L9WEM2 · L9WEM2_9EURY

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site45L-glutamine (UniProtKB | ChEBI)
Binding site216L-glutamine (UniProtKB | ChEBI)
Binding site218L-glutamine (UniProtKB | ChEBI)
Active site243Nucleophile
Binding site244L-glutamine (UniProtKB | ChEBI)
Binding site247L-glutamine (UniProtKB | ChEBI)
Binding site285L-glutamine (UniProtKB | ChEBI)
Binding site287L-glutamine (UniProtKB | ChEBI)
Binding site288L-glutamine (UniProtKB | ChEBI)
Active site325
Active site327

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      C494_11935

Organism names

  • Taxonomic identifier
  • Strain
    • JCM 10635
  • Taxonomic lineage
    Archaea > Euryarchaeota > Stenosarchaea group > Halobacteria > Halobacteriales > Natrialbaceae > Natronorubrum

Accessions

  • Primary accession
    L9WEM2

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-167CPSase
Domain2-122Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.
Belongs to the CarB family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    358
  • Mass (Da)
    38,688
  • Last updated
    2013-04-03 v1
  • Checksum
    229D08C1D54ED8F3
MTAAYVALEGGHVLEGRGRASGTARGELVFTTAYTGYEESLTDPSYEEQILTFSYPLIGNYGVREERFESDRIHPRAVLAKELTDDVTEWLESEDVPAVDHLDTREVVTDIRDGGAMKCGIAVGDDVTEEDALEQLEQCKAMSDHTDIGAQVSVDETVVHGEDNDGDTVALVDCGAKGSIIDSLVERNATVHVLPHDATEADIEAVDPDILFISNGPGDPANYEEAIDIVDAFVEETPVAGICLGQQIVAEALGGTTEKMTFGHRGVNQPVMDLESGRVVMTTQNHGYTVAEPGDYLEVTQINVNDDTPEGLDGVEHDIITRQYHPEANPGPQDTLDFFDDVLAMADPQNQRAVPADD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AOHY01000034
EMBL· GenBank· DDBJ
ELY47909.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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