F (By similarity).
Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28 (By similarity).
(Microbial infection) Interacts with potyvirus viral genome-linked protein (VPg) of plum pox virus (PPV) strain D both in nucleus and cytoplasm; this interaction is possible in susceptible hosts but is impaired in resistant plants.
Structure
Sequence
- Sequence statusComplete
- Length213
- Mass (Da)24,074
- Last updated2013-05-01 v1
- ChecksumBD30434D86BE4EF4