M0FPE1 · M0FPE1_9EURY

Function

function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site68[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site72[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site75[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site208[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site280[2Fe-2S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionbiotin synthase activity
Molecular Functioniron ion binding
Biological Processbiotin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Biotin synthase
  • EC number

Gene names

    • Name
      bioB
    • ORF names
      C459_16001

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC BAA-645
  • Taxonomic lineage
    Archaea > Euryarchaeota > Stenosarchaea group > Halobacteria > Halobacteriales > Haloferacaceae > Haloferax

Accessions

  • Primary accession
    M0FPE1

Proteomes

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain50-276Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    352
  • Mass (Da)
    37,836
  • Last updated
    2013-04-03 v1
  • Checksum
    AE73DDD414282AE9
MVYETGNPTVDDAVRRVLDGDELDRTDGIALIAQPVEALAAGADYVRSVRSDGTVDACSIVNAKAGDCAEDCGFCAQSAHFDTGIETHGFLGPEAVLDAARRAERDGAQRFGIVVAEKGVSKERRPDEWAEVLAAIRLVRDETSVEIDASLGLLTEEEARILSAEGVNHYNHNIETSPRYFDEVVGTHDFEDRLVTLRRAKDAGMDLCAGVILGMGETPADRVDAALELQKVGVESLPVNVLNPVPGTPLGDADHADITTTELVKTVAVYRLLHPDARVRLTGGREVNLAPDEQHLPFEAGADGVLTGDYLTTDGQSPAADIEVVERAGLEPNRAVNDFDVDAVKRRESRDD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AOLE01000035
EMBL· GenBank· DDBJ
ELZ61143.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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