L9LBF6 · L9LBF6_TUPCH
- ProteinrRNA biogenesis protein RRP36
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids348 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA.
Component of the 90S pre-ribosome involved in the maturation of rRNAs. Required for early cleavages of the pre-RNAs in the 40S ribosomal subunit maturation pathway.
Catalytic activity
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H+This reaction proceeds in the forward direction.
- a 1-(9Z-octadecenoyl)-2-acyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H+This reaction proceeds in the forward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | nuclear membrane | |
Cellular Component | nucleolus | |
Cellular Component | plasma membrane | |
Cellular Component | ribonucleoprotein complex | |
Molecular Function | carboxylic ester hydrolase activity | |
Molecular Function | palmitoyl-(protein) hydrolase activity | |
Biological Process | protein depalmitoylation | |
Biological Process | rRNA processing |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended namerRNA biogenesis protein RRP36
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Scandentia > Tupaiidae > Tupaia
Accessions
- Primary accessionL9LBF6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Associates with 90S and pre-40S pre-ribosomal particles.
Homodimer.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 159-251 | Phospholipase/carboxylesterase/thioesterase | |||
Coiled coil | 258-294 | ||||
Sequence similarities
Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.
Belongs to the RRP36 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length348
- Mass (Da)38,883
- Last updated2013-04-03 v1
- Checksum7960C8F3B86F0200
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KB320451 EMBL· GenBank· DDBJ | ELW71989.1 EMBL· GenBank· DDBJ | Genomic DNA |