M1J8U6 · IFI4E_PRUDO

  • Protein
    Eukaryotic translation initiation factor isoform 4E
  • Gene
    eIF(iso)4E
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (By similarity).
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (By similarity).
Key component of recessive resistance to potyviruses such as the plum pox virus (PPV) strain D (PubMed:23382802).
(Microbial infection) Susceptibility host factor required for viral infection by recruiting viral RNAs to the host ribosomal complex via an interaction with viral genome-linked protein (VPg).

Features

Showing features for binding site.

121320406080100120140160180200
TypeIDPosition(s)Description
Binding site56-61N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI)
Binding site88N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI)
Binding site106-107N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI)
Binding site157-162N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI)
Binding site202-205N7-methylguanosine 5'-triphosphate group (UniProtKB | ChEBI); m7GTP residue in mRNA cap of mRNA (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular FunctionRNA binding
Molecular Functiontranslation initiation factor activity
Biological Processdefense response to virus

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Eukaryotic translation initiation factor isoform 4E
  • Short names
    eIF(iso)-4E
    ; eIF(iso)4E
  • Alternative names
    • eIF-(iso)4F 25 kDa subunit
    • eIF-(iso)4F p28 subunit
    • mRNA cap-binding protein

Gene names

    • Name
      eIF(iso)4E

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Rosales > Rosaceae > Amygdaloideae > Amygdaleae > Prunus

Accessions

  • Primary accession
    M1J8U6

Subcellular Location

Cytoplasm
Nucleus
Note: (Microbial infection) Binds to potyvirus viral genome-linked protein (VPg) in cytoplasm and nucleus.

Keywords

Phenotypes & Variants

Disruption phenotype

(Microbial infection) Increased resistance to plum pox virus (PPV) strain D.

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00004540741-213Eukaryotic translation initiation factor isoform 4E
Disulfide bond111↔150

Post-translational modification

According to the redox status, the Cys-111-Cys-150 disulfide bridge may have a role in regulating protein function by affecting its ability to bind capped mRNA.

Keywords

Expression

Tissue specificity

Mostly expressed in leaves, flower buds, leaf buds and anthers, to a lower extent in roots, stems and green immature fruit, and, at low levels, in petals.

Interaction

Subunit

EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions (By similarity).
It is composed of at least EIF4A, EIF4E and EIF4G (By similarity).
EIF4E is also known to interact with other partners. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F (By similarity).
Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28 (By similarity).
(Microbial infection) Interacts with potyvirus viral genome-linked protein (VPg) of plum pox virus (PPV) strain D both in nucleus and cytoplasm; this interaction is possible in susceptible hosts but is impaired in resistant plants.

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-37Disordered

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    213
  • Mass (Da)
    24,074
  • Last updated
    2013-05-01 v1
  • Checksum
    BD30434D86BE4EF4
MATEVAAAVPPPQLDAEENSGLEAAAAEAKIQPSSGPHKLERKWTFWFDNQSKPKQGAAWGSSLRKAYTFETVQEFWCLYDQVFKPSKFPPNADFHLFRAGVEPKWEDPECANGGKWTVTSRSKASLDTMWLETLMALIGEQFDEADEICGVVASVRQRQDKLALWTRNAANEAAQMGIGRKWKEIIDVTDKITYSFHDDSKRERSAKPRYNV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JX137117
EMBL· GenBank· DDBJ
AGE81988.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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